Evc2 (NM_145920) Mouse Untagged Clone

CAT#: MC205922

Evc2 (untagged) - Mouse Ellis van Creveld syndrome 2 homolog (human) (Evc2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Evc2
• Synonyms: 1110017L09Rik; Lbn; limbin
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC064473
  CGGACGCGTGGGCGGAAGCTCCGCGACTCTGCGGGGCCTGTGTGGGGTCTGTAGCCACCAGATGGGGGCG ACGGGCCCCACGGGCGCTGGGGGACGAGCCACGTGGGTGCTGGCTGGGAATATCCTGGCCGCTGCCCTGG TACTGGGCTCGGGTCCGCGTGCACTTCCACCATCCTTCCCTGCCCTGGGCCCAGGGTCACCATCTCGGCC CGGCCCAGCAGGACCCTGGGCAAGCTCGCAGTACAGCGATATTTCAAGAGAAGCCCGAGGTCCCTTTGAG AATGGAGTGATATTTCAAAAATGCTCACTGGTGTCTGGGCAGAGCGAATCGCAGACCATGCACGTGCAGC TGTCCGTGAACAACACCAGGACGCCCACATCAGTCAACCTTTCGAACCTGCTCGTGCTAGATGAGATCAC TGGCCTTGCTGTGAAGGAGTCGCCTGGAAATAATACTCAGGATGGAATCCAGACTTTCAGAAAGAGCTTT CTGCAAGTGGGAGAATGCTACTCTGTCAGCTACACAGCCTCGCTGGACCCCACAGCCCTTGGGACTGGGG AGAGCCTGGATCTTCCTGCCCGGCTCATCTTTCAGAGCCCCTCACAGAACAGAACTCAGCTAAAAGCCCC CTTTACCATCACAGTGGAAGAAAAGATAATGGTTCTTCCTAACCATGGTCTTCACGCAGCAGGCTTCATC GCAGCCTTCCTCATCTCCCTCCTCCTGACCGTGGCAGCCCTGTTCTTCTTGGCGAGGGGTCGATGTCTGC AGGGAGGCATGCTGTCCAGATGCCGGATTCAGCATCCTGAGAACAAGCTGGAGCCCTCGCCCTTCACCTC TGCTAACGGTGTGAGCCAGGACCTTTCCCTCAATGACCAAGTGGTTGCCATCCTGACTTCTGAGGAGCCT GGGAGCATGCTTCAAGCCCTAGAAGAGTTGGAGATCGCAACCCTGAATCAGGCAGATGCGGATCTGGAGG CTTGTCGAAACCAGATTAGTAAGGACATCATTGCCCTTTTGATGAAGAATCTGGTCAGCGGTGGTCACCT CTCTCCGCAAACAGAAAGGAAGATGGCCGCTGCTTTCAAAAAGCAGTTTCTGCTGTTGGAAAATGAAATA CAGGAGGAGTATGAGCGGAAGATGTTGGCACTGACGGCAGAGTGTGACCTAGAGATGAGGAAGAAGACAG AAAACCAGTACCAGAGGGAGATGGTGGCCATGGAGGAAGCAGAAGAGGTGTTGAAGAGAGTTAGCGAGAG GTCTGCTGCCGAGTGCAGCAGCCTTTTGCGGACCCTCCATGGGCTGGAGCAGGAGGACATGCAGAGGTCC CTTACTCTGGACCAAGCCGAGGACTTTGCTCAAGCACACAGGCAGCTGGCTGTTTTCCAGAGGAATGAAT TACACAGCATCGTCTATACCCAGATACAGAGTGCTGTCTCCAAAGGGGAACTGAGGCCTGAGGTGGCTAA AATGATGCTGCAAGACTACTCTAAAACACAGGAGAGTGTAGAAGAGCTGATGGACTTCTTCCAGGCCACC AAGCGATACCATCTCAGCAAGAGGTTTGGCCACAGAGAGTACCTGGTCCAGAGACTGCAGGCCATGGAGA CCCGGGTGCAAGGGCTGCTGAACACCGCGGCTACCCAGCTAACAAGCCTCATCCATAAACATGAGAGGGC TGGCTACTTGGATGAAGATCAGATGGAAACGCTATTGGAGCGTGCACAGACAGAAACCTTTTCTATAAAA CAGAAGCTGGACAATGACTTAAAGCAGGAAAAGAAAAGGCTCCACCAGAGATTAATAACCAGGAGGCGGC GAGAGCTTCTGCAGAAGCACAAGGAGCAGCAGAAGGAGCAGGTGTCCCTTGGCGAGGCCTCCAGCACCGC GGAGGACGCAGTCCAGTACCTGCACCAGTGGAGGAGCGTGATGGCCGAGCACACCGCTGCCCTGGAGGAG CTGCAGGAACGTCTGGACCAGGCTGCCCTGGATGACCTCCGGGTCCTGACTGTGTCGCTGTCGGAGAAAG CCACGGAAGAGCTGAGGCGCCTGCAGAGCACGGCCATGACACAGGAGCTGCTGAAGCGCAGTGCTCCCTG GCTCTTCCTGCAGCAGATCTTAGAGGAGCACAGCCGGGAGTCTGCGGCTCGCACCACGCAACTGGAGGCA