Dxo (NM_033613) Mouse Untagged Clone

CAT#: MC207839

Dom3z (untagged) - Mouse DOM-3 homolog Z (C. elegans) (Dom3z), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Dxo
• Synonyms: Dom3z; NG6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC207839 representing NM_033613
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAACCCAGAGGGACTAAGAGAAAAGCGGAGAAGACAGAGGTGGAAAAGCCTTTGCACAAACTCCCCC
  GGGCAGTCCCTTTACTGCGGACACAGCCCTCTCTGTACTCGGGACCCTTCCCGTTCTACCGGCGTCCATC
  CGAACTGGGCTGCTTCTCCCTGGACGCACAACGCCAGTACCATGGAGATGCCCGAGCCCTCCGCTACTAC
  AGCCCCCCTCCCATCAACGGCCCAGGCCCGGACTTTGACCTCAGAGATGGATACCCTGACCGATACCAGC
  CCCGGGACGAGGAAGTCCAAGAGAGGTTGGACCATTTGCTGCGCTGGGTCCTGGAACACCGGAACCAGCT
  GGAGGGGGGTCCCGGCTGGCTGGCAGGGGCCACGGTGACATGGAGAGGGCACCTGACAAAATTGCTGACC
  ACGCCGTATGAGCGGCAGGAGGGCTGGCAGCTGGCAGCCTCCCGGTTCCAAGGGACACTGTACCTAAGTG
  AGGTGGAGACCCCGGCTGCTCGGGCCCAGAGGCTTGCACGGCCACCCCTCCTCCGGGAGCTCATGTACAT
  GGGCTACAAATTTGAGCAGTACATGTGCGCAGACAAACCCGGAGGCTCCCCAGACCCCTCTGGGGAAGTT
  AACACCAACGTGGCCTACTGCTCTGTGCTACGCAGCCGCCTGGGAAACCACCCTCTCCTGTTCTCCGGGG
  AGGTAGACTGCCTGAACCCCCAGGCTCCTTGCACACAGCCTCCCTCCTGCTACGTGGAGCTCAAGACATC
  CAAGGAGATGCACAGCCCTGGCCAGTGGAGGAGCTTTTACAGACACAAGTTGCTGAAATGGTGGGCTCAG
  TCGTTCCTCCCGGGGGTCCCACATGTTGTTGCCGGCTTCCGGAACCCAGAAGGTTTCGTCTGTTCCTTAA
  AGACCTTTCCTACCATGGAGATGTTTGAAAATGTCAGGAATGACCGAGAAGGCTGGAATCCCTCTGTGTG
  CATGAACTTCTGTGCTGCCTTCCTTAGCTTTGCCCAGAGCACAGTAGTCCAGGACGATCCCAGGCTTGTC
  CACCTCTTCTCCTGGGAACCTGGTGGCCCTGTCACTGTGTCTGTCCATCGTGACGCACCCTATGCCTTCC
  TGCCCTCATGGTATGTGGAGACTATGACCCAAGACCTCCCACCACTCTCAAAGACTCCCTCTCCCAAGGA
  CTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_033613
• Insert Size: 1194 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_033613.2, NP_291091.2
• RefSeq Size: 1472 bp
• RefSeq ORF: 1194 bp
• Locus ID: 112403
• UniProt ID: O70348
• Cytogenetics: 17 B1
• Gene Summary: Ribonuclease that specifically degrades pre-mRNAs with a defective 5' end cap and is part of a pre-mRNA capping quality control. Has decapping, pyrophosphohydrolase and 5'-3' exonuclease activities. Has decapping activity toward incomplete 5' end cap mRNAs such as unmethylated 5' end-capped RNA to release GpppN and 5' end monophosphate RNA. The 5' end monophosphate RNA is then degraded by the 5'-3' exoribonuclease activity, enabling this enzyme to decap and degrade incompletely capped mRNAs. Also possesses RNA 5'-pyrophosphohydrolase activity by hydrolyzing the 5' end triphosphate to release pyrophosphates.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 both encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.