Vps18 (NM_172269) Mouse Untagged Clone

CAT#: MC205526

Vps18 (untagged) - Mouse vacuolar protein sorting 18 (yeast) (Vps18), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Vps18
• Synonyms: 9930024E13Rik
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC036129
  AGCCTATAATCTCTTGGGACTGGAGCTGGGGTGCAAAGCTGCGCCATGGTCTAGTTGGGGCTCCTCGATC GTCTCCTTCACAGAGAAAGCGAAAAGGGCCTCAGAATTTTTAAAAGGTTCTCAGGATCTGTCAGACGCTG GGGAAGCACAACTTGCAAATCCAGGACGACCGGGGTCCCAGTTGTGACCCCCAGCCCCCAGATCAGAGAT CCAGAACTTGATTGCCATGGCGTCCATCCTGGATGAGTACGAGGACTCATTGTCCCGCTCGGCCGTCTTG CAGACTGGTTGCCCTAGCGTGGGCATCCCCCATTCTGGGTATGTAAGTGCCCACCTGGAGAAGGAGGTGC CCATCTTCACCAAGCAGCGGGTTGACTTCACCCCCTCGGAGCGGATCACTAGCCTCGTGGTCTCCTGCAA TCAGCTCTGCATGAGCCTGGGCAAGGATACACTGCTCCGCATTGACTTGGGCAAGGCAAGTGAGCCCAAC CGTGTGGAACTGGGGCGCAAGGACGACGCCAAAGTCCACAAGATGTTCCTGGACCATACTGGCTCTCATC TGCTGGTTGCGCTGAGTAGCACCGAGGTCCTTTACATGAACCGCAATGGACAGAAGGCCCGGCCCCTGGC TCGCTGGAAGGGACAGCTGGTGGAGAGTGTGGGATGGAACAAGGCCATGGGCAACGAGAGCAGCACCGGC CCCATCCTGGTCGGCACAGCTCAAGGACAGATCTTTGAAGCAGAGCTCTCAGCTAGCGAGGGTGGCCTCT TTGGCCCTGCCCCAGATCTCTACTTCCGTCCACTGTATGTGTTAAATGAAGAAGGGGGTCCAGCCCCTGT GTGCTCCCTCGAGGCTGAGCGTGGCCCCGATGGCCGAGGCTTTGTCATTGCCACCACTCGGCAGCGCCTC TTCCAGTTCATAGGCCGAGCTGTGGAAGATACTGAAGCCCAGGGCTTCGCAGGACTCTTTGCTGCCTATA CAGACCACCCGCCCCCATTCCGTGAGTTTCCTAGCAACTTGGGGTATAGTGAGTTGGCTTTCTATACCCC TAAGTTACGCTCGGCACCTCGCGCCTTTGCCTGGATGATGGGAGATGGAGTGCTGTATGGCTCACTGGAC TGCGGGCGTCCTGACTCGCTGCTGAGTGAGGAGCGAGTGTGGGAATACCCAGCGGGGGTTGGTCCTGGGG CCAATCCACCCTTAGCCATCGTCCTGACCCAGTTCCATTTCCTACTGCTGCTGGCCGACCGGGTGGAGGC TGTGTGCACGCTAACAGGGCAGGTGGTGCTACGGGATCACTTCCTGGAGAAGTTTGGACCACTGAGGCAC ATGGTGAAGGACTCATCCACAGGCCACCTATGGGCCTACACTGAGCGTGCGGTCTTCCGCTACCATGTGC AACGTGAGGCACGGGATGTCTGGCGCACCTACTTGGACATGAACCGCTTTGACCTGGCCAAAGAGTATTG TAGAGAGCGGCCTGATTGCCTGGACACGGTCCTGGCCCGAGAGGCTGATTTCTGTTTTCGCCAGCATCGC TACCTGGAGAGCGCCCGCTGCTATGCGCTGACACAGAGCTATTTTGAGGAGATTGCCCTCAAGTTCTTGG AGGCCCGGCAAGAGGAGGCGCTGGCCGAGTTTCTCCAGCGGAAACTGGCCGGCTTGAAGCCGACGGAGCG TACCCAGGCCACACTGCTGACCACTTGGCTGACAGAGCTCTACCTGAGCCGCCTGGGTGCTCTGCAGGGT GACCCAGATGCTCTGACTCTCTACCGGGACACACGGGAGTGTTTCCGTACTTTTCTCAGTAGCCCTCGGC ACAAAGAGTGGCTCTTTGCTAGCCGGGCCTCTATCCACGAGCTGCTCGCCAGTCACGGAGACACAGAGCA CATGGTTTATTTTGCAGTGATCATGCAGGACTATGAACGGGTGGTCGCATACCACTGCCAGCATGAGGCT TACGAGGAGGCCCTGGCTGTGCTTGCCCGCCACCGGGACCCCCAGCTCTTCTACAAATTCTCCCCCATTC TCATCCGCCACATCCCCCGCCAGCTCGTAGACGCCTGGATTGAGATGGGCAGCCGGCTGGATGCTCGGCA GCTCATCCCTGCCCTGGTGAACTACAGCCAGGGCGGTGAGGCCCAGCAGGTGAGCCAGGCCATCCGCTAC ATGGAATTCTGCGTGAATGTGCTCGGTGAGACGGAGCAGGCCATTCACAACTACCTGCTGTCCCTGTATG CCCGTGGCCAGCCAGCCTCACTTCTGGCATACCTGGAACAAGCTGGGGCCAGCCCGCACCGTGTACATTA TGATCTCAAATATGCACTTCGACTTTGTGCTGAGCACGGCCACCACCGCGCCTGCGTCCATGTCTATAAG GTGTTGGAGCTATATGAGGAGGCTGTGGACCTGGCCCTGCAGGTGGATGTGGACCTGGCCAAGCAGTGTG CAGACTTGCCGGAGGAGGATGAGGAACTTCGCAAGAAACTATGGCTCAAGATCGCCCGGCACGTGGTGCA GGAGGAAGAAGACGTCCAGACAGCCATGGCCTGCTTGGCCAGCTGCCCCTTGCTCAAGATCGAGGATGTG CTACCCTTCTTCCCTGACTTTGTCACCATCGACCACTTCAAGGAGGCAATCTGTAGTTCCCTGAAGGCCT ACAACCACCACATCCAGGAGCTGCAGCGCGAGATGGAAGAAGCCACAGCCAGTGCCCAGCGCATCCGACG AGACTTGCAGGAGCTCCGAGGCCGCTATGGCACCGTGGAGCCCCAGGACAAATGCTCCACCTGTGACTTT CCTTTGCTCAACCGACCCTTTTACCTGTTTCTCTGTGGCCACATGTTTCACGCTGACTGTCTCCTACAGG CTGTGCGGCCCGGCCTCCCTGCCTACAAGCAGGCCAGACTTGAGGAACTGCAGCGCAAGCTTGGGGCGGC GCCTCCTCCGACCAAAGGCTCCGTGAAGGCCAAGGAGGCAGAAGCTGGGGCTGCAGCGGTGGGGCCCAGC CGGGAGCAGCTCAAGGCTGACCTGGATGAGCTGGTGGCTGCTGAGTGTGTGTACTGTGGGGAGCTGATGA TTCGGTCTATCGACCGGCCCTTCATTGATCCGCAGCGCTATGAGGAGGAGCACCTCAGCTGGCTATAGGA AGTTCCCCCGCATGGGTGGACAGACATGGATCCAGCTGCCAGACCCTCCTGCGAAAGCCAGGCCCTGGTT GGTGTTCGGTCCTTGAGCTTGCCGGGTCTGCGATTGTGAGGGGGTTGCAGCCAAGCTTCAGCCGGGAGGT TTTCAGGTGCGAACGCCGAGCTGTGCTTCAGACCTGTCTTAGACCTGCCCGTCCCAGGCAACCCGAGCTG GTGAGATCAGCCGTGATCCTTTTAACCACTACATCTAACAGTTCCTCTGTTCTCGTTAGCATGTTCACCT GGACTGAGTGGCCCGCTGTCCTTCCTTCCTCCGTCCTTCCACAGCCTCTGGACCTCCAGCTCGCCCTCTT CTGCCTCAACTAGGTGACCCAGGAGCAAGCAGGCAGACCCTAGGCACTTGTGGGGAGAGTGGGCATGTGG AGATGCTAATGTGACCTGGGGCTCCAGTGTCGGGTGCCCTGAGGCTGAGGGCACACCTGGGACAAGGGGA AATAGGGAGAGTATTAAATTGTGCTACAATGGTGGCCCTGGACTTGATGGCTTTGTGTTGATGCTGAAGC TAACAGGATATGATCCCAAGAAAGGATGGAATGCCACAAGAAGGCTGCCTGCACCAAGTGTGGAATAGAA CAGGTCAGGTCACGGCCAGCCTAGTCGACATAATAAGTTCTAGGCCAGCAAAGGCTACGTAGTATGACCC TGCCTGTCTCAAAAAAAAAAAAATTAAATTAAATTAAAAAAATAAAAATAAAAAGAAGAAAAAAAAAAAA AAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_172269
• Insert Size: 2922 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC036129, AAH36129
• RefSeq Size: 3924 bp
• RefSeq ORF: 2922 bp
• Locus ID: 228545
• UniProt ID: Q8R307
• Cytogenetics: 2 E5
• Gene Summary: Plays a role in vesicle-mediated protein trafficking to lysosomal compartments including the endocytic membrane transport and autophagic pathways. Believed to act as a core component of the putative HOPS and CORVET endosomal tethering complexes which are proposed to be involved in the Rab5-to-Rab7 endosome conversion probably implicating MON1A/B, and via binding SNAREs and SNARE complexes to mediate tethering and docking events during SNARE-mediated membrane fusion. The HOPS complex is proposed to be recruited to Rab7 on the late endosomal membrane and to regulate late endocytic, phagocytic and autophagic traffic towards lysosomes. The CORVET complex is proposed to function as a Rab5 effector to mediate early endosome fusion probably in specific endosome subpopulations (By similarity). Required for fusion of endosomes and autophagosomes with lysosomes (PubMed:14517315, PubMed:22854957). Involved in dendrite development of Pukinje cells (PubMed:22699122).[UniProtKB/Swiss-Prot Function]