Vsx2 (NM_007701) Mouse Untagged Clone

CAT#: MC205428

Vsx2 (untagged) - Mouse visual system homeobox 2 (Vsx2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Vsx2
• Synonyms: Chx10; Hox-1; Hox-10; Hox1; Hox10; or
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC031869
  CCTGGGACCAACTTCACGAAGCGGGGAGCCCGGCGGGGGGGCTTGGGGGAGCAAAAGACCCGCGGCCTCA GCCCCTCCAAAGAACCGGGAGATGACGGGGAAAGCGGGGGAAGCGCTGAGCAAGCCCAAATCCGAGACAG TGGCCAAGAGTACCTCGGGGGGCGCCCCGGCCAGGTGCACAGGGTTCGGCATCCAGGAGATCCTAGGCTT GAACAAGGAGCCTCCCAGCTCCCACCCGCGGGCTGCCCTCGACGGCCTGGCCCCTGGGCATTTGTTAGCT GCGCGCTCCGTTCTCAGCCCGGCAGGAGTGGGCAGCATGGGGCTGCTGGGGCCCGGGGGACTTCCCGGCT TCTACACACAGCCCACCTTCTTGGAAGTGCTGTCCGATCCGCAGAGCGTCCACTTGCAGCCACTAGGCAG AGCGTCGGGGCCGCTGGACACCAGCCAGACGGCCAGCTCCGATTCCGAAGATGTTTCCTCCAGTGACCGA AAAATGTCCAAATCGGCTTTAAACCAGACCAAGAAGCGTAAGAAGCGGCGACACAGGACAATCTTTACTT CCTACCAGCTAGAGGAGCTGGAGAAAGCATTCAATGAAGCCCACTACCCAGATGTCTACGCCCGGGAGAT GCTGGCCATGAAAACGGAGCTCCCAGAAGACAGGATACAGGTGTGGTTCCAGAACCGCAGAGCCAAGTGG AGGAAGAGGGAGAAGTGCTGGGGCCGGAGCAGCGTCATGGCTGAGTATGGTCTCTATGGAGCCATGGTGA GGCACTCGATCCCCCTGCCAGAGTCTATCCTCAAGTCTGCCAAGGATGGCATAATGGATTCCTGTGCCCC ATGGCTACTGGGGATGCACAAAAAGTCGCTGGAGGCAGCAGCTGAGTCGGGAAGGAAGCCCGAGGTGGAA CGCCAGGCCCTGCCCAAGCTCGACAAGATGGAGCAGGAGGAACGGGCCCCAGAGGCCCAGGCAGCCATCT CCCAGGAAGAACTGAGGGAGAACAGCATTGCCGCCCTCCGAGCCAAAGCTCAGGAACACAGCACCAAGGT TCTGGGGACTGTATCTGGGCCTGACAGCCTGGCCAGAAATGCTGAGAAGCCAGAAGAAGAGGACGCCACG GAGGAAGACAGGCCAGCTGAGAAGCTCAGCCCACCGCAGCTGGAGGACATGGCTTAGGGCAAGGGCTGCT GGCCTCGGAACTGCTCTTCTCCAGCCCTGCAGCCCTGCGGTGTGGCGAGTTCTCTCTGAGGCAAGGCCCA TGTCTGGCTTCTGCCACACCTCCCCACACCTCAGGCCCGCAATTACCCCCACGACTCTAGAAACAGACCA ATTCTGTCCGTAGCTTTGGACCATGCTGAGACATCCTGGACATACTCCCGATCCAGCGTCCTCAGCCTCA GCCCAGCTTCCCTGCCTCCAAAGCCTTCTTGCTACCCCGACATCGAGGTCCTGCTACGTCATCCCTGAGA GACTCTTGTCCAAGCCTATCCCTTATTCTCTGCTCTCTAAGCCTTCCTCCCCAGCTTAGCCCCCTCATGG GCGGCTGCGCCCACTGCTGTAAACCCAGCCCACAGCCGGGCAGAGCTGTGATTGGATTGAGGCCCTGCCT CGGTGTCCCGAGCACCCCAGCACTTTGCAGGCAGGCTTTTCCCTCTTCACCTGTTCCTTCTCGCAGACCT GGACCCTGGCTGGCTCCTGCTGTGTGAATGCACAGCCGGGTCACGTGGGTCCTGCTGTGAGGATGCACTG CTCAGCTGGTCCACGGTGTAACCTCTGCATTCTCTGCCCTGCCTGCTGATGGGCAGATTCAAGTCTAAAC