Tmem64 (NM_181401) Mouse Untagged Clone

CAT#: MC205542

Tmem64 (untagged) - Mouse transmembrane protein 64 (Tmem64), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Tmem64
• Synonyms: 9630015D15Rik; AI790744; AV300874
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC030341
  CCACGCGTCCGCGGCCGTTCCCTCTCCGCGAGAGGACGCAGGGAGCCGAGGCCTGGCGTGCGGGGTGCCA TGGAAGGCCGCAGGCGGGGGGACGCGGCGGGAGTGGAGACACTGGCCGGCTGAGGCCGAGGCATGCGGAG CCCCGGTGGGAGCCTGCCCCACACGCTGCCCCGGGCCTTGCAGCACGCCGGTCGGACGGGCGTCGTGGAG CAGCCGGGCCGCTGGGCACCGGAGCGGACAGCGGGAGGGGACCGCTCGGAGGACCGCCTTCCCCGCGGGG GCGGGGCCAGCGCGGCGGCGGCTGCTGCTGCGGCGGCGGCCTCGGGCGCCCTGCTCGGCGCCTATCTGGA GCGCCACGGTCTGCCCGCGGCCTCGGACTTGCCGGCGCCGGCCGGGGCGTTGGCAGGCGGCCCCGGGAGC GGCGGCGGCGTGGTGGTCGGGGTGGCCGAGGTGAGAAACTGGCGCTGCTGCTGCCTCGGCAGCACCTGTT GGTGCCGGAGCCTCGTGCTGGTGTGCGTGCTGGCCGCCCTGTGCTTCGCTTCCCTGGCCCTGGTCCGCCG CTACCTGCAGCACCTCCTGCTCTGGGTGGAGAGCCTCGACTCGCTGCTCGGTGTCCTGCTCTTCGTCGTG GGCTTCATCGTGGTCTCCTTCCCCTGCGGTTGGGGCTACATCGTGCTTAATGTGGCGGCCGGCTACCTGT ACGGCTTCGTGCTAGGCATGGGGCTCATGGTGGTGGGCGTCCTCATTGGCACCTTTATCGCTCATGTGGT CTGCAAGCGGCTACTCACCGCCTGGGTGGCTGCCAGGATCCAGAACAGCGACAAGCTGAGCGCCGTTATC CGCGTCGTGGAGGGAGGAAGCGGCCTGAAGGTGGTGGCGCTGGCCCGGCTGACTCCCATACCTTTTGGGC TTCAGAATGCAGTGTTTTCGATTACTGACGTCCCCTTGCCCAGCTACCTGATGGCGTCTTCAGCTGGGCT GCTACCCACTCAGCTGCTGAATTCTTACTTGGGGACCACACTACGGACTATGGAAGATGTCATCGCAGAA CAAAGTCTTAGTGGCTATTTTGTCTTTTGTTTACAGATTGTTATAAGCATTGGCCTCATGTTTTATGTAG TCCATCGCGCTCAAGTGGAATTGAATGCAGCTATTGTAGCTTGTGAGATGGAACTGAAAACCTCTCTGGT TAAAGGCAATCAATTGGATCCCAGTGGCTCTTCCTTCTACAACAAGAGGACCCTCACGTTTTCTGGAGGT GGAATCAATATTGTATGATTCTTACAAACTGTGATTGTTGAGAGCCTAGCGTGCTGTCCAGGCCTAGCAG TCACTTACCTGATGGCAGAGACAAGTGCTGTTGTATTATGGCACAAACAAAACTGACTAGTTTTTAAATT GCACAATTTTCTAAAAGCAAGAATCATTTTCTGGGTCTGTCAGTGTAAATGTAGATGCAGCTCTGGCTGC CCCCCCCCTTGTTATACCTGTAATGGCCTATAGGTGTGCACTTTAGTGTTTTTGAATTATTTTATTGCTG GGACTTATGAACAGAAAAATAACCCAAATATTTTCTTTCTGTTTAGTATCTTTATTTATAAAGCCCATAA ATAGTTGTGTGCATCTGTCTTTAAGGATGAGTGAAAGTATAGAGTTCGCAATGGCTCTTGTTGTATGCTC TTTGCTTAGGCATTCTCTCCAGAAGGGTGAACTGTTTTGTTTAATGTTCACTTGCTTGTTTCAAGCAGAA AAGCTCTGCTTTGTTTTCCTTGCTTGTAAGGAAATAGCTGTATTTCGGTTGACTGTATGTTATCCGGCAT TATGTCACTGTGGTCATTTACACCTCTCGGTACAAGTGTAAACCCTACATTGTGGGACTGAAGTGCTCTT AAGACCCCTTTTATTTTCACTGTGTAAGATGCAAAGTGAAAGGGACATTATTTAGCAGGTGGCTCACAAG TCGAGCCCGCATTCTTATTCGATTATATTGGTTTCTCCCATTAGACCTCATACAAGGGCTGTCCGATAGC AACTCTTACCAAACCATTTCCCCCTTTTATATTAATAATTAGGCTTTGAAATAAACTTATTGTTCCTATA