Ezh1 (NM_007970) Mouse Untagged Clone

CAT#: MC205641

Ezh1 (untagged) - Mouse enhancer of zeste homolog 1 (Drosophila) (Ezh1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ezh1
• Synonyms: ENX-2
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC007135
  GGATGGAGGATCTTGCCATTCGTCTGCAGAACAGAGGTACATTATAAAGTCAACACTTCCCGCTGCATTC CATGAGGAAAATGGATATAGCAAGTCCCCCAACTTCCAAATGCATCACATACTGGAAAAGAAAAGTGAAA TCTGAGTATATGCGGCTTCGACAGCTCAAACGGCTCCAGGCAAATATGGGAGCAAAGGCTCTGTATGTGG CAAATTTTGCAAAGGTTCAAGAAAAAACCCAAATCCTCAATGAAGAGTGGAAGAAACTTCGTGTCCAGCC TGTTCAGCCAATGAAGCCCGTGAGTGGGCACCCTTTTCTGAAAAAGTGTACCATAGAGAGCATTTTCCCA GGGTTCGACAGCCAGGATATGTTGATGCGGTCTCTGAACACTGTTGCACTGGTTCCCATCATGTATTCCT GGTCCCCTCTCCAGCAGAATTTCATGGTGGAAGATGAGACGGTTTTGTGCAATATTCCATACATGGGTGA CGAGGTGAAGGAAGAAGATGAGACTTTCATCGAAGAGCTGATCAATAACTATGATGGCAAAGTCCACGGT GAAGAAGAGATGATCCCTGGATCTGTGCTGATCAGCGATGCTGTGTTTCTGGAGCTGGTGGATGCCCTCA ACCAGTACTCTGATGAGGAGGAGGACGGGCACAACGACCCCTCCGATGGAAAGCAAGACGACAGCAAAGA GGACCTGCCGGTAACAAGAAAACGGAAGCGCCATGCTATCGAAGGCAACAAAAAGAGTTCCAAGAAACAG TTTCCAAATGACATGATCTTCAGCGCCATTGCGTCCATGTTTCCTGAGAATGGTGTCCCTGACGACATGA AGGAGAGGTATCGAGAGCTGACAGAGATGTCAGACCCCAATGCACTTCCCCCTCAGTGCACACCCAACAT CGATGGCCCCAACGCCAAGTCAGTGCAGCGGGAGCAGTCTCTGCACTCTTTCCACACCCTTTTCTGCCGG CGCTGTTTTAAATATGACTGCTTCCTTCACCCTTTCCACGCCACCCCAAATGTATATAAGCGCAAGAACA AGGAAATCAAGATTGAGCCAGAACCGTGTGGCACAGACTGCTTCCTTTTGCTGGAAGGAGCAAAGGAGTA CGCCATGCTGCACAACCCTCGGTCCAAGTGCTCTGGGCGCCGCCGCCGAAGGCACCCAGTGGTCAGTGCT TCCTGCTCCAATGCATCAGCTTCTGCTATGGCTGAAACTAAAGAAGGAGACAGTGATAGAGACACTGGCA ATGACTGGGCCTCCAGTTCTTCAGAGGCTAACTCTCGCTGTCAGACCCCCACGAAACAGAAAGCCAGTCC AGCCCCAGCTCAGCTCTGTGTTGTGGAAGCCCCCTCAGAGCCGGTGGAATGGACCGGAGCCGAAGAATCT CTTTTCCGAGTCTTCCACGGCACCTATTTCAACAACTTCTGCTCAATAGCCAGGCTTCTGGGGACAAAGA CATGCAAGCAGGTCTTTCAGTTTGCAGTCAAAGAATCACTTATCCTAAAGCTGCCAACAGATGAGCTCAT GAACCCTGCACAGAAGAAGAAAAGAAAACACAGGTTGTGGGCCGCACACTGCAGGAAAATTCAGCTGAAG AAAGATAACAATTCTACACAAGTGTATAACTACCAACCCTGTGACCACCCAGACCGTCCGTGTGACAGCA CATGCCCCTGCATCATGACCCAGAACTTTTGTGAAAAGTTCTGCCAGTGCAGCCCAGACTGCCAGAATCG CTTTCCTGGTTGTCGCTGTAAGACTCAGTGCAATACCAAGCAATGTCCATGCTACTTGGCAGTTCGTGAG TGTGACCCTGACTTGTGCCTCACCTGTGGGGCCTCAGAGCACTGGGACTGTAAGGTGGTGTCTTGCAAAA ACTGCAGCATCCAGCGTGGCCTCAAAAAGCACCTGCTGCTGGCCCCTTCCGATGTGGCCGGATGGGGCAC CTTCATCAAGGAGTCTGTGCAGAAGAATGAATTCATTTCTGAATATTGTGGTGAGCTCATCTCTCAGGAT GAGGCTGATCGTCGAGGGAAGGTCTATGATAAATACATGTCTAGCTTCCTCTTCAACCTCAACAATGATT TTGTAGTGGATGCTACCCGGAAAGGAAACAAAATTCGCTTTGCAAACCATTCAGTGAACCCCAACTGTTA TGCCAAAGTGGTTATGGTGAATGGAGATCACCGCATTGGGATCTTTGCCAAGAGAGCAATTCAGGCTGGC GAAGAGCTCTTCTTTGATTATAGGTACAGCCAAGCTGATGCCCTCAAGTATGTGGGCATCGAGAGGGAAA