Pecam1 (BC008519) Mouse Untagged Clone

CAT#: MC206748

Pecam1 (untagged) - Mouse platelet/endothelial cell adhesion molecule 1 (cDNA clone MGC:6104 IMAGE:3592726), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Pecam1
• Synonyms: PECAM-1, Pecam
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC008519
  GCCGCCATTACCTGACAAGTACCAGAGCTGGTCTCTGCGCGCCTGGAGAGGTTGTCAGAGTTGTTTCTGC TTTTCACACAGCAGGCTTCTGGTCTGTGGCAGGCAAGAGAATTCTGCCCCTGAGGCATGGCCGGAAGACA GAACTAGCTGAGTGCTTCAGTCTGCAGAAGTCTTTCAGGATTCAGCTGAGGTGGGCCTCAGTCGGCAGAC AAGATGCTCCTGGCTCTGGGACTCACGCTGGTGCTCTATGCAAGCCTCCAGGCTGAGGAAAACTCCTTCA CCATCAACAGCATCCATATGGAAAGCCTGCCATCATGGGAGGTGATGAATGGGCAGCAACTGACCCTGGA GTGCCTTGTGGACATCAGCACCACCTCGAAAAGCAGGTCTCAGCACCGGGTGCTGTTCTATAAGGACGAT GCGATGGTGTATAACGTCACCTCCAGGGAGCACACCGAGAGCTACGTCATTCCTCAGGCTCGGGTCTTCC ACTCCGGGAAGTACAAATGCACAGTGATGCTGAACAACAAGGAAAAAACCACGATTGAGTACGAGGTGAA GGTGCATGGCGTATCCAAGCCCAAGGTGACACTGGACAAAAAGGAGGTGACAGAAGGCGGGGTCGTGACG GTCAATTGTTCCTTGCAAGAAGAAAAGCCACCGATCTTTTTTAAAATTGAAAAATTAGAAGTGGGGACAA AGTTTGTCAAGCGAAGGATAGATAAGACCTCCAACGAGAACTTTGTGCTCATGGAATTCCCCATTGAGGC GCAGGACCACGTGTTAGTGTTTCGCTGCCAAGCTGGGATCCTGTCCGGATTCAAATTGCAGGAGTCAGAA CCCATCAGGAGTGAATACGTCACCGTGCAGGAGTCCTTCTCCACTCCCAAGTTTGAAATCAAGCCCCCTG GGATGATCATAGAAGGGGACCAGCTGCACATTAGGTGCATAGTTCAAGTGACACACTTGGTCCAGGAGTT TACAGAAATTATCATCCAAAAAGACAAGGCGATTGTAGCCACCTCCAAGCAAAGCAGTGAAGCTGTCTAC TCAGTCATGGCCATGGTCGAGTACAGTGGACACTACACCTGCAAAGTGGAATCAAACCGTATCTCCAAAG CCAGTAGCATCATGGTCAACATAACAGAGCTGTTTCCCAAGCCGAAGTTAGAGTTCTCCTCCAGTCGTCT GGACCAAGGGGAGTTGTTGGACCTGTCCTGCTCCGTCTCGGGCACACCTGTAGCCAACTTCACCATCCAG AAGGAAGAGACGGTCTTGTCGCAGTATCAGAATTTCAGCAAGATCGCCGAGGAGAGTGACAGCGGGGAGT ACAGCTGTACTGCAGGCATCGGCAAAGTGGTCAAGAGAAGCGGCCTGGTACCGATCCAGGTGTGCGAAAT GCTCTCGAAGCCCAGTATTTTTCATGATGCCAAGTCTGAGATCATAAAAGGACATGCCATAGGCATCAGC TGCCAGTCCGAAAATGGAACTGCACCCATCACTTACCACCTTATGAAAGCAAAGAGTGACTTCCAGACTC TCGAGGTGACCTCCAATGACCCAGCAACATTCACAGATAAGCCCACCAGAGACATGGAATACCAGTGCAG AGCGGATAATTGCCATTCCCACCCCGCGGTGTTCAGCGAGATCCTGAGGGTCAGGGTGATAGCCCCAGTG GATGAAGTTGTGATTTCCATCCTGTCGAGTAACGAGGTACAGTCTGGAAGTGAAATGGTACTTCGGTGCT CTGTGAAAGAGGGGACGAGCCCAATCACGTTTCAGTTTTACAAAGAAAAGGAGGACAGACCCTTCCACCA AGCGGTCGTGAATGACACCCAAGCGTTTTGGCACAACAAACAAGCTAGCAAGAAGCAGGAAGGACAGTAC TACTGTACAGCCTCCAACAGAGCCAGCAGTATGAGGACCAGTCCCCGAAGCAGCACTCTTGCAGTCAGAG TCTTCCTTGCCCCATGGAAGAAAGGGCTCATTGCGGTGGTTGTCATTGGAGTGGTCATCGCCACCTTAAT AGTTGCAGCCAAATGCTACTTCCTGAGGAAAGCCAAGGCCAAACAGAAACCCGTGGAGATGTCCAGGCCA