Kcnd2 (BC079667) Mouse Untagged Clone

CAT#: MC206092

Kcnd2 (untagged) - Mouse potassium voltage-gated channel, Shal-related family, member 2 (cDNA clone MGC:90664 IMAGE:30356567), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Kcnd2
• Synonyms: Kv4.2, mKIAA1044
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC079667
  TGTGAGGGAAACCTGACCTTCTTGAGTACTGTGACTTGACCTGCGCCACCTTGGAATAATCGTGTTCCTG TGAGCAGTGTCCCCCCAGTGTTTTGCCTGAGGTGAAGAAGTGGGGGGCACTTTCTTACTTTCATTGTGTT ATTTTGGGTGACCCCCCTGCTCACTCTCGTGACCTCCACTTCCAGACAACATGGCAGCCGGTGTTGCAGC ATGGCTGCCCTTTGCCAGGGCAGCCGCCATTGGGTGGATGCCTGTTGCTTCGGGGCCTATGCCTGCGCCC CCAAGACAGGAGAGAAAAAGGACTCAGGACGCTCTGATAGTGCTGAACGTGAGTGGCACCCGTTTCCAGA CATGGCAAGACACCCTGGAACGATACCCAGACACTCTGCTGGGTAGTTCTGAGAGAGACTTTTTCTACCA CCCAGAGACCCAACAATACTTCTTTGACCGTGACCCGGACATCTTCCGCCACATCCTCAACTTCTACCGC ACGGGGAAGCTTCACTATCCCCGCCATGAGTGCATCTCGGCTTATGATGAAGAACTGGCCTTCTTTGGCC TCATCCCAGAAATTATTGGCGACTGCTGTTATGAGGAGTACAAGGACCGCAGGCGGGAGAATGCTGAGCG CCTCCAGGATGATGCAGACACTGACAATACAGGAGAGAGTGCGCTGCCCACCATGACTGCTAGGCAGAGG GTCTGGCGGGCCTTTGAGAATCCCCACACCAGCACCATGGCCCTGGTGTTCTACTATGTGACTGGGTTCT TCATTGCCGTCTCAGTCATCGCGAATGTGGTGGAAACAGTTCCATGTGGGTCTAGCCCAGGCCACATAAA AGAACTGCCTTGTGGGGAGAGGTATGCGGTGGCCTTCTTCTGCTTGGATACCGCCTGTGTCATGATCTTC ACAGTTGAGTACTTGCTTCGCCTGGCCGCAGCACCTAGTCGTTACCGTTTCGTGCGCAGTGTCATGAGTA TCATCGACGTGGTGGCCATCCTACCCTATTACATTGGGCTGGTGATGACAGACAATGAGGATGTCAGTGG AGCCTTTGTCACACTCCGAGTCTTTCGAGTCTTCAGGATCTTTAAGTTTTCCCGCCACTCTCAAGGGCTG CGTATACTGGGGTACACCCTGAAGAGCTGTGCATCAGAACTGGGCTTCTTGCTCTTTTCTCTCACAATGG CTATCATCATTTTCGCTACCGTTATGTTCTACGCAGAGAAGGGCTCTTCAGCAAGCAAGTTCACCAGCAT CCCGGCAGCCTTCTGGTACACCATCGTCACCATGACAACACTGGGGTATGGCGACATGGTACCAAAAACC ATAGCAGGGAAGATTTTTGGGTCCATCTGCTCACTGAGCGGAGTCTTGGTCATTGCGCTACCCGTGCCTG TGATCGTGTCGAACTTCAGTCGGATCTACCACCAAAACCAACGAGCAGACAAACGAAGGGCACAGAAGAA AGCCAGGCTGGCCAGGATCCGAGCAGCCAAAAGCGGGAGTGCAAATGCCTACATGCAGAGCAAGCGGAAT GGGCTACTGAGCAACCAGCTGCAGTCCTCGGAGGATGAACCGGCCTTCATAAGCAAATCTGGATCCAGCT TTGAGACACAGCACCACCACCTGCTTCACTGCCTGGAGAAAACCACGAACCATGAGTTTGTCGATGAACA AGTCTTTGAAGAAAGCTGCATGGAAGTTGCCACCGTCAATCGCCCATCAAGTCACAGTCCCTCTCTCTCC TCACAACAAGGAGTCACCAGCACTTGCTGCTCACGGAGACACAAAAAGACTTTCCGCATTCCTAATGCCA ATGTGTCGGGAAGCCATAGAGGCAGTGTGCAAGAACTCAGTACAATTCAGATCAGATGTGTGGAGAGAAC TCCACTATCCAACAGCCGATCCAGCTTAAATGCCAAAATGGAAGAGTGTGTTAAACTAAACTGTGAACAA CCTTACGTGACCACAGCAATAATAAGCATCCCAACACCTCCAGTAACCACCCCAGAAGGCGACGACAGGC CCGAGTCTCCTGAGTATTCGGGAGGAAATATCGTCAGGGTGTCTGCCTTGTAAGACAATTGCCGCAGGTC TGAGAGAATTTGAGCCCTGACTATGGAAAGAATCTAAAGGAGAAGAAAGAAGAAATAATAACCCCTTTTT GCATATTTAGACAGCAAAGCAAGAAGGTTGGCGATGAAACAAAAAAATCAAGATTTCAGAGTTGAAGATG TGAATCAAACCACCAAAAGGCATTTCTAGACAGCGTTCAACCTGTCCACAGAGGGTGACTCTGTGGCCCT TTGACTTTGTGAATGAGCACAATGAAATGCCATTTATTGATGCCTCTTATGATCAGAACTCTTTTTTAAT AAAACAAATAAAACAAATCTTTGGACATAATGTTCCAGTTGAATGCAAAACAAAAAAAATATGGAAAACA TTTTGATAAAAATTTTTCCTGTTAAAAACCATGAACATTGGCTCTGATGAAGATTGCTATTGATGAAAAA TTCATACGATCCATTTGTGTGGACTGAAGGAAACCATCCCAATGCATGCTAGAATTCTTTGGAGCAGTGA TCTCAGTTTCCTTATGTTGTCTTCAGAATAGGCATGACAAACTATACCTGTAGAAAGGGGAAATTTCTGT GCACTTACAATGAGCTGATTGTCCATGTTCTATGGTGGGCTGTGCAAATAAACTCCTTTTAGCACCACAG TGTTTCTCATGGGGATGCACACTAGTATATCTAAAGTGTTCGGATAGTTCAGTATTAATTATTGTTTAAC CTTGCACCTAGTCATTGTTATAACTGCAATAATTCATTCTATATCTGTTGTATCGGGAACCAGGATTTTT TTTTTGGACTTTCCATATATTCATAGATACAGTGAGAGCCACATTCATAGAAAAACTCCTGGTGTGGCCG GGTTTTAGCTGACTTTTTAATCCAAAACTACTGTGTCATAAATGTATTTTGGTAGTATTCTTTGGTCCAC TGTATTCCTGTGACCCAGTGCATTATCTGTCCCTGGATCTCTTTAGTGTCTTTCCACTGAGCTTACCCAC TAATGACAGGACTAGTGTCTACTGTAGTAGTAAAACAAACGTTCCCTGCCTTTGTATTTCTCAACCAGTT ATTATAAGAAGACTGTCACCAATCCCCTTGATAACAACTAGAGTAAAGGAGTTGACATGAAATATCTCCA GTGTTTCACTATTTGTAGAAAACCCAGACCCTGCAAACACCCATATGTGTTTTATGAGAGGCGTTAAATG GTACTTTTAAAAAAATCATAGGATGGATGATTTCAGAATCTAACTCAATGTATTAATTATTTTGCTTCAG ATCTTCAATACATTTTGAGGACAACTGGAATGCAGAGGAAAACTGAAGTGCTTTGTATAGCAATATTTGA TGAGAGCATGCTTTAAATGCCATCCAAGAAAGCAACAATGTATCTCAAAAGATTCTAGTGTATCACAAGG CACAAAGTTCTCTGCAAAATCAGGAAGAGGGAGTATGGTGATGTGAAGTCGACCACTGTGTACATTTAAG TGAAAAGTCTTGCTACCTTTTCACCCTTGAATATCCGCAGATGTGTCTGCACGTGACAATGTAAAAATAA AAAAAAAGTTTTATGTCAAATGAAAAGTTTTGTTCATTCCAAGCCACCACTGTAAGAACGAAAGCTTAGT TTCTATACATGAAAAAGAATTAATAATTAGTCCTCTGACATAGAGATTTTGAAATGCTTTTCACAATTTA TCATAAGAAAATGAATTAATCCAAACATTTTCAGGTAAAGCCAGAAATGGTCACTTCCCATTTATAGAAT AGCTTCTAGGACAGTGCTAGGCACACAGTAGCTCTTCATCAAAGCTGAGTGACAGGGAAAAGAACTCAGC TGACCCTCGGGAAGACCTGGCTTTACTCCTAGTGTGTCTTTGAAACAGTTACTAATATTCCTGAGACGTG AATATTAACAATTCTATTAACACCTGCCTGTTGTCACTTTATGCTTCTAGAGCAAATGTATAGTGAAACT TCTTTGATGGCACTTATTAAAAACTCTTTTAAAGTAAGTACAATCAGGAACATTTACCATCTTTTGATTC CCAATAAGAAATTCTATTTTCATGTTCCTAATAGGACATAAAACAACATAGACTTGGCTTATTTGGGTTG ATACTTATTATTCCTGCTTTGGTTTGTCGTTTATCACAAATTATTAATTTCTTCAGATTTTCAGAAGTGA AATTTTAAAAATAATCTTTTGGATGTTTTAGGTGATTTAAAATATACTGTGTTGGTGGTGAATGACTTTT GATGACGGTGTTAAGTGCAACTGTACTGTAAGTGAAATGTAATTATTTCTGTGTATCATATGGAGTAACT AAGGTCATTGTTTTTGACAATTTTGTTTGAAATTCATATATCTTATTTCAAAGGATAGCATAATATCTGC ATTATGCTGGAAAAAAATAGACCTTTGGAGAATACTTAAATAAAACATGTGCATGCTTGAACAGGAAAAA AAAAAAAAAAAA
• Restriction Sites: AscI-NotI
• ACCN: BC079667
• Insert Size: 1893 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC079667, AAH79667
• RefSeq Size: 4562 bp
• RefSeq ORF: 1893 bp
• Locus ID: 16508
• Cytogenetics: 6 8.49 cM