CGGACGTCTTCTAGCCCTCTGGGCCCCGTGCCAGTGCTGTGGTGGCAGCACTGTCATGGCTTCAGGCACA CACCACTGCTGCTCCAGCTCCTGCAATGTCTTCCATGCTGAGAAACCCACCCACCCTGCATAACTAGGCC TCCACTTTATTCCAAGGGGACACACTGCCTCAGGAGAGGGGAAACAGAGGCGGTGAAGACCTGGTCTCCC AGGAGAGTTCAGATGTAAGAAGCTGCGCTCCCATGCCAGGAGGAGGAGATGGTGGGTGTGGGATAATGCC AGGGCTTGAGTTTTCCTCAGCTGCCAAGCCGGGGACCCTCAGGAACCAACCAGTGCTACCATTTTAGCTT TCTCGATCAAGAGTTCCATGTCACTGACTGTGCTCAGGGTTAAAGGTGAGCCTGGCAAGCATTGCAGACA GAACTCAAAGGGAGACATGTCTCCATGCCATCAAAGCAGGAATTTAAGGATACAGGAAGGAAAACTTAAG TTCTTTCATCCTGTGAACATAGAGCCAGCTAGACCCTTGGGAAAAGCCTACCCTATAGGTTACAGGGGTG GCAGGGTGGTTTTCAGGCTCCCAGGACATGTGCCAGTGACCCACTCCTTCCAAAGTTCTGGCTCAGTGGC AACATGCCTAAGCTGCGGCTATGCCTGGTTGGGAGTGAGTACTCGGGGTCAGTGAAGCTGCAGCTGTCCC TACCCTCCATTCCTCAGCGGCTTGTCCATGGAGCAGCTTGCTGCTTTCTTCAGGGTAGCTGGGCCTAATC TAGGCTAGAGGAAGATGTTGCTGGTCTGGTTTACTCCCTGTCTGTAGTACCTGCTGTTGGTTGTGTTTGT GGGTGTGTGGGGTGCCATTGCACATTTAGGAGTGTGTCACAGTGCAGGGTAGACTGCCAGGCAGACCACC ATTCTCTGGGATTCATAGAGAACAAAGCACTTTAACTCTCTTTTAGAAGGTTTATAGATTGGAGTGGAGT TTGAGCCAAGTCTCTGCCCGAATCCCACTTCTGAAGGAGGAAGAGGAGAGTGGAAGATCCTGCAGTTTAA TATCATTTTCTGAGAGGCAGGAGGAGCTGACTTGACACAAAAACAAAAACTAGTCAGCATAGCTACCTTG TGTCTGGTGTGCTGAGAATAGGGGCTTGTGCTGGAAGGGGCGTGGGTGGATACAGACTGCCCTAGAGTGT TAGAATGGGGCACAAGCTCCTTGCTTCCTGAAGAACCAGAGCTCCTGTGGCCCTTGCTAACCATTCCCTT CTCCCACACTGCCTCTTAGAGATTTGCATGGGACGTGCATGCTTGTGCACATACTCCTTCACTACACTGG CAGGTCCGTGTGTGCCTCACACTCAGCACACTCTACAGCTCCTCTCCCCACACCCAGTTCTGAAACTGAC ATCTGCTGGGGGGTTATGTCTATTGTGTGTATCAGGGATACCCTCCAGCCTAACGATAGGCTCTGAATTA CTCAGTCTCTGGGAAACCAGAGACTGGTGGAGGAGGGGCTGGTCATGAGGAATGCTTTAAAACATGTCGG TTCTAGACTGAGCTCAGCCTTCCCTCAGCAGGCATCACTGGCAGCCCGCTCCCTCCGCAGTTCAGGCCAA TAATACTCATGCGCTCCAGCCGCCCACTCTTGGTTCCTGCTGTCCTCTATTTTTAATGACGGGTTTCACC CCTCCTCTCCACTTCTGCCCCACGTCACGTGGTAGATAAAGTGAGGTGCTGTGGACTGGTGGGGAACTGG CGTACCTGCGGCTTAATTGCCAGTAAATTTCTTGCACTTTAAAGTCCTGTGGCTTGTGACCTCTTATCTA ATAAAGTGTTAAGACTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_007970
• Insert Size: 2253 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC007135, AAH07135
• RefSeq Size: 4179 bp
• RefSeq ORF: 2253 bp
• Locus ID: 14055
• UniProt ID: P70351
• Cytogenetics: 11 64.37 cM
• Gene Summary: This gene encodes a member of the Polycomb-group (PcG) family. The encoded protein is interchangeable with the related Enhancer of zeste 2 (Ezh2) protein as a core component of the polycomb repressive complex 2 (PRC2), which methylates histone H3 at lysine 27 and results in the transcriptional repression of affected target genes. This complex is involved in carrying out cell-fate decisions during embryonic stem cell differentiation. [provided by RefSeq, Sep 2014]