Sh3bp4 (NM_133816) Mouse Untagged Clone

CAT#: MC205718

Sh3bp4 (untagged) - Mouse SH3-domain binding protein 4 (Sh3bp4), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Sh3bp4
• Synonyms: AI594717; AW227605; BOG25
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC012284
  GCGGCGTCTGGGCACCTCACCACCTCTGCTGCCTCGATCGCTGAGGCCGCGGTACCGGGAGTCGCGCGCG CCCCCGGCCGGGTGAGCCCCACAGGACAAGAGAACCGCAGGACCCGTGGGGCGCCATGCGAGCCAGGGCA TCCCTCTTCTCGAGGATGGAAGGTGGAGTCTTGGAGTTTTTCCTCTGACATCAAGAGGCCAACATCCCCC AGCAGGAAGAAGCACACTGCCAAGTTGACCCTGCCGTGCAGCGTGACTTTATGAGACAGCAGCTGCTGTC CCTGCTGGAACTAGGGGAGGAGACATGAGACCATAGTACCTCACCCACCCAAGAGGCTCACCATGGCAGC CCAGCGTATCCGGGCAGCCAACGCCAGTGGCCTCCCTCGGTGCAAGTCGGAGGGGACTCTGATAGACCTG AGCGAGGGCTTCTCAGAGACAAGCTTTAATGATGTCAAAGTGCCTTCCCCCAGTGCCTTGCTCGTAGACA ACCCCACGCCTTTTGGAAACGCAAAGGAAGTGATTGCCATCAAAGACTATTGCCCAAACAACTTCACCAC CCTGAAGTTTTCCAAGGGCGACCACCTCTACGTCTTGGACACGTCTGGTGGGGAGTGGTGGTACGCTCAC AACACCACCGAGATGGGCTACATCCCTTCCTCCTACGTGCAGCCATTAAACTACCGGAATTCTACCCTGA GTGACAGTGGCATGATCGACAATCTTCCGGACAGCCCTGAAGAAGTTGCCAAGGAGTTAGATCTGCTAGG GGGTGGCTGGACCGATGACCAGAAAGAATCTGGCAGGCCCTATAGTAACAACCCTTTCTGGAATGGAGTC CGAACGAACCCCTTTCTGAATGGGAATGCACAGCCCAGTACGGATGAGCTAAACCCCAAGAGTACCGTGG ATTTGCTCCTGTTTGACACGGGCACATCTTCCTTCACTGAGTCCAGCTCGGCGACCACCAACAGCACCGG CAACATCTTTGATGAGCTCCCAGCCACCAATGGACTCCAGGTGGAGCAGCCTGTCAAGCGGGACAACCCC TTTTTCAGAAGCAAGCGTTCCTACAGCCTTTCGGAACTCTCCGTCCTGCAAGCCAAATCGGATGCTCCCC CAACGTCCAGCTTCTTCACAGGCTTGAAGTCGCCTGTCCCGGAACAGTTTCAAAGCCGAGAAGACTTCCG AACCGCCTGGCTCAATCACCGGAAGCTGGCCCGTTCCTGCCATGACTTGGATCTGCTCGGCCAAAGCCCA GGCTGGGGCCAGACCCAAGCAGTGGAGACAAATATTGTGTGCAAGCTGGATAGCTCTGGGGGCTCCGTGC AGCTTCCTGACACCAACATCAGTATCCATGTGCCAGAGGGCCATGTAGCTCCTGGGGAGACACAGCAGAT TTCCATGAAAGCCCTGCTGGACCCTCCGCTGGACCTTAACAGTGACAGATCCACCAGCGTAAGCCCTGTG GTGGAGGTGAAGCTGAGTAACCTAGAGGTGAGTACCTTCATCATCTTGGAGATGAAGGTCTCCGCCGAGG TGAAGGGCGACATCTTTAGCAAAAGCACGGTGGTCCTGCAGTGCCTGAGAAGTGACTCAAAGGAGGGGCC TTATGTGCCCATCCCTCTCGCCTACAGCTATGGGGACACCATACAGGTACAGCTGGACAACCTGGAACCC TGCATGTACCTAGCCATCGTTGCCCAGGGCCCCAACATCCTCTACCCTTCCACCGTGTGGGATTTCATCA ATAAGAGGGTCACCGTGGGTCTCTATGGTCCCAAACACATCCATCCATCCTTCAAGACAGTAGTGACCAT TTTTGGACATGATTGTGCCCCCAAGACCCTCCTGGTGAGTGAGGTGACTCGGCAGGCTCCCAGTCCTGCC CCCGTGGCCCTCCAGCTGTGGGGCAAACATCAGTTCATTCTGTCCAGGCCCCAGGATCTCAGAGTGTGTA TGTTCTCCAATATGACTAACTATGAGGTCAAGGCCAACGAGCAAGCCCGAGTAGTGAGAGGGTTTCAGAT GAAGCTGGGCAAGGTGAGCCGTCTGATCTTCTCCGTCATCTCCCAGAACCCCAATGAGCTGTCCGACTTC