• Gene Summary: Voltage-gated potassium channel that mediates transmembrane potassium transport in excitable membranes, primarily in the brain, but also in rodent heart. Mediates the major part of the dendritic A-type current I(SA) in brain neurons (PubMed:10818150, PubMed:17122039, PubMed:18045912, PubMed:18187474, PubMed:20371829, PubMed:22815518). This current is activated at membrane potentials that are below the threshold for action potentials. It regulates neuronal excitability, prolongs the latency before the first spike in a series of action potentials, regulates the frequency of repetitive action potential firing, shortens the duration of action potentials and regulates the back-propagation of action potentials from the neuronal cell body to the dendrites (PubMed:10818150, PubMed:17122039, PubMed:22815518). Contributes to the regulation of the circadian rhythm of action potential firing in suprachiasmatic nucleus neurons, which regulates the circadian rhythm of locomotor activity (PubMed:22815518). Functions downstream of the metabotropic glutamate receptor GRM5 and plays a role in neuronal excitability and in nociception mediated by activation of GRM5 (PubMed:18045912). Mediates the transient outward current I(to) in rodent heart left ventricle apex cells, but not in human heart, where this current is mediated by another family member (PubMed:9734479, PubMed:10601491, PubMed:11909823, PubMed:23713033). Forms tetrameric potassium-selective channels through which potassium ions pass in accordance with their electrochemical gradient. The channel alternates between opened and closed conformations in response to the voltage difference across the membrane (PubMed:9734479, PubMed:22311982). Can form functional homotetrameric channels and heterotetrameric channels that contain variable proportions of KCND2 and KCND3; channel properties depend on the type of pore-forming alpha subunits that are part of the channel (PubMed:11909823). In vivo, membranes probably contain a mixture of heteromeric potassium channel complexes (PubMed:11909823). Interaction with specific isoforms of the regulatory subunits KCNIP1, KCNIP2, KCNIP3 or KCNIP4 strongly increases expression at the cell surface and thereby increases channel activity; it modulates the kinetics of channel activation and inactivation, shifts the threshold for channel activation to more negative voltage values, shifts the threshold for inactivation to less negative voltages and accelerates recovery after inactivation (By similarity). Likewise, interaction with DPP6 or DPP10 promotes expression at the cell membrane and regulates both channel characteristics and activity (PubMed:22311982).[UniProtKB/Swiss-Prot Function]