AGGGCAACTCTCAGATTGAGTCCTTCCCCAAACCTGTCTCACGCCGTGCTGTAGATCATCCTCAGGCCTT CTCCGCACCGAGCCTGCCCTACATGTTCCTCCTTTGCAGAGGTGGGCCACACCACCTTGTCAGTGTCTAG GCCCTGCAGCCTGAGGGCGGGGCGCTTCTCGGCAATGCTGTGGCTTGCTTTACTGCTGGTGAGCCCTCAC CCAGAGCTTTTGCACCCCAGGGCCTGGGTGTCTGAAGCATTTTGACCCTTCTGCCTATGACTTTGACTTG CTAGTTAAGGCTTCTTTCTAGAGCTTCCTGTAGCCCAGTGGCTCTCAAGCTTCCTAATGCTATGACCTTT AATACAGTTCCTTGTGTTGTGGTGACCACCCCCCCCCAACCTTTAAATGTATTTCATTGCTACTTCATAA CTGTAATTTTGCTACTGTTATGAATCAAAATGTAAATATTTGATATGTGGAATATCTGATTATGTGACTT CCTCCAAAGGGGCCATGGGGGTCATGACCCACAGGTTGAGAACTGCTGTTGTAGCCCATTGGTTCACATG TGCCTGCTTTAGAGAAAATGGGTGCAGCTTAAAAGCCCCGGACATGGCCTCACTTTGACCTAGGCAGTCC TAAAAGGCCAAGCCAAAATCAGACCCACGCTATCTATCTATACACACACGCACACGCACACATGCACGCA TGCACGCACGCTGTATAACATGAAAGGTACGGGGAAAGCTCAAAGAACAAGGCAGGTAGCTAGTAAAAAA ACCAGACTAAGCTACAGACGCTCATAGCTCCGTGCCAGCTGTATGCCCAGAAAGGTGTCCATAGATAAGG AGCCATGTTGGACTGAATGCCTTCTGCCTGCAAAGGAGTGGCACTGTCCATACTGATGGCATGTCTTCAG ATGTCCCTGTCTGCAGATGTCTCTGTCTGCAGATGCCCCTTCCACCTGGCTTTATACATGTACTAGGGGT GCAGAGCCTTCTGGGGTCCTCTGCTCCTGTATTCCCAGGCCAACCACAAGACCCCCTTTATCCTGTGAAG TTACCCCACCTACCACTTCACAAAAAAGGCCCGGGCAGTGAGTTGTGATTTTAAATCTGTTTTGACAGTT GATTTCTTCTTCGTTCTCTTCATAGACTTCTCCCGCTTCTGAGTTCTTATCTTCTCCCGGGTTGAGATGT TGACTATAGTCCTGGTGTGTAAATAGTGTACATAATGTGAGAAGGAAGAAGCTGGACACTGAGGTGTTCG AAACACTGAATTGTCATAACTTTTAGGTATTCAAAGCATAATTTCTGTGCTGTTTTCTGTAAAGACACAA GCAAGAATAAAATTCATGCAAAGACTGCTAAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_007701
• Insert Size: 1086 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC031869, AAH31869
• RefSeq Size: 3197 bp
• RefSeq ORF: 1086 bp
• Locus ID: 12677
• UniProt ID: Q61412
• Cytogenetics: 12 39.28 cM
• Gene Summary: This gene encodes a member of the Vsx (visual system homeobox) family which belongs to the larger PRD homeobox class. The encoded protein is required for eye organogenesis and controls retinal development. Disruption of this gene is associated with ocular retardation J (orJ), a mouse disease which causes microphthalmia, retinal degeneration and optic nerve aplasia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2014]
  Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1.