Sart3 (BC057156) Mouse Untagged Clone

CAT#: MC206673

Sart3 (untagged) - Mouse squamous cell carcinoma antigen recognized by T-cells 3 (cDNA clone MGC:68131 IMAGE:5347469), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Sart3
• Synonyms: AU045857; mKIAA0156
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC057156
  GCAAGATGGCGACGACGGCCGCATCTTCGGCGTCCGAGCCGGAGGTTGAGCCCCAGGCCGGGCCTGAGGC CGAGGGAGAAGAGGATGAGGCGAAGCCGGCCGGTGTGCAGCGGAAGGTGCTGTCCGGCGCTGTGGCCGCG GAGGCGGCGGAGGCCAAGGGCCCCGGATGGGACCTGCAGCGGGAAGGCGCGAGCGGGAGCGATGGGGATG AGGAGGACGCCATGGCTTCCTCCGCCGAGAGCTCCGCCGGGGAGGACGAGTGGGAGTACGACGAGGAGGA GGAGAAGAACCAGCTGGAGATCGAGCGGCTGGAGGAGCAGCTGTCCATCAATGGCTATGATTACAACTGC CACGTGGAGCTCATCAGGCTGCTGCGGCTGGAAGGCGAGCTCAGCAGAGTGAGGGCGGCCCGCCAGAAGA TGAGTGAGCTCTTCCCCCTGACCGAAGAGCTCTGGCTGGAGTGGCTCCACGATGAGATCAGCATGGCCAT GGACGGCCTGGACCGCGAGCACGTGTACGAGCTCTTTGAGAGAGCCGTGAAGGACTACATCTGTCCAAAC ATTTGGCTAGAGTATGGCCAGTACTCAGTTGGTGGCATTGGTCAGAAAGGTGGCCTTGAGAAGGTTCGCT CTGTCTTTGAAAGAGCCCTGTCCTCTGTTGGCCTGCACATGACGAAAGGCCTGGCCATCTGGGAGGCCTA CCGAGAGTTTGAAAGCGCCATCGTGGAGGCTGCTCGGCTGGAGAAAGTGCACAGTCTCTTCCGGCGACAG CTGGCGATCCCACTGTACGAGATGGAGGCCACCTTTGCAGAGTATGAAGAATGGTCAGAGGAGCCCATGC CGGAGTCTGTACTTCAGAGCTATCAGAAAGCGCTGGGGCAGCTAGAGAAGTACAAGCCTTACGAGGAAGC GCTGCTGCAGGCAGAGGCCCCTCGGCTGGCGGAATACCAAGCTTACATCGACTTCGAGATGAAAATCGGG GATCCTGCCCGTATTCAGTTGATCTTTGAGCGTGCCCTGGTGGAGAACTGCCTGGTTCCAGACTTATGGA TCCGCTACAGTCAGTACCTAGATCGACAGCTGAAAGTTAAGGACTTGGTTTTATCTGTACACAGCCGTGC TGTGCGGAACTGCCCATGGACAGTTGCCCTGTGGAGTCGGTACCTTCTGGCCATGGAGCGACATGGACTG GACCATCAAACGATTTCTGCGACCTTCGAGAACGCTCTGAGTGCCGGCTTCATCCAGGCCACTGACTATG TGGAGATCTGGCAGGTGTACCTCGACTACCTGAGGAGAAGGGTTGACTTCAGACAGGACTCTAGCAAGGA GCTGGAAGAGCTGCGGTCCATGTTCACGCGAGCTCTGGAGTACCTGCAGCAGGAGGTTGAGGAGCGTTTC AGCGAGAGTGGGGATCCAAGCTGCCTGATCATGCAGAGCTGGGCTCGGGTTGAGGCTCGCCTGTGCAATA ACATGCAGAAAGCCCGAGAGCTCTGGGACAGCATCATGACCAGAGGGAATGCCAAGTACGCCAACATGTG GCTGGAGTATTACAACCTGGAACGGGCACACGGTGACACACAACACTGTCGGAAGGCTCTGCACCGAGCT GTCCAGTGCACGAGTGACTACCCTGAGCACGTCTGTGAAGTGTTGCTCACCATGGAGAGGACAGAAGGGA CCTTAGAAGATTGGGATCTAGCCATTCAGAAAACGGAGACGCGCTTGGCTCGTGTGAATGAGCAGAGAAT GAAGGCCGCAGAGAAGGAAGCAGCTCTTGTGCAGCAGGAAGAAGAAAAGGCCGAGCAGCGGAAGAAGGTG CGGGCGGAGAAGAAAGCCCTGAAAAAGAAGAAGAAAACGCGAGGTGCCGACAAGCGCAGGGAGGACGAGG ACGAGGAGAACGAGTGGGGCGAAGAGGAGGAAGAGCAGCCTTCCAAACGCAGAAGGACGGAGAACAGTCT GGCCTCTGGAGAGGCTTCGGCTATGAAGGAAGAAACAGAGCTCTCCGGGAAATGCTTAACGATAGATGTT GGTCCTCCTTCCAAGCAGAAAGAGAAGGCAGCCTCCCTTAAGCGGGACATGCCCAAGGTGGCTCACGACA GCAGTAAGGACAGTGTCACCGTGTTTGTCAGCAACCTGCCCTACAGCATAGAAGAGCCCGAGGTGAAGCT CAGGCCGCTCTTTGAGGTCTGTGGGGAGGTGGTCCAGATCAGGCCAATTTTCAGCAACCGCGGGGACTTC CGGGGCTACTGCTATGTGGAGTTTGGAGAGGAGAAGTCAGCCCAGCAGGCCCTGGAGCTGGACAGGAAGA TTGTGGAGGGCAGGCCGATGTTTGTGTCCCCCTGTGTGGATAAGAGCAAAAACCCTGATTTTAAGGTGTT CAGATACAGTACCACCCTGGAGAAACACAAACTCTTCATCTCTGGCCTGCCCTTTTCCTGCACCAAAGAG GAGCTCGAGGACATTTGTAAGGCCCACGGCACCGTCAAGGACCTCAGGCTGGTCACTAACAGGGCTGGCA AGCCGAAGGGCCTGGCGTATGTGGAGTATGAAAACGAGTCCCAGGCGTCCCAGGCAGTGATGAAGATGGA CGGCATGACCATCAGAGAGAATGTCATCAAGGTGGCAATCAGCAATCCCCCTCAGCGAAAAGTCCCAGAG AAGCCAGAAGTGAGGACAGCACCAGGGGCCCCCATGCTCCCCCGGCAGATGTATGGCGCGCGCGGGAAGG GACGGACCCAGCTCTCTCTTCTTCCTCGAGCTCTGCAGCGCCAGGGTGCTGCTCCTCAGGCTGAGAACGG CCCAGCTCCGGGGCCCGCGGTCGCCCCGTCTGTGGCCACAGAGGCTCCTAAGATGTCCAATGCTGATTTT GCGAAGTTGCTTCTGAGAAAGTGAGCAGGACTCTGAGATGGAGATGCCTTACCTGTCCTCAAGCTGGCCG GGCTGGCCACCACGGGCCCTGGAGACGGAAGGGCTGGGCACTTGCCTGCGCTCCCACAGATTCTCCTCTG GTGTGGATGGGAAGGGAGAGCCTATGGTGAACATGGCGGTGAGGAGTGTTCCCTCACATTGAGGGCGGAG GCCAACCGCTCTACAGGCTGTCCCAAGGTACGTTAGTGTCCTAACAAGGAGGGACCCAGCTTTCGAGGCC CACTTGTCCTGATGCTTTCACCGCCTCTGGCCCCTTTTCTACGAACCCCCTCCCCCAGCCCTGCACAGCA CGTGTGCCCATCACTCTGTAAGTGTGGAAGATGGAATGGGAGAGCTTGTCACTCATCAGAATGGCCTGTC GAGAAGTGCGGGACGTCACAGAAGACACGTGTGATGGGCTTTTGTCCAAAGAGGCTATGAGTTTTTCTAT TATGTATTTCTAATTGACACTGTTTAATGTTCCCTAAAAGTGGAATGTCCGCGACCTTTGTTCTTAGGAA TTGTGTGCCAGCCACTCTGGACCACTTTCTTTGCCTGATGACTGGAGCCCTAAGCCCTCTGTTCAGATGC TCACTTCGAAATGCCATGTCTAGTACAAAGTTGAGTCTCCCCATTGAGTTTTGTTTATTAAACTGAAGTT CTTACAGAAAAAAAAAAAAAAAAAAA
• Restriction Sites: EcoRI-NotI
• ACCN: BC057156
• Insert Size: 2889 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC057156, AAH57156
• RefSeq Size: 3596 bp
• RefSeq ORF: 2889 bp
• Locus ID: 53890
• Cytogenetics: 5 F
• Gene Summary: U6 snRNP-binding protein that functions as a recycling factor of the splicing machinery. Promotes the initial reassembly of U4 and U6 snRNPs following their ejection from the spliceosome during its maturation. Also binds U6atac snRNPs and may function as a recycling factor for U4atac/U6atac spliceosomal snRNP, an initial step in the assembly of U12-type spliceosomal complex. The U12-type spliceosomal complex plays a role in the splicing of introns with non-canonical splice sites. May also function as a substrate-targeting factor for deubiquitinases like USP4 and USP15. Recruits USP4 to ubiquitinated PRPF3 within the U4/U5/U6 tri-snRNP complex, promoting PRPF3 deubiquitination and thereby regulating the spliceosome U4/U5/U6 tri-snRNP spliceosomal complex disassembly. May also recruit the deubiquitinase USP15 to histone H2B and mediate histone deubiquitination, thereby regulating gene expression and/or DNA repair (By similarity). May play a role in hematopoiesis probably through transcription regulation of specific genes including MYC (PubMed:21447833).[UniProtKB/Swiss-Prot Function]