GCTGCTCCACTTCTGAACTCCAACAGCGAGAAGATTTCTGAGCCTAGTGTGGAAGCCAACAGCCATTACG GTTATGATGATGTTTCTGGAAATGATGCAGTAAAACCCATAAATCAAAATAAAGACCCCCAGAACATGGA TGTAGAATACACAGAAGTGGAAGTGTCCTCCCTTGAGCCTCACCAAGAGAACGGAAGGCTCCCTTAATGG AACTTAAGACAGTCAAGGCAGTGCACGTCCCTGGGAGGTCGTCCATGTCCCGAGAAGAGCAGCCGATTCC TGGATTGCGAGAGTCCTGTGCACGTATTTATGAGCCTGTCCCCACCGAAAGCAGTAATCCTTCAGGCCAA GCCATCCAATTTTAAACCCCGCTGCCTTGTTCATGTTGGGTATACAGAGACTCAGAGGCGCTAGTTAATG GCCAGCCTGACCTGTCTTCCTTGTGTTTCGGGGGGTGAAAGGAGGTTCCAGGAGAATAATGCCCTGGAAT ATTCTGAGGCTGAAATGTTGTGTCTCGGATAGACAGAGTACTTCTCCGTGGGTCCTCCTGCACAGAAGCC CCCTTCTGCTCTGTTGTCAATCACTGGGTTAGTTTTTTGTTTTGTTTTGTTTCTGTTGTTCTCTTCTTTC TGGGTTGATCTTAATCTTTGGAAATACACAGTGCTGCTCAGGTTTGCCTGGTTCCCCTGTGTGACCTTCC CCCCCACACACACACAAAGTCAACCAGCTGACTGGGAGCAATGCTCGTGGAATGGCCAGTCACTTGAAGA CAGACCTCAAGCCAGCAATGGCAACTGGAGCGAGCACTTAAAGCGCACCTTGATCTTCCTTTCCGTTCCA TGCCGAAGGCCCAAAGAAGAGAAATGGCTGGCTACAACAACTGTTATTATTTTTTTAATAGTAAAAGGAA ATATGTACTTTTCTCACTAATTTTTCTTTATTTATTCCCCACTAAAGAAACGGTTTCCTAAGGTCTGAGC TGTTTCCCAGGGTGGGCTAGAGTGGGTGGGCAGGAGCTGCCGACATTTTGTGTACTATACCTAACCCTCC TCGCCTTCTCCGTTTGCCTCAGCCACCCGGCTCCAGGGCCCCTGAGGGGAGTTGTATTAAATAAAATTGA TTTTTACTCTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACTAA
• Restriction Sites: RsrII-NotI
• ACCN: BC008519
• Insert Size: 2094 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC008519, AAH08519
• RefSeq Size: 3284 bp
• RefSeq ORF: 2094 bp
• Locus ID: 18613
• Cytogenetics: 11 E1
• Gene Summary: Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions (By similarity). Tyr-679 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes (By similarity). Trans-homophilic interaction may play a role in endothelial cell-cell adhesion via cell junctions (By similarity). Heterophilic interaction with CD177 plays a role in transendothelial migration of neutrophils (By similarity). Homophilic ligation of PECAM1 prevents macrophage-mediated phagocytosis of neighboring viable leukocytes by transmitting a detachment signal (By similarity). Promotes macrophage-mediated phagocytosis of apoptotic leukocytes by tethering them to the phagocytic cells; PECAM1-mediated detachment signal appears to be disabled in apoptotic leukocytes (By similarity). Modulates bradykinin receptor BDKRB2 activation (By similarity). Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in endothelial cells (By similarity). Induces susceptibility to atherosclerosis (PubMed:19048083).[UniProtKB/Swiss-Prot Function]