Ncf1 (NM_010876) Mouse Untagged Clone

CAT#: MC205906

Ncf1 (untagged) - Mouse neutrophil cytosolic factor 1 (Ncf1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ncf1
• Synonyms: Ncf-1; NCF-47K; NOXO2; p47-phox; p47; p47phox
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC055836
  CACGGCCCAGCCATGGGGGACACCTTCATTCGCCATATCGCCCTGCTGGGCTTCGAGAAGCGCTTCATCC CCAGCCAGCACTATGTGTACATGTTCCTGGTTAAGTGGCAGGACCTGTCGGAGAAGGTGGTCTACAGAAA ATTCACCGAGATCTACGAGTTCCATAAAATGCTGAAGGAGATGTTCCCCATTGAGGCCGGCGAGATCCAC ACAGAGAACAGAGTCATCCCACACCTCCCGGCACCCAGGTGGTTTGATGGACAACGAGCCGCTGAGAGCC GCCAGGGCACGCTCACTGAGTACTTCAACGGCCTCATGGGACTGCCCGTGAAGATCTCCCGCTGCCCACA CCTGCTGGACTTCTTCAAAGTGCGGCCTGATGACCTGAAACTGCCCACTGACAGCCAGGCCAAGAAGCCA GAGACGTACCTGGTGCCCAAAGATGGCAAGAATAACGTAGCTGACATCACAGGCCCCATCATCCTTCAGA CCTATCGGGCCATTGCTGACTACGAGAAGAGTTCGGGAACAGAGATGACCGTGGCAACCGGAGACGTGGT GGACGTCGTGGAGAAGAGCGAGAGCGGCTGGTGGTTTTGCCAGATGAAGACAAAGCGAGGTTGGGTCCCT GCATCCTATCTGGAGCCCCTTGACAGTCCCGACGAGGCGGAGGATCCGGATCCCAACTACGCAGGTGAAC CGTATGTAACCATCAAAGCGTACGCTGCTGTTGAAGAGGACGAGATGTCCCTGTCTGAGGGTGAAGCCAT TGAGGTCATTCATAAGCTCCTGGATGGCTGGTGGGTGGTCAGGAAAGGGGATATCACCGGCTATTTCCCA TCCATGTATCTGCAGAAGGCTGGGGAGGAGATAACCCAGGCCCAGCGACAGATCAGAGGCCGCGGGGCAC CACCTCGAAGGTCGACCATCCGCAACGCACAGAGCATCCACCAGCGTTCTCGGAAGCGTCTCAGCCAGGA CACCTATCGCCGCAACAGCGTCCGATTCCTGCAGCAGCGCAGACGCCCGGGGCGACCCGGGCCGCAGAGC ACGGATGGCACAAAGGACAATCCATCGACTCCGCGCGTCAAACCACAGCCCGCGGTGCCTCCGCGACCCA GCTCAGACCTCATCCTGCACCGCTGCACAGAGAGCACCAAACGGAAGCTGACGTCCGCTGTGTGAGAAGC AGCGGCCCTAGACGGCTCCTATCCCTATCTCTGTATATACTTGTGTATAGCCTCAGGTTAGAGACTCCTC CCTTGCCTTAAGGTTTGGAATGGACTTAGACTCTACAGCAAAGGACAGGACTGGGTTAATCTCCACCGGT ATTGCTAGCATGAGAGGAGGCAGTGAGCGCTGCTAGGGTGGGGGTTGCAGGGACAAAAAAGATCTGATTA CCTGCTGCTGTAGTCTCGACCAAGTTTGCAGAAAGCCAAGTGCCTCAGGAACGCCCTAGGAAAGTGTTTC TCAACCTGTTGGCCGTGACCCCTTTGGGGAGGGTGTCAAAAGACTTTCCCAGAGGTTGCCTAAGACCATT GGAAAATACAGATATTTATTTCTATAGATGTTTATATTATGATTCATAAAAGTAGCGAGATGACAGTTAT GAAGTAGCAATGAAAATAATTTTATGACTGGGGGTCCCCACAACATCGGGAACTAGCTGTATTAGGGTCA CAGCACTAGGGAGGTTGAGAAGCACTGCCCTAGGGCCTCAGGATGTTGCCCGGTTGGTAGAGCTTGACTA GCATGCAACCGGGCCTGGCTTCCATTCCCAACATCCCATAATAGGGTGTGCCAGTGTCCCAGCATTAGGG CAGTCGAGCCGAGGCTCACATCTGTAATTTCAGAGCCAAGAGTCAAGAGAACTGCAGTTAGACTCAAGAT CGGCCTGGGCCATGTAGGGAATTTGAGATCAGATTTAGCTAGTGTGAGATCCTGGGTCATACCACCCCAT ACCACAGTATAACGGGCGTAGGGAGTCTCAGGGGGATGAAAGCGACTGTCATTAGTGGGCAGTTATAGAT TAATATTAGCAACTAATGACTAGTGTCATTTTAGTGTCATTAGTGATGCGGAGACTTTGCTTTCATGTCT CTATTTCCATCCCCAAATGCAAAGAAACCGCGCTGGCTGGTCTATGTCAACTTGACACGAGCTGGTGTCA TCTAAGAGAGGGAACCTTGATTGAGGAAACACCTCAGTGGTTAAGAGCACCAGCTGCTTTTCTGAGGACT GGTGTTCGATTTCCAGCATCCACATTTGCCTGTAACTCCGGTTTTAGGGCATCTGACACCCTCACACAGG CATATATGCAGACAGAAGACCAATGCACATAAATTAAAATACACACTGAAAAAAATTTAAAAAGAGAGAA TATGCCTCCATAAGATCTTGGTGATTAATGGGGGAGGGTCCAGCCCACAGTTGGTGGTGCCATCCTGGGC TGGTGGTGCCATCCTGGGCTGGTGGCCCTGGGTTCTATAAGAAGGCAGGCTGAGCAAGCGATGGGGAGCA AGGCAGTAAGCAGCCCCCTCCATGGCCTCTGCATCAGCTCCTGCCTCCAGGATCCTGCCCTGCTTGAGTC CTTGCCCTGCTTGAGTTCCTGTCCTGACTTTTTTTCAGTGATGAACAACATGGAAGTGTAAGCTGAATAA ATCTTTGCCTCCCCAAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_010876
• Insert Size: 1173 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC055836, AAH55836
• RefSeq Size: 2692 bp
• RefSeq ORF: 1173 bp
• Locus ID: 17969
• UniProt ID: Q09014
• Cytogenetics: 5 74.47 cM
• Gene Summary: NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1, resulting in an isoform (b) that is shorter than isoform a.