Ears2 (NM_026140) Mouse Untagged Clone

CAT#: MC205790

Ears2 (untagged) - Mouse glutamyl-tRNA synthetase 2 (mitochondrial)(putative) (Ears2), nuclear gene encoding mitochondrial protein, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ears2
• Synonyms: 3230401I01Rik; mKIAA1970
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC025907
  CGTGTGTGTTTCTAGTCCACATGGCGGCGCCCCTCAAGAGGCTTCTGCTGGCCGAGCCGCACGTGGTGGC CCTCGGGCACCGCGTGGGGCGGCGCGAGGCCAGTCTGGGCCCAGATCCCGGGGCCCCGGTGCGAGTGCGC TTCGCTCCCAGCCCCACAGGCTTCCTGCACCTGGGTGGCCTCCGTACTGCCCTCTACAACTACATCTTTG CCAAGAAGCACCAAGGGAGCTTCATTCTGAGGCTGGAGGACACCGATCAGAGCCGCTTGGTGCCAGGGGC AGCAGAAAGCATTGAGGACATGCTGGAGTGGGCAGGCATCCCACCTGATGAGAGTCCCCGCCGGGGTGGT CCTGCAGGGCCCTACTGTCAGTCCCAGCGGCTGGCTCTGTATGCCCAAGCCACGGAAGCCTTGCTGAGAT CTGGAGCTGCCTATCCCTGTTTCTGTTTGCCACAGCGGCTGGAGCTTCTGAAGAAGGAAGCACTGAGGAG CCGACAGACACCCCGGTATGACAATCGGTGCCGGAACCTGAGCCAGGCACAGGTGGCCCAGAAGCTGGCC GTAGACCCCAAGCCTGCTATCCGCTTCCGTCTAGAGGAGGCAGTACCAGCCTTCCAGGACCTGGTATATG GCTGGACTCAGCATGAAGTGGCCAGTGTGGAGGGGGACCCAGTTATCCTGAAAAGTGACGGCTTTCCCAC CTACCACCTAGCCTGTGTGGTAGATGACCACCACATGAGCATCAGCCATGTGCTGCGGGGCTCCGAGTGG CTAGTCTCCACCTCCAAGCACCTGCTCCTCTACCAGGCCCTGGGCTGGCAGCCACCTCGATTTGCTCATC TGCCCCTGCTCCTCAACAGGGATGGTAGCAAGCTGTCTAAGAGGCAAGGGGACATTTTCCTGGAACATTT TGCTGCCACCGGTTTTCTGCCAGAAGCCTTGCTTGACATCATCACCAACTGTGGCTCGGGGTTTGCAGAG AACCAAATGGGCAGGACCCTGCCGGAGCTGATTACACAGTTTGACCTGACGCGGATCACCTGCCACTCAG CCCTGCTGGACCTAGAGAAGCTCCCAGAATTTAACAGGCTGCACCTCCGCCGGCTGGTGAGCAGTGAGAC CCAGAGGCCCCAGTTGGTGGAGAAATTGCAGGGCCTGGTGAAGGAGGCATTCGGGAGTGAACTGCAAAAC AAGGATGTTCTGGACCCTGCATATATGGAGAGGATCCTCCTGCTGAGACAGGGTCACATCAGCCGCCTGC AGGATCTGGTCTCCCCAGTGTACTCCTACTTATGGACCCGCCCTGCTGTCCATCGATCAGAACTGGGTGC CAGCTCGGAAAACGTGGATGTGATTGCCAAGCGCTTGCTGGGGCTTTTAGAAAGACCTGGCTTAAGCCTA ACTCAGGATGTGCTGAACAGAGAGCTGAAGAAGCTGTCAGAAGGTCTGGAAGGCGCCAAGCATAGCAGTG TGATGAAGCTCCTCCGGATGGCCCTCAGTGGACAGCTGCAAGGACCCCCTGTGGCTGAGATGATGGTGTC CTTGGGCCCAAAGGAAGTACGGGAACGAATCCAGAAGGTGCTTTCTAGCTAGGGAGAAGATGCATAGCAC CATCGAGATGACCATGAGAGCCTCTAACCATGGAAACTGAATTCTGAGGAGAGCAGGAAACCTGGAGCCT ATTGGGACCCTTTCAGGATGAACAGCTGTAGAAACAAGCACTCTGACTACTCTCATCAGCTGGCCTTCGG GGACCCCAGCTGTTTCACTGTTGACTCTGGGAATAATGAGCTTGAACTTGTGTCTTGATTCTTTCCTATT TTTTTTAAAGACTTTTTAAAAATGTGAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_026140
• Insert Size: 1572 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC025907, AAH25907
• RefSeq Size: 1862 bp
• RefSeq ORF: 1572 bp
• Locus ID: 67417
• UniProt ID: Q9CXJ1
• Cytogenetics: 7 F2
• Gene Summary: This gene encodes a putative member of the class I family of aminoacyl-tRNA synthetases. These enzymes play a critical role in protein biosynthesis by charging tRNAs with their cognate amino acids. This protein is encoded by the nuclear genome but is likely to be imported to the mitochondrion where it is thought to catalyze the ligation of glutamate to tRNA molecules. Mutations in a similar gene in human have been associated with combined oxidative phosphorylation deficiency 12 (COXPD12). [provided by RefSeq, Mar 2015]