Efnb2 (BC057009) Mouse Untagged Clone

CAT#: MC206077

Efnb2 (untagged) - Mouse ephrin B2 (cDNA clone MGC:67047 IMAGE:6827408), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Efnb2
• Synonyms: ELF-2, Htk-L, LERK-5, NLERK-1, Eplg5, Lerk5
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC057009
  CTGCGCTCCAGGACATGGAACCGCGCCGACGCGGCGCTCGCTTGCCCGGGCCACTGCCCGCTGTCCTGGA TCTATAGTCAGGCGGCCCCTCTCGGGGAGCAGCACCCGCCGCCTCACACCGACTCGTCTCTCCGCTGCGG GCCTTTGTCCCGGCCCAGTGCCCTCGCCGCGCCCGCCCTCCGCGGCTGGCCCGGCACCCTGGAGCCGCAC GGGAGCCGACTGTCCCAGCTGCGTCCGGCGCAGCGCCCCGGAGCCCCGAGTCCGCCGAGCCGCCGCGCCC CGCGCGTTCGACCCCACCCCGCGCGCTGAGGGACGCGCAGGGTGAGCGCACCTGGCCTCGGCGACCGCGG GAGCGGCGCGGCGTGTCCGCCCCGGAGGATTGGGGGTCGCTGCCCGCGGCCGGTCCCAACGCGTCCCGGA GTGCGCAGAACTGGGAGCGGCTTGGGCATGGCCATGGCCCGGTCCAGGAGGGACTCTGTGTGGAAGTACT GTTGGGGACTTTTGATGGTTTTGTGCAGAACTGCGATCTCCAGATCGATAGTTTTAGAGCCTATCTACTG GAATTCCTCGAACTCCAAATTTCTACCCGGACAAGGCCTGGTACTATACCCACAGATAGGAGACAAATTG GATATTATTTGCCCCAAAGTGGACTCTAAAACTGTTGGCCAGTATGAATATTATAAAGTTTATATGGTTG ATAAAGACCAAGCAGACAGATGCACAATTAAGAAGGAGAATACCCCGCTGCTCAACTGTGCCAGACCAGA CCAAGATGTGAAATTCACCATCAAGTTTCAAGAATTCAGCCCTAACCTCTGGGGTCTAGAATTTCAGAAG AACAAAGATTACTACATTATATCTACATCAAATGGGTCTTTGGAGGGCCTGGATAACCAGGAGGGAGGGG TGTGCCAGACAAGAGCCATGAAGATCCTCATGAAAGTTGGACAAGATGCAAGTTCTGCTGGATCAGCCAG GAATCACGGTCCAACAAGACGTCCAGAGCTAGAAGCTGGTACAAATGGGAGAAGTTCAACAACAAGTCCC TTTGTGAAGCCAAATCCAGGTTCTAGCACCGATGGCAACAGCGCGGGGCATTCCGGGAACAATCTCCTGG GTTCCGAAGTGGCCTTATTCGCAGGGATCGCATCAGGATGCATCATCTTCATCGTCATCATCATCACTTT GGTGGTGCTGCTGCTCAAGTACCGCAGGAGACACCGCAAACACTCTCCACAGCACACGACCACGCTGTCT CTCAGCACACTGGCCACGCCCAAGCGAGGTGGCAACAACAATGGCTCGGAGCCCAGTGACGTTATCATAC CACTAAGGACTGCAGACAGCGTCTTCTGCCCGCACTACGAGAAGGTCAGCGGGGACTATGGGCACCCGGT GTACATCGTGCAGGAGATGCCCCCACAGAGTCCTGCCAACATTTACTACAAGGTCTGAGGCCTGAGACCT GCGCCTCCCAAGGGAACTCGCACCTTGTTCTTGGGCACGCAGGGACTGCCTGAGCCTGGCTGTGGGGGCA GGATGCCTCCTGGAAGAGCCTGGATCTGGACAGTTTTGTAGTCTGTAGCTTTTCCGACCCTGGGGACCAC AGACCCTCCCCGGAAGCTGGAAGACTGCTAGGAGATCCCCACTTGGACTGCCGCGGCCCCACGCGGACCT CCAAGCCATGCACCCAGCCACTCAGGCCTCTGCAGAGCCCGGGGAGGACACGGTAGGCTATGGATGGCGC AGCAGCATCTTAGGAGAAGGTTGCGCACCAGGCCGGCCCCTGCCTCCACGTTTCCTGCCGTGCACACTGG ACTTATCACTTGGACCTCGGGTTCAGTAAGGTTTTCAAAGATCTCTAGTGTTTAGTCCTCACTCACTCAC TCACTCACTCACTCCTTCTCTTGCCAGGGCTCTGCAGCAAACTCCCTAGACCCCTCACTCCACGTACTGC ATCATTACGGGACACTCACCACAGAGTCCCAGCTCCACCCTTTACACCAAGATCAAATTAGATGGGTATT AGGTACAGAAGAACCCTGCTTGCCTGGAGGCCGGGTCAGCCGGGAAGCGCAGATGTGTGGAGGAGTGAGG AGGTGCTGGCTGCCACGGCAGGTCAAGGCTGCTTGCTGCCCCTGGAGCATAGTAGGGATGCAGGAAGGAA ATAGATAATGCTTTGGTTTTTCTGAGAGGACAGAGACAGGTGGGAGGTGACTGACTGGTGAGTGGTGGGG AGCCTTTCACTACCACACAGCTATGCAGCAGGGAATCAAAAGTCCCTCTCCTGCGGGGAACAAAGGGGCC ATTGTTGTGAAAGGACCAGCTAGAGCACAGAGGGAGAGGGCAGGCCTCCGGTGAAGTGCTGGGGCAGAAC TGCAGAGGTACTGGAAATAAAAAGCGCAGCGCAGAGCTGTGGGAGAGTCCGTCTGCTTTGGGAGATGTTT TAAGCAGACTCAGCTGCTATATTACCACGTTTTTATTAAAAACACAGGGAAAGCATTTAGGAGAAGAGCA GAGAGCCAAATCTGACCTAGAAGTTGAAAAGCCAAAGGTCAAACAGGCTGTAAGTCCATCACCACTGAGG TTATTGGAGAATTCTCATTAGGAAAGGCAGGTCAGATTCCCCAGGCCCCATAAGTGCCCCTTCCCCCTCC CTGATTGAGCCTTACACGTTGGTTTTTGGTTTATGGCCGTGCTGTCCGGGCTCCAAGGCAGTACCCGGGC TCCATGTCAAAGCAAAGCACACATGGCCCACCTCTTAGAGTCCTTGAGATGGAAGTAAGTTATGCCGCGG AAGGAAAGGCGAAGATAGGACATATTTATAATAGGTGTATAGAACACAAGGGATATAAAATGAAAGATTT TTACTAATATATATTTTAAGATTACACACAATACACACCAGAAGACGTGGAGTTCGGTGGTGGTGGTGGT CGTGGTGGTGGTGATTAAAGTGACCCCAGCGCTTAGTGCTTTAAAAAGTGAAAGATTGGGTAGCTACTCC CCGAAACGTACCAATAGCAAGAAAAGTATCCATAATGAGAGCAAATGGCAAAAATAACACGGTCCTGCGG GAATCTCGCAGAACCGTAGACTAGGAATGCCAGCCCCCCAAATTGATGTGACCCTGCCCCGGGTTAGACA ATGATAAAATGCGCTGGCCTTTATTTTCTGTGTTGGGTTTTCCCTTGCCTTATGGGCTGAAGTGTTCTCT AGAATTTAGCAGGTCACACTGAGGGGATTCCAGTTTAACTGTGGGTCCCTCCTCCTCTCCTACCCCATCC CTGCCCTTCCAGAGAATAACAGGAAGCCTTCCTTTTTTTTTTTTTTTAAGTGCTATGCAAAAGAGACATC TTTAACAGAGTCCTGTTACTATGGTAACATTTTGCTTTCTGAATTGGGAGGAAATAAAAATTGTAATGAC AGCATTTGAAGGTTCTCAGACCTCCAGTGAGTACCTGCAAAAATGAGTTGTCACAGAGATTATTCCCTAC TTCTCAAACCTGAAAATGATGTTGGTTCGATGTGTGTGTGTGTGTATGAGTGGGTGTGTGGTACATGTGT GTACATATATGTATAATATATATCTCCAGTATATATTATATATATCTATATCATATTTCTGTGGAGGGTT GCCATGGCAATCAACTGCAGTACATATGTAGTTCTTTCCATCACCCTAACCTCTCCTGCGCATTCACACA AGAGTTTCTTGTAAGCCATCAAAAGTTAATTCTAGGGGGAGAGGGATGAGGCGGGGAGACATGGGAAACC GTCTGATTTTAATGAAATCAAATGTCTGTGTCATCGGTTGGCTACGTTTTGGTTCTATGCTAAACTGTGA AGAATCGGATGAATTGATGAAGAGTTGAGTTACCTGCAACCCATTGAGAAGTGTCCTGTGCGTCTGTTTT GTGTCTGGTGCAGAAAATGACAATCTACCAACTGTCCCCTTATTTGGAGTTGGTTCAGCTTTGGAAAGTT ACTGTAAATGCCTTGCTTGTATTATCATCCCTAGTCACCTGACTTCGGAGCTTGCACCATCGTGTTTTAA GTGAAGACGCTGTAAATAGGTTCAGATCTTACCGTCTATGGATTCGGGTGTTACAGTAGCCTTATTCACC TTTTTAATAAAAATACACATGAAAACGAAAAAAAAAAAAAAAAAAA
• Restriction Sites: AscI-NotI
• ACCN: BC057009
• Insert Size: 1011 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC057009, AAH57009
• RefSeq Size: 4176 bp
• RefSeq ORF: 1011 bp
• Locus ID: 13642
• Cytogenetics: 8 3.42 cM
• Gene Summary: Cell surface transmembrane ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Binds to receptor tyrosine kinase including EPHA4, EPHA3 and EPHB4. Together with EPHB4 plays a central role in heart morphogenesis and angiogenesis through regulation of cell adhesion and cell migration. EPHB4-mediated forward signaling controls cellular repulsion and segregation from EFNB2-expressing cells. May play a role in constraining the orientation of longitudinally projecting axons.[UniProtKB/Swiss-Prot Function]