Atf7ip (BC052460) Mouse Untagged Clone

CAT#: MC206147

Atf7ip (untagged) - Mouse activating transcription factor 7 interacting protein (cDNA clone MGC:63433 IMAGE:6834550), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Atf7ip
• Synonyms: 2610204M12Rik; AM; Mcaf1
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC052460
  GCGGTGGCGGCGAGTGGAGCGCACGGCTGAGACGGCAGCGTCCGGGACGGCTGCACGGGACGGCTGCGTA GGAAGGAACTCGGGTCCCAGGCTCTCACCTCCCGCCCCCGAAGATTCAGAATGGATAGTGTAGAAGAACC TCAGAAAAAAGTCTTCAAGGCTCGGAAAACAATGAGAGCGAGCGATCGCCAGCAGCTCGATGCTGTGCAC AGAGTCAAGGGGGAGCTGCTGAGAGCCGACGGGAAGCTGCTGAACGGGAGCCATGAGAATGGAGACTTGG ATCCCACTTCACCCTTGGAAAACACAGATTGTATTCAAGATCGAGAAGAAGTGAATGGTATTGATGGGAT TTGTTTTCAGTCAGAAGAAAGTACAACGGAATGGAAAGAGACCCCTTGTATGCCCAATGTTGCTGTAAAA AACAAACAAGAAGATTTGAATAGTGAAGCTTTGTCCCCCAGTATAACTTGTGATCTTTCTTCTAGAGTAA CCACTGAACCAGGTTCTGGTAGTCCAGCCTCTGATAACCCAGGCTGTGGTACCCCAGTCTCTGATAACCC AGCCTCCGATAACCCAGCCTCTGACAACCCAGCCTCTGACAACCCAGACTCTGGTGATCTGGCTGCAGGA GAGCTGGCCACTACAGTACAGGCTACTGGTGACTCTGCCTGTGAGGAGCCTCCCTCCAGTGATCCCTCTT CCAGTGATCCCACTTCCAGTGAGCCCTCTTCCAGTGAGCCCACCTGTAGTGAGCCCATCTCTGGAGATCC TGTTTCTGAAGAAGCCGCCTCTCATGATCTTGTCTCTGGTGATTCCACTTGTAGCGAGCCAGTTTCTGGT GAGCCGGTGTCTCATGAAGCAGCTTCGAGTGAGCCAGCTACAAGTGAGCCAGCTTCAGATGAACCGGTCG CAAGGGTCGTGGCGGCCTGCGAACTGGCTCCTGGGGAGTCAGCCTTGGATGATTGTGCCCCCTCCGGTGA TTCACAGTCTGATGAGCCACCTTCTAGTGAGGACTCCCTTCCTAGGTCTGTCTGCAGCGGACTGGCTTCT GGTGAGCTGACTCCTGGGGAGCTCTCTGTGGAACCAGCCACCGACACTGTTAAACCAAGCTCATCGGCAG TTTGTGAAGCAGGTCCTGACCCTGACAAGACAGAGCCGAGTAGCAATAACAGTGATGATTGTCCAGGCAA GAGTGAAGACGACGAACACTTAGACCAAATTCAAAGTAAAGACTCCTGTGATGAGGGAAATAAAGTGAAC AGTAATGTCGTCGAGAAGGAAGAACCTCTCGAAACACACAGTGCAATTATTTGTTCTGATCTACCTCCAG AAAACACAACGAAGATAGCGGAGGATCCCATCGCTGAGCCTGCTCTCGAGGAAGAGGCCATCTCCAGCAG CATGGAAGTTGACCAAAGCGAAAAGGATGAGCATAAAAGTCCTGCAGAACCTGTAGCCGCTGTCAGTGAG GACCCCGCCGAGGAGGACAAGGAGGATACTGTCGTAGACAACACGGACTCCATGGAAACAGATGAGATCA TTCCGATTTTGGAAAAACTCGCACCCACCGAGGATGAACTCAGTTGCTTTTCAAAAGCTTCTCTCCTTCC AGTCGAAACAAGTCAAGATTTGGAGGATAAAATGGAGGGCTCTTTCGGCTCACCCTCGAAACAAGAAAGT AGTGAGAATTTGCCAAAAGAAGCCTTTTTAGTCCTTTCTGATGAAGAGGATCTTTCGTGTGGAAAAGATG AGTCCGAAGCTGTAGCGCAGAGTAAGATGAGCACCCCAGAGGGAGAAAAGAGTGAGAAGGACGGCAAAGC TGAGGAAGAGGAGCGAGTTCCAGCCGAGGAGCAGCCACCTGTGAGAAATGAGTTTTCTAGACGAAAACGT TCTAAGTCAGAAGACATGGACAGTGTAGAGTCCAAACGCCGGCGATATATGGATGAAGAATACGAGGCTG AGTTTCAAGTAAAGATCACAGCTAAAGGTGACATCAACCAGAAGCTTCAAAAGGTCATACAGTGGTTGCT ACAAGAAAAACTGTGTGCCCTACAGTGTGCTGTGTTTGATAAGACGTTGGCAGAATTAAAAACTCGAGTG GAGAAGATAGAGTGCAACAAGAGGCATAAGGCAGTGCTCACCGAGCTGCAGGCCAAGATAGCCAGGTTAA CCAAACGTTTTGGAGCAGCCAAAGATGATCTTAAGAAAAGACAAGAAAGCCCTCCAAACCCACCCATATC ACCAGGAAAGCCTGCCAATGACACCAACAGCAATAATAATATGACCTACAGAAATGCAGGCACAGTGCGA CAACTGCTGGAGTCCAAAAGAAATGTTAGCGAGGGTCCACCACCATCCTTTCAAACTCCTGTGAATACAG TGTCTTCAGCCAGTCACGCCACTTCTACAGCAGTTGTCAGTAGTCAGCCTAAACTGCAGACCTCAGCAAC CTCGGGCTCTCTGCCGGCGGCACCTCTTCTTCCTGCGCCCAGCACAGCCACCGTAGTTGCCACTACTCAG GTGCCTAGTGGGACCCCTCAGCCTACAATCTCTCTGCAGCCCTTGCCAGTGATCCTGCATGTGCCCGTTG CCGTGACCTCTCAGCCTCAGCTCCTGCAAAGTCATCCAGGGACTTTAGTGACCAATCAACCATCTGGCAA CGTGGAGTTCATATCTGTGCAAAGCCAACCTACAGTGAGTGGTCTTACCAAAAATCCAGTATCCTTGCCA CCCTTGCCAAACCCCACTAAACCAAACATTCCGTCAGTGCCCAGTCCTAGTAGTATTCAGAGGAACTCTT CAACCACCGCCGCACCGTTGGGAACAACCCTTGCTGTCCAGGCCGTTCCAACCGCACATTCTATTGTGCA GGCCACAAGGACTTCTCTACCCACAGTAGGCCCGTCAGGACTCTATAGCTCTTCAAGCAGTCGAGGCCCA ATACAGATGAAAATCCCAATATCCACTTTTAGCCCTCCATCTTCAGCAGAACAAAATAGCTCTGCCACCC CAAGAATTGTTACAGAAAACCAGACAAACAAAACAGTAGATTCTTCTATTAATAAGAAAGCAGCCGATAG CACATCACAGAGTGGAAAAGCTTCTAGCAGTGACTCAAGTGGTGTCATTGATCTCACGATGGACGATGAA GAGAGCGGAACTACACAAGACCCCAAGAAGATAAGTCCTCCTTCTTCATCAACTGTGAGCACTTCTCAGC CCATGTCTCGACCGTTGCAGCCCATTCTGCCAGCACCACCTCTTCAGCCATCAGGCGTGCCCACAAGTGG ACCATCTCAGGCAACTATCCATGTTCTGCCTACAGCTCCAACCACCGTGAATGTAACACATCGTCCAGTA ACTCAGGTGACCACAAGACTCCCTGTACCGAGAGCTCCTGCAAACCACCAAGTGGTTTATACAACTCTCC CAGCGCCAACCACTCAGGCTCCCCTACGAGGAACTGTTATGCAGGCTCCTGCCGTTCGCCAGGTCAATCC TCAAAATAGTGTTACAGTTCGAGTGCCTCAGACAACCACATATGTTGTAAACAATGGACTGACTCTAGGC TCAGCAGGGCCTCAGCTCACGGTGCATCACAGGCCCCCACAAGTGCACAATGAGCCCCCACGCCCTCTGC ATCCAGCGCCCTTACCAGAAGCCCCACAGCCCCAGCGTCTGCCCCCGGAAGCTGCCAGCACTTCTCTGCC TCAGAAACCACACTTGAAGTTAGCACGAGTTCAGAGTCAGAATGGCATTGTTCTGTCGTGGAGCGTCCTG GAAGTGGACCGAAGCTGTGCTACTGTTGACAGCTACCATCTCTATGCTTACCACGAGGAGCCCAGTGCCA CCGTGCCCTCACAGTGGAAGAAGATCGGGGAAGTCAAGGCCCTGCCCTTGCCCATGGCTTGCACTCTCAC GCAGTTTGTGTCTGGCAGCAAGTACTACTTTGCAGTGCGCGCCAAGGACATTTATGGCCGCTTTGGGCCT TTCTGTGACCCACAGTCCACGGACGTGATCTCTTCCTCCCAGAACAGTTAGACCTTGGAGTCGTTTAGCT TCTACTTTTGAAAGTTTCACATCTTGGGCTTGTTTTTAATCTTGTGCATGAAATCCACTGTAAAAATCCA CCTTGTGCAAGATCTCCCGGACATGTGACTGTATACACTACGTTTGTTTATAACCGGAAGCAAAATAAAT TCAGCCCATAAAGCAAGCCTTCCCTGAACAATTCAAACTTCGGGGTTTTGAAATGTATATTTATTCCTTG CTTTTTGGTTTTGAGTTTTGAAACTGGGGAATATGTATGGGTGTTTGTGTGTTTTCTTCTTTTGTTTTGA ATTTTTAAATTTCTAGTTGTGTGTGTATCACAGTGCGATCTCCTGTTTTCCTCTCAAATTTACCTCTCTT CCAGAAGGAAGTATATTGCTGAGCGAATCTGAGGTGCAGAGCAGGTGTGATTCTGCCTCCAGAGGAGGCT AGAAGGTCAAAGAATCTAAATAAAACCCGAAGGAAGGAAGGAAGGAAAAAAAAAAAAAAAAAA
• Restriction Sites: AscI-NotI
• ACCN: BC052460
• Insert Size: 3921 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC052460, AAH52460
• RefSeq Size: 4543 bp
• RefSeq ORF: 3921 bp
• Locus ID: 54343
• Cytogenetics: 6 G1
• Gene Summary: Recruiter that couples transcriptional factors to general transcription apparatus and thereby modulates transcription regulation and chromatin formation. Can both act as an activator or a repressor depending on the context (PubMed:10777215). Required for HUSH-mediated heterochromatin formation and gene silencing (By similarity). Mediates MBD1-dependent transcriptional repression, probably by recruiting complexes containing SETDB1. Stabilizes SETDB1, is required to stimulate histone methyltransferase activity of SETDB1 and facilitates the conversion of dimethylated to trimethylated H3 'Lys-9' (H3K9me3). The complex formed with MBD1 and SETDB1 represses transcription and couples DNA methylation and histone H3 'Lys-9' trimethylation (H3K9me3) (PubMed:14536086). Facilitates telomerase TERT and TERC gene expression by SP1 in cancer cells (By similarity).[UniProtKB/Swiss-Prot Function]