GAGGAGAGGGAACGTGGCCAGGAGCTGGTGCAGGGCGTGAGGCAGAGGCTACAGCAGGATGCACTGGAGG CCTACACGGAAGAGCAGGCAGAGCTGAGGCACTGGGAGCACTTGGTGTTCATGAAACTCTGCTGTGCGGC CATCTCATTGTCTGAAGAAGACCTGCTCCGGGTAAGGCAGGAGGCACAGGGCTGCTTCAGCCAGCTGGAC CGGAGCTTGGCCCTCCCCAGAGTCCGTGCACGAGTGTTGCAGCAGCAGGCGCAGATGGCCTGGCGGGAAG CAGAGTTTCGGAAACTGGACCAGGCCCTGGCTGCGCCTGAGCTGCAGTCCAAGGCAAGAAAGCTGCGTTC CAAGGGCAGAGGCAAGGCCGATCTTCTGAAGAAGAACCTGGAGGATAAGATCCGACTATTCGAGGAGCGG GCCCCTGTAGAACTGGCTGACCAGGTGCGAGGGGAATTGCTCCAAGAGAGGGTCCAGAGGCTGGAGGCCC AGGAGGCGCACTTTGCTGAGTCACTTGTGGCTCTGCAGTTCCAGAAGGTGGCCCGGGCTGCTGAGACCCT CTCAGTCTACACCGCCCTGCTCAGCATTCAAGACCTGCTGCTGGGGGAGCTGAGTGAGTCGGAGACCCTG ACCAAGTCAGCCTGTGTGCAGATCCTGGAGTCCCACAGGCCGGAGCTGCAGGAGCTGCAGGAGCTGGAGA GGAAGCTGGAGGACCAGCTGGTGCAGCAGGAAGAGGCCGAGCAGCAGCGGGTCCTGGAGAGCTGGCAGCG GTGGGCGGCCGATGGACCTGGGCTGAGCGAACCTGAGGAGATGGATCCTGAAAGGCAGGTCTCTGCCATC CTGCGGCAAGCCCTGAACAAGGGCCAGAAGCTGCTGGAGCAGCATCAGCAGAGGGTGAGAGAGGAGTGGC AGAACGGTGCGGTGCTGGAAGATTCTCTGGAAAGCATTGAAGCTGACACCATGGCCAGCCTTTGCAGCCA GGGACTGAGGCTGGTGTCATACCTCTCAAGGATGACAATGGTACCAGGCAGCACACTACTGCGACTCCTG AGTGTGGTGCTTCCAGCGGCTTCACAGCCTCAGCTACTGGCCCTGCTGGACGCAGTCAGTGAGAAGCACT CCGACCACACAGCTGAGAATGAGAGCAGTGGTGAGCAGGCTCAGGCAGAGCAGAGCAAGAGGAGGAAACA CCAAGTTTGGTGGAAGGTTTTGGACAGCAGATTCAGAGCAGATCTGGTGAGTCAAGGACTGGAGAGGATG CTCTGGGCCCGCCAGAAGAAGGAGCGCATATTGAAGAAGATATATGTTCCCGTCCAGGAGAGGGTGATGT TTCCTGGGAAAGGAAGCTGGCCACACCTGTCCCTAGAACCCATTGGAGAACTGGCTCCCATCCCCATCAC TGGGGCAGACGCTATGGACATATTAAACACGGGAGAAAAGATCTTTGTATTCAGAAGCCCAAGGGAGCCC GAGATCTCTTTGCGTGTTCCTCCCAGGAGGAAAAAGAATTTCCTGAATGCCAAGAAGGCCAACAGGGCCT TGGGCTTGGACTGACCCAGGGGGAAGCACCAGGGGCTGTCTCAAGACAGGTGCATCTTTCCTCCAGCCCA CGTGGGTTGGTCTGTCCGTCTATATCATACAGCTTGCAGTATACATATGCTTGCAGCCTTGACACAGTGC CACGCAAGTTGGCCCTCCGGTGTTCAGGTCCAGTCTCTTTTGTGCCACAGGACACAAACATACCTTCCTT CTAAGTCCGTATTACCATGATCTAGAGGGAACCCACCTTACCCCACCTTCCTCACACAGACCACTGGCTG TGGCTGGGATGGGTCCCCACCCCACCCAGTTTATGTGAGGAAGGTGCAGCTCTCTATTCTTACAATGTGT GTTACATGCTCTGTTTTTCCATAAAGGGAACTTTAAAAATGTAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_145920
• Insert Size: 3663 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC064473, AAH64473
• RefSeq Size: 4117 bp
• RefSeq ORF: 3663 bp
• Locus ID: 68525
• UniProt ID: Q8K1G2
• Cytogenetics: 5 B3
• Gene Summary: Component of the EvC complex that positively regulates ciliary Hedgehog (Hh) signaling (PubMed:21356043, PubMed:24582806). Plays a critical role in bone formation and skeletal development (PubMed:21356043). May be involved in early embryonic morphogenesis (PubMed:21356043).[UniProtKB/Swiss-Prot Function]