AAATGGTGGGCTTACCATCTCTGACGTCATTCAGGTGGCCTTGTTTAGTCAAAACGCCTTGTTAAAAAGA AACGAGCTTTAAAATCATGTTTATAGTTTCAGTTAAAAAATATATATATGTCCCCATAGCTTTCAGCTTT AAAACTATAAACATTAACAAAAATCCCACATTCGTGCTGTCTGCTGCGTTATTGCTGTTTTGTTTGGGGC TTTGGTGTTCCTTTTCCCTGACAAGACTCAGGAATTCTGAAATGTGAAATTGTCTCAATCCTTTATCTTG TAGCATGTGTGTTTGCTAACAGCACTTAGGACTTAGCATACAATAATCCAACATGATTCGTATGGCGTGC ACTCATTTATCAGCTTTATTTTTAAAATAGTTTAATTGCAAACTTAATGATGAGTTAATTTTGTTGCAAA TAGATTGTAGTTGATACGGTAACGTTTTCTGTTAAGGACTTCACTTTTCTATGACTGTTCCAACCTATTA GAGCTCTCTCCGCTGCCTTTTGCTGGCAGTAAAACCTTTGCCCTGGGTACTGCTGACATCCAGAGAAATC CCGCGCAAGCAGATTTTTCTAGTCTTAACCCCAGAGAAGAGTTTGATTGTGTTCACACACGAGGTTTTTG CCCAGTTCAGGTGGCTTTCCTTCCCCTGCTGATGCGTAAATCCCTCTGCGTCTGAGTAGCAGTAGAAGAC CAGAGTAACTGGGACGAAAGGCACCTGGTCTACAGTGCATGCCTGGCCTCCAGACCTTTGTTATCGGTAG AAAAACAACTATACATACCGAGTCCTTACTGTCTGTTTAGTTAAGTTTCAGTTAGTTTAACCATCCCAAT AAAAGAAATATTTGATTTATTCTTTTTAGTACAACTTGGCAACATTTTTCTTGTGTTTCTCATTGGCTAT TTTTTGTTAAAGGAATCTTTATTTGAGATCATTATTTTCATGCTTGCTTTACAATTCATGTATTTTGAAG TGCATTATATTTACTTAGCATTTGTAACTTGAACGACTTCACCAAATGAATACCTTTTGGTAATTTGTAA TAACTTCTTCCAAATGTTAACACTTTCCCTGGTAATTGTATTACATATGTAAAGACAAAAGAAATACTTT TCTGTATTCTGCCAATCATAATTTTATATAATAAATCATCAACTTTTATAATAGTATGGATTGACAATTT CTAAAGTAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_181401
• Insert Size: 1146 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC030341, AAH30341
• RefSeq Size: 3314 bp
• RefSeq ORF: 1146 bp
• Locus ID: 100201
• UniProt ID: Q3U145
• Cytogenetics: 4 A2
• Gene Summary: Positively regulates TNFSF11-induced osteoclast differentiation. Acts as a regulator of TNFSF11-mediated Ca(2+) signaling pathways via its interaction with SERCA2 which is critical for the TNFSF11-induced CREB1 activation and mitochondrial ROS generation necessary for proper osteoclast generation. Association between TMEM64 and SERCA2 in the ER leads to cytosolic Ca (2+) spiking for activation of NFATC1 and production of mitochondrial ROS, thereby triggering Ca (2+) signaling cascades that promote osteoclast differentiation and activation (PubMed:23395171). Negatively regulates osteoblast differentiation and positively regulates adipocyte differentiation via modulation of the canonical Wnt signaling pathway. Mediates the switch in lineage commitment to osteogenesis rather than to adipogenesis in mesenchymal stem cells by negatively regulating the expression, activity and nuclear localization of CTNNB1 (PubMed:25979161).[UniProtKB/Swiss-Prot Function]