Polr3g (NM_001081176) Mouse Untagged Clone

CAT#: MC207628

Polr3g (untagged) - Mouse polymerase (RNA) III (DNA directed) polypeptide G (Polr3g), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Polr3g
• Synonyms: 2310047G20Rik; AV275904; RPC7; RPC32
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC207628 representing NM_001081176
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGGCAATAAAGGAAGAGGGCGTGCTGCCTACACCTTTAATATCGAAGCTGTTGGATTCAGCAGAG
  GGGAGAAGTTACCTGATGTTGTGTTGAAGCCTCCACCCCTGTTTCCTGATACAGACTACAAGCCGGTGCC
  CCTGAAAACGGGAGAAGATGAGGATTACATGCTGGCCTTGAAGCAGGAGTTGAGGGAAACAGTGAAACGA
  TTGCCGTACTTTATTGAACCACCCGAAGAAAAACAAGATGATATTGAAAGGTATAGCAAACGATACATGA
  AGGTCTATAAAGAAGAATGGGTACCAGATTGGAGAAGACTTCCAAGAGAGATGATGCCAAGGAAAAAATG
  CAAAAAAGGGGATCCAAAATCCAAGCCGAGCAAAGCTGCAGCCAAGGCTACATCACTCATTAATTCTGCA
  GATGTGTTGAAAACGATTGAGGAACTGGAAAAGAGAGGCGAGGGTGAAAGATCGGATGAAGAAAACGAAG
  AGAAAGAAGGGAGCAAAGAGAAAGATAAAGATGATGAGGAGGATGGCGAGGAAGATGCAGAACAGGAGGA
  CTACGATGAAGAGGAACAAGAAGAGGAGAATGACTACATTAACTCCTACTTCGACAACGGCGATGATTTT
  GGTGTAGACAGCGACGACAACATGGATGAAGCCACCTACTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001081176
• Insert Size: 672 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001081176.1, NP_001074645.1
• RefSeq Size: 3060 bp
• RefSeq ORF: 672 bp
• Locus ID: 67486
• UniProt ID: Q6NXY9
• Cytogenetics: 13 C3
• Gene Summary: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. May direct with other members of the RPC3/POLR3C-RPC6/POLR3F-RPC7/POLR3G subcomplex RNA Pol III binding to the TFIIIB-DNA complex via the interactions between TFIIIB and POLR3F. May be involved either in the recruitment and stabilization of the subcomplex within RNA polymerase III, or in stimulating catalytic functions of other subunits during initiation. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts induce type I interferon and NF- Kappa-B through the RIG-I pathway.[UniProtKB/Swiss-Prot Function]