Casq1 (BC064057) Mouse Untagged Clone

CAT#: MC206664

Casq1 (untagged) - Mouse calsequestrin 1 (cDNA clone MGC:73607 IMAGE:876739), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Casq1
• Synonyms: CSQ, CSQ1, CSQ-1
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC064057
  CCTCTGTCCACTGCTCTGGCCCACTCTCTACCCTACCCCCACCTGGAGCCCCTAACTCAGGATTCTGTAT CCAAGGGGCACCTCTCCCTATTTCAGCTAACCTCCTCTGGACCAGGAGAGCAGGCCCAGATTGTACTACC TCCATGAGAGCTACCGACAGGATGGGGGCCAGAGCAGTGTCCGAGCTGCGGCTGGCACTGCTGTTTGTAC TGGTGCTAGGGACGCCCAGGTTAGGGGTCCAGGGGGAAGATGGGTTGGACTTCCCTGAGTACGACGGTGT GGACCGTGTGATCAATGTGAACGCCAAGAACTACAAGAACGTGTTTAAGAAGTATGAGGTGCTGGCCCTC CTCTACCATGAGCCCCCCGAGGACGACAAGGCCTCGCAGAGACAATTTGAGATGGAGGAGCTAATCCTGG AGTTAGCAGCCCAAGTCTTAGAAGACAAGGGTGTTGGCTTTGGCCTGGTGGACTCAGAGAAGGATGCAGC TGTGGCCAAGAAACTAGGACTAACTGAAGAAGACAGCGTTTATGTGTTCAAAGGAGATGAAGTCATTGAA TATGACGGCGAGTTTTCTGCAGACACTCTGGTGGAGTTTCTGCTTGATGTCCTAGAAGACCCTGTAGAGT TGATTGAAGGTGAACGAGAGCTGCAGGCATTTGAGAATATTGAAGATGAAATCAAACTCATTGGCTACTT CAAGAGCAAAGACTCAGAGCATTACAAAGCCTACGAGGACGCAGCTGAAGAGTTCCATCCCTACATCCCT TTCTTCGCTACCTTCGACAGCAAGGTGGCAAAGAAGCTGACTCTGAAGTTGAATGAGATTGATTTCTACG AGGCCTTCATGGAAGAGCCTATGACCATCCCAGACAAGCCCAACAGTGAAGAGGAGATTGTGAGCTTCGT GGAGGAGCACAGGATATCAACCCTGAGGAAACTGAAGCCTGAGAGTATGTACGAGACTTGGGAGGATGAC CTGGATGGAATCCACATTGTCGCCTTTGCAGAGGAAGCAGATCCTGATGGCTATGAGTTCTTAGAGACTC TCAAGGCTGTGGCCCAAGACAACACTGAGAACCCCGACCTCAGTATCATCTGGATTGATCCTGATGACTT CCCGCTGCTGGTCCCGTACTGGGAGAAGACCTTTGACATTGACCTGTCAGCTCCACAAATAGGAGTTGTC AATGTTACAGACGCGGACAGCATATGGATGGAGATGGATAACGAGGAGGACCTGCCTTCTGCTGATGAGC TGGAGGACTGGCTGGAGGACGTGCTGGAGGGCGAGATCAACACAGAGGATGACGACGACGATGACGACGA TGACGATGATGACGATGATGATGACGACTAGATGACTAGTGGCCGTGGCAGCCGCGTACCAGCCCCACTT GCTCCTTACTTGTTGTACCTTTCTGCCATCTGTGCCCTTCCCCGGGCTCCTTGGGACACTAGGTCATTCT CTACTACAGGGCCAACTGTGGTCTGCATACAGGGTGCTTGAGGTCCTGGTCTCCCTCTCCGAGGAGAAAA GGAGCTGCTTTTTCTTAGATCCCAGCCCAGTTCTCTCATCTGACTTGCTTCTTCCCCCAGACCTTATCTG TTTCTCTTCCCGCCACTCCCCATACTTAACGGTTTGCAAACTCTTCTCTATCCCATTTCTCAAATCTTAA ACCTCTCTCACTGTCCTGACCCTGGCCAGGAGGAAGGGAGGTACTGTGTTAGGGGCTATAATCTTAGCCA TCTCCTGTTAATGTATTTGGGTCAAATGCAAGGCCTTAATAAAGAAATCTGGGGCAGAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: EcoRI-NotI
• ACCN: BC064057
• Insert Size: 1200 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC064057, AAH64057
• RefSeq Size: 1877 bp
• RefSeq ORF: 1200 bp
• Locus ID: 12372
• Cytogenetics: 1 79.54 cM
• Gene Summary: Calsequestrin is a high-capacity, moderate affinity, calcium-binding protein and thus acts as an internal calcium store in muscle. Calcium ions are bound by clusters of acidic residues at the protein surface, often at the interface between subunits. Can bind around 80 Ca(2+) ions (By similarity). Regulates the release of lumenal Ca(2+) via the calcium release channel RYR1; this plays an important role in triggering muscle contraction. Negatively regulates store-operated Ca(2+) entry (SOCE) activity (By similarity).[UniProtKB/Swiss-Prot Function]