ACCCTGAGGGTCCAGGTCAAGGATGATCAGGACACGATCCTCACGCAGTTTTGTGTCCAGACTCCGCAGC CGCCCCCTAAAAGCGCCATTAAACCGTCGGGACAGAGGAGGTTTCTTAAGAAGAACGAGGTCGGGAAGAT CATCTTGTCCCCGTTTGTCGTTACAACCAAGTATCCAACGTTTCAGGACCGCCCCGTGTCTAGCCTCAAG TTTGGCAAGCTTCTAAAGACAGTGGTGCGGCAGAACAAGAGTCACTACCTGCTGGAGTACAAGAAAGGTG ACGTCGTGGCTCTGCTCAGCGAGGAGCGCATCCGGCTCAAGGGACAGCTGTGGACCAAGGAGTGGTACAT CGGCTACTACCAGGGCAAGGTGGGCCTGGTGCACACCAAGAACGTGCTGGTCGTCGGCAAGGCCCGGCCC AGCCTGTTCTCGGGGCCCGAGCTGAGCACCTCAGTGCTGCTGGAGCAGATCCTGCGGCCCTGCAAGTTCC TTACCTACATCTATGCCTCTGTGCGGACCTTACTCATGGAGAACATCAGCAGCTGGCGGGCCTTTGCTGA TGCCCTGGGTTACGGCAACCTGCCGCTCACCTTCTTCTGCCGGGCAGAGCTGGACAGCGAGCCGGAACGG GTGGCTTCCGTTCTGGAAAAGCTCAAGGAAGACTGTAACAACCCTGACAACAAGGACCGGAAGTCCTTCC AGAAGGAGCTGGTGATGGCTTTGCTGAAAATGGACTGCCAGGGCCTGGTGGTGAGACTCATCCAGGACTT TGTGCTCCTGACCACAGCTGTCGAAGTGGCCCAGCGCTGGAGGGAACTGGCTGAGAAGCTAGCCAAGGTC TCCAAGCAACAGATGGATGCCTATGAGTCTCCTCACCGAGACAGGAATGGGGTAGTAGACAGTGAGGCCA TGTGGAAACCCGCCTATGACTTCTTACTCACCTGGAGCCACCAGATTGGGGACAGCTACCGAGATGTCAT CCAGGAGTTGCACATAGGCCTGGACAAGATGAAGAACCCCATCACCAGGCGCTGGAAGCACCTCACGGGG ACACTGATCCTGGTGAACTCACTGGACATCCTGAGGGCAGCTGCCTTCAGTCCCGCGGACCATGATGACT TTGTGATCTGAGTGGGATCCCTGCCCTACTACTCACTGGAGTTGGGACCCCGCCCACCAGACCTGGGACT CCACCCACCAGAGCTTGGGACTCCACCCACCAGAGCTGGGACTTGGCCCATCATCTCTCCACAGTGGGCT GTGCCATCACCGGGTGGTGCAGGGAGGGATGGCTGCTCAGGTCGAGGCCTACCTGCCAGCCACGCCCAGG AGGGACTGAGGACTTTTGACTTTCTTCCCCTCCCCTCTATCCAGGGGCAGGTCCCACGGGCTTTCTACTT CCATTTGCCAATTGTGTTGCTTTCTATTTGTTGAAAATGTACGTTTAAATTTAAAATCACTTTTTTTAAC AGAAGACTTAGAAAGGACGTAAGAAAGGTGGTATCTAATTTTTTAATGGACTGTAAAGGTTATTTAAAAA TTTTTTCACCTTAGTAAAGGCAAATGCTATTGAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_133816
• Insert Size: 2889 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC012284, AAH12284
• RefSeq Size: 3688 bp
• RefSeq ORF: 2889 bp
• Locus ID: 98402
• UniProt ID: Q921I6
• Cytogenetics: 1 D
• Gene Summary: May function in transferrin receptor internalization at the plasma membrane through a cargo-specific control of clathrin-mediated endocytosis. Alternatively, may act as a negative regulator of the amino acid-induced TOR signaling by inhibiting the formation of active Rag GTPase complexes. Preferentially binds inactive Rag GTPase complexes and prevents their interaction with the mTORC1 complex inhibiting its relocalization to lysosomes and its activation. Thereby, may indirectly regulate cell growth, proliferation and autophagy (By similarity).[UniProtKB/Swiss-Prot Function]