2410187C16Rik (BC051524) Mouse Untagged Clone

CAT#: MC206718

2410187C16Rik (untagged) - Mouse RIKEN cDNA 2410187C16 gene (cDNA clone MGC:63069 IMAGE:1514917), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: 2410187C16Rik
• Synonyms: 2410187C16Rik; AU014961; AW550036; Ntaq1
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC051524
  GGCACGAGGGCGACAATTGCGGCCAGCGGGTTGTTTAGTTGACCTGTGAGCCACAGCTTCGGGTCATGGA GGGGGACGGCCCCGCCGCCACGGCCCCGCAGTACCAGCCGGTCTGTCCTACGCGGGACGCATGTGTCTAC AACAGCTGCTATTGTGAGGAAAACATTTGGAAGCTCTGTGAGTACATCAAGACACACAACCAGTATCTGT TGGAGGAGTGCTATGCAGTCTTCATATCAAATGAGAAGAAGATGGTACCTATTTGGAAACAGCAGGCAAG ACCTGAGAATGGACCTGTGATCTGGGACTACCATGTGGTTCTGCTTCATGTGTCAAGGGAAGGACAAAGC TTCATTTATGATCTTGACACTATTTTGCCATTTCCCTGCCCTTTCGACATTTACATAGAAGATGCCCTTA AGTCTGATGATGACATTCATCTGCAGTTTAGGAGGAAATTTAGAGTGGTTCGTGCTGACTCCTACTTGAA GCACTTCGCTTCTGACCGGTCTCACATGAAAGACTCCAGTGGGAACTGGAGAGAGCCTCCCCCAGAGTAC CCCTGCATTGAAACTGGAGACTCCAAAATGAACCTGAACGACTTCATCAGCATGGACCCTGCAGTAGGAT GGGGAGCTGTCTACACGCTGCCTGAGTTTGTGCATCGGTTCAGCAGCAAAACCTACCAAGCCTGACATCC GGGCTGCAGCTAGAGAAGCTCTAGGGTCTGAGTAAGCCATCCTTCCATTTAAGAGAGAGCGTGGTACAGG TGGCCTATAGGGATATTGTTCTCACTAAATGTGAGTGGAAACATTAGTGAACACAGATGAAAATGAATGA CTAAGTCCCCCACCCCCTTTTTTTAGGCTGGGGATCTATCCCAGAGTTCTATCTCCAACCCTAAACAGTC AGACCCCAGCCTCAGACATAGATATGCCACATTCGGTATATGGTTGCTAAAATGTTTCCATGGCTTGCTT ACTTCGGTGTGTGATGACGTAGGCCAGCAATTGCTTTTGTAAAACTAGAGGTGTGGACCTGGAGTCGGCT CCTTTTGTCTGCTTCCTCTGCTTCCTGTCTGGCTAGACTCTAAACTCCTTGAGCACAGGTTTGTGGCCTG TGGCTCTAAGACTCTGTTGCCCATTAGAGGTCCCGGTAAAATGTTTCTAGAGTCAGCACTGCTGCTTGGT AAGGGACAGTATTGTGAGCTGAATCAGCTGTAAGTATTAGTCTTTTTGGACTACGATGTTTAAAAAAGTT GCAGATATCTTGTCTGAGTGCTATTTGACCCACTTATATATGTAGTTTTAAATAAAATTGACTTAAAATT AAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: EcoRI-NotI
• ACCN: BC051524
• Insert Size: 630 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC051524, AAH51524
• RefSeq Size: 1354 bp
• RefSeq ORF: 630 bp
• Locus ID: 76773
• Cytogenetics: 15
• Gene Summary: Mediates the side-chain deamidation of N-terminal glutamine residues to glutamate, an important step in N-end rule pathway of protein degradation. Conversion of the resulting N-terminal glutamine to glutamate renders the protein susceptible to arginylation, polyubiquitination and degradation as specified by the N-end rule. Does not act on substrates with internal or C-terminal glutamine and does not act on non-glutamine residues in any position. Does not deaminate acetylated N-terminal glutamine. With the exception of proline, all tested second-position residues on substrate peptides do not greatly influence the activity. In contrast, a proline at position 2, virtually abolishes deamidation of N-terminal glutamine.[UniProtKB/Swiss-Prot Function]