Hacd3 (NM_021345) Mouse Untagged Clone

CAT#: MC205407

Ptplad1 (untagged) - Mouse protein tyrosine phosphatase-like A domain containing 1 (Ptplad1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Hacd3
• Synonyms: 4930523M17Rik; AW742319; B-ind1; Hcad3; Hspc121; Ptplad1
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC031755
  GCGCGAGCCGCGAGCGTGGGTTATCTCGAGACAGCCGGGCTGCGGGCTGCGGAGCGTGCGGGTCTGAGGC CTGCGTTCTGCTAGCTCCAGCGGCTCACAGGCCGAGGTAACGAGGCGCAGCAAGCCGAGCCTCCCCGCTC CCCCGGACAGCGTGGGCCATGGAGACTCAAGTGCTGACGCCGCATGTCTACTGGGCTCAGCGACACCGCG AGCTGTATCTGCGCGTGGAGCTGAGTGACGTGCAGAACCCTGCTATCAGCATCACAGACAATGTGCTGCA TTTCAAAGCTCAGGGACACGGTGCCAAAGGAGACAATGTCTATGAATTTCACCTGGAGTTCTTAGAGCTT GTGAAGCCAGAGCCGGCGTACAGGCTGACCCAGAGGCAGGTGAACATCACAGTCCAGAAGAAGGGGAGTC ATTGGTGGGAAAGGCTCACCAAGCAAGAGAAGCGCCCATTGTTTTTGGCCCCTGACTTTGATCGCTGGCT GGATGAATCTGATGCGGAAATGGAGCTGAGAGCCAAGGAGGAAGAACGCCTAAATAAACTCAGACTAGAA AGGGAAGGCTCCCCTGAAACTCTTACAAACTTGAAGAAAGGGTACCTGTTCATGTACAACCTTGTGCAGC TCCTGGGGTTCTCCTGGATCTTTGTCAACCTCACAGTGCGGTTCTTTATTTTAGGAAAAGAGTCCTTCTA TGACACGTTCCACAATGTGGCTGACATGATGTATTTCTGCCAGATGCTGGCACTAGTGGAAACCCTCAAT GCTGCGATTGGGGTCACAAGTACCCCGGTGCTGCCTGCTCTCATCCAGTTTCTTGGAAGAAATTTTATTC TGTTTCTTGTTTTCGGCACCATGGAAGAAATGCAAAACAAAGCTGTGGTTTTCTTTGTGTTTTATTCCTG GAGCGCAATTGAAATCTTCAGGTATCCCTTCTACATGCTCTCCTGCATTGACATGGACTGGAAGGTGCTC ACGTGGCTCCGTTACACTATGTGGATTCCATTATATCCCCTGGGATGTTTGTCAGAAGCTGTGGCAGTGA TCCAGTCCATTCCAGTATTCAACGAGTCGGGAAGGTTCAGTTTTACTTTGCCATATCCAGTGAAGATGAA AGTCAGATTTTCCTTCTTCCTTCAAGTTTATCTCGTAATGTTATTTTTAGGGTTATATATTAATTTTCGT CACCTTTATAAACAGCGCAGGAGGCGGTATGGACAAAAGAAGAAAAAGCTCCACTAAAGATGAATTTGGT AGCTTCATTTGAGCAGAGTCTATGATTCCTACTCTTCTACTTCTTGTACTGATATAAGGGTTTGTTTTGT TTTGTTTTTTAAAGTAAGGCTGTCTCTCTTTCCCAGTAACATTCCTGAGTCTACTGTCCTTATCTTAGTG TTGTACTTGCATGACATGGATATCTGGTATCTGAGGACAAGTTCATTCTTGTGTATTTAATGACAAAGGT GGCCCTCTTCATGTCTGCTCCTCCCTACAGGCACTGTCTGTCTTCACAGGGCTTGGTCTTTCTGCTGCCA CTTGTGAAAACCGAAAAAAAAACAAGACTACATAGCACGGACTTTTTGAAAATCAGATAAACATGGTTAT TGACATTTTTTGAGTCAGAGTAGGGCTGGAGAACACTTCATATTTGATGCATTTTAGCTAAAGACATTCA TTACTATAGCACTTCCCAGGCATTGAAGAGGCAGAGTGATTTAGAGAACGAAATCAGAACCTGGGAACAC CATGACTACTTCATTTCTAGCTAAATCAGAACTGCCTTCAACACTGGAGAAAGGGAGAAGTACTGCGCAA CAGAGATTAGAAGTCCAAATGTGAATGGCTGCAGGGGTCAGAAGGGAACAGAGATGCAGGAGACAGGCAG GATCAGTTGTGGTAATGATGCCTGTGGCAAACACAAACACCTACTTGGGCTGCACACAGTCACACAATCA ATCTATAATTCTTGGGGTTAGCTCAGTCCATTTACTTGTGTATTTCTGAGTGGTTGGTGAGATTACTTTA TTGAACTGTTTTTTTCCTTGTTAGATTTTTTTTTCTCTTTTTTTGTTTTTTAAGATAGGGTTGTTCTGTG TAGCTTTGGCTCTCCTAGAACTTGCTCTGTAGACCAGGCTGGCCTTGAACACAGAAAGAGATTCATCTGC CTCTGCCTCCTGAGTGCTGGGATTAAAGGCAAAAGCCACCACAGCCTGTTTTGTTTTGTTTTCGGTTTAA AAACAACAACAAACTGTGTGCATGCAGAATACAGACCTTGAGGTTGGCTACTGTTCAAGACAGCTTCAGT CTCTCTACCACATTCCATGTAAACCTATGGTGAAGTAACACCAGAAAAAAGAGCCTGAGAGTGGTCGCTT CCATTTTCCACTCCCCTTGTTCTTGGGGGGAAAGGAAGGAGACTTCTGCTGTTGCAGTGAGCCCAGAGTG GATTGGCATTAAACTGGCTGCTTTGACACACCTCATCTCACCCAAGAGTATTTCCCAAGTCTGCAGCTTC TGTCAGCGATTGTTGTGTTACCTTCTGGTGTTCCCTCACTGATTGTGGACAAACCTGGCAGTTGAGCTTT GCