Ddx42 (BC043036) Mouse Untagged Clone

CAT#: MC206123

Ddx42 (untagged) - Mouse DEAD (Asp-Glu-Ala-Asp) box polypeptide 42 (cDNA clone MGC:57931 IMAGE:5698965), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ddx42
• Synonyms: RNAHP, RHELP, SF3b125
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC043036
  GCATCATGAACTGGAATAAAGGCGGCCCTGGAACCAAGCGGGGATTTGGTTTTGGAGGTTTTGCTATCAG TGCTGGGAAGAAGGAGGAAGCCAAACTGCCACAGCAGTCCCATAGTGCCTTTGGGGCAGCCAGCTCTTCT TCTGGATTTGGAAAGTCTGCTCCACCACAGCTTCCTTCTTTCTACAAAATTGGATCTAAACGGGCCAACT TTGATGAAGAAAATGCGTATTTTGAAGATGAAGAAGAAGACTCCAGCAATGTAGATTTACCTTACATTCC TGCTGAAAACTCACCTACCCGCCAGCAGTTCCATTCCAAGCCCGCAGACTCTGACAGTGATGATGACCCC TTAGAGGCATTCATGGCTGAAGTGGAGGATCAGGCTGCTAGAGACATGAAGAGACTTGAAGAAAAGGACA AGGAAAGAAAAAACGTCAAGGGTATTCGAGATGACATTGAAGAGGAAGATGACCAAGAAGCTTATTTTCG ATACATGGCAGAGAACCCAACTGCTGGGGTGGTTCAGGAGGAAGAAGAGGACAATTTGGAGTATGACAGT GATGGAAATCCAATTGCACCTTCCAAAAAAATTATCGATCCCCTTCCTCCCATTGATCATTCGGAGATTG ACTATCCACCATTTGAAAAAAATTTTTACAATGAACATGAGGAGATAACCAACCTCACTCCACAGCAGTT GATAGACCTGCGACATAAGCTCAACCTTCGGGTCTCTGGTGCTGCACCTCCCAGACCAGGAAGTAGCTTT GCTCATTTTGGGTTTGATGAACAACTTATGCACCAGATTAGGAAATCTGAGTACACACAGCCGACCCCAA TACAGTGTCAGGGTGTACCTGTAGCACTAAGTGGGAGAGACATGATTGGTATTGCTAAAACAGGCAGTGG GAAAACTGCAGCCTTTATTTGGCCCATGTTGATTCATATAATGGATCAGAAGGAATTGGAACCAGGTGAT GGACCAATTGCAGTTATTGTGTGTCCTACTAGGGAGCTTTGTCAACAGATCCACGCAGAGTGTAAGCGGT TTGGGAAAGCGTATAATCTTCGATCAGTGGCCGTGTATGGAGGAGGGAGCATGTGGGAGCAGGCCAAGGC CCTTCAGGAAGGGGCAGAGATTGTTGTATGTACCCCAGGTCGACTGATTGATCATGTGAAGAAAAAAGCT ACTAATCTTCAAAGAGTCTCTTACCTTGTGTTTGATGAAGCCGATCGAATGTTTGACATGGGATTTGAGT ATCAGGTTCGGTCCATAGCAAGTCATGTTCGTCCTGACAGACAAACTCTCTTATTCAGTGCAACTTTTCG AAAGAAGATTGAAAAGTTGGCCAGAGACATCTTGATTGACCCTATTCGAGTGGTGCAGGGAGACATTGGA GAGGCGAATGAAGATGTGACACAGATTGTGGAGATTCTCCATTCCGGACCCAGTAAATGGAACTGGCTGA CTCGGCGTCTGGTGGAGTTTACTTCCTCGGGGAGTGTGCTCTTGTTTGTTACTAAGAAAGCCAATGCTGA AGAGCTAGCCAGTAACCTGAAGCAGGAGGGTCATAATCTTGGTCTGCTCCATGGGGACATGGATCAGAGC GAAAGAAACAAGGTTATTTCAGATTTTAAGAAAAAGGACATCCCCGTCCTGGTGGCCACTGATGTTGCAG CCCGTGGTCTGGATATTCCTTCCATTAAGACAGTCATTAACTATGATGTAGCACGGGACATTGATACGCA CACTCACAGGATTGGCCGGACAGGAAGAGCTGGTGAGAAGGGTGTAGCCTATACCTTATTGACTCCAAAG GACAGCAATTTTGCTGGTGACCTTGTACGGAACCTGGAAGGAGCCAACCAACATGTTTCTAAGGAGCTCT TAGATCTTGCAATGCAGAATGCCTGGTTTCGGAAATCTCGCTTCAAAGGAGGGAAAGGCAAAAAGCTGAA CATTGGTGGAGGAGGCTTGGGCTATAGAGAGCGGCCTGGGCTGGGCTCTGAGAATTCGGACCGAGGAAAT AATAACAATGTAATGAGCAATTATGAAGCCTACAAGCCCTCCACAGGAGCCATGGGAGATAGGCTCACAG CAATGAAAGCAGCTTTTCAGTCCCAGTACAAGAGTCACTTTGTTGCAGCCAGTTTAAGTAATCAGAAGGC CGGAACCTCTTCTGCAGGGGCAAGTGGATGGACTAGTGCAGGAAGCTTAAATTCTGTTCCAACTAATTCA GCACAGCAGGGCCATAATAGTCCTGACAACCCCATGACCAGTTCTACCAAGAACATCCCAGGCTTCAACA ACTCTGGGAATATCAGTAGTGCCCCAGTGACCTACCCTTCTATTGGAGCCCAAGGAGTCAACAACACAGC TTCAGGGAATAACAGCCGTGAAGGGATTGGGGGTGGCAATGGGAAGAGAGAGAGATATACTGAGAACCGG GGTGGTAGCCGTCATAGCCATGGAGATGGTGGCAATCGGCATGGAGATGGTGGGCGCCATGGAGATGGAT ACCGTTATCCAGAAAGTGGTAGCCGTCATACTGATGGCCACCGACATGGAGAGACCAGGCATGGAGGAAG TGCCGGCAGACACGGAGAGAGCCGGGGTGCAAATGATGGCAGGAATGGAGAAAGCAGGAAAGAAGGTTTT AATCGTGAAAACAAGATGGACCCCAAGGTGGATAGCAGCAGGATGGACAAAGTGGACAGCAAGACAGATA AGACACCTGATGGCTTTGCTGTTCCAGAGCCACCCAAAAGAAAGAAAAGCCGATGGGATAGTTAGAGGGT ATGTGAAAATCAGCGTCGTTAGTCGTCTTTAATTTTATTTTTAGAAAGATTTTGGTAATGAGGCGTCTCA GGGCTGGGTTGGGGTCCAAGATATACAGACCCCCTGCCCTTGGTGGAGAACTGGAGAGTGGATTGTACCT TCATCTGGTGTTCATTCAGAGGTTTCCTTGGGAACCTGAGCTGGCCCTGGAAAGCAGAGAGCTGGGAAGC TTTTGTTTGTTGTGGGTGAGTTTTAAGGAGGGTAAGTAGAATACTTAGGGAGAAAGGGCCTTTTAGGGCA CAAAAACTCACTCTAGGTTTATAATAGCTTATATTTTTTACTAAAATGTCACCTTATAAACATCTATAAA TTGGACTTTTTTTCTTAGCTGTGGCCAGGCAGTCCCAACTTCTGCCCTGTTAGGGATGCTTGGGGAGCAG TGGTGCTTTCAGATGTTTGCAGGGAAGATGCCTGAGCGCTCAGGTTCCCCAGACTGTTTCCAGCCCTGAG ACACGTAGGAAAGACAGCCTTCCAGACACAGGCTTTGAAATCCTAAGCCATTAGGACTGAAGGAATGATC ATCTTGGAAGGATTCCAGGATAATCCAGGCCTGGAGTACTGCTAAATTTACCATTTAAAACCAACCCCAA CAGTAGCATTCAAAGTGTCTATTTGTTTTGTTTTGTTTTTTTAAACTTGCCCTAATAGTAGAAAAAAGTC TTCTGCTGAAATGATTTTGATTTTTGTTCTATCTTGTTTATAAAAGAAAAATATACAAACTTTGAAAAAA AAAAAAAAAAAA
• Restriction Sites: AscI-NotI
• ACCN: BC043036
• Insert Size: 2433 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC043036, AAH43036
• RefSeq Size: 3582 bp
• RefSeq ORF: 2433 bp
• Locus ID: 72047
• Cytogenetics: 11 E1
• Gene Summary: ATP-dependent RNA helicase. Binds to partially double-stranded RNAs (dsRNAs) in order to unwind RNA secondary structures. Unwinding is promoted in the presence of single-strand binding proteins. Mediates also RNA duplex formation thereby displacing the single-strand RNA binding protein. ATP and ADP modulate its activity: ATP binding and hydrolysis by DDX42 triggers RNA strand separation, whereas the ADP-bound form of the protein triggers annealing of complementary RNA strands. Involved in the survival of cells by interacting with TP53BP2 and thereby counteracting the apoptosis-stimulating activity of TP53BP2. Relocalizes TP53BP2 to the cytoplasm (By similarity).[UniProtKB/Swiss-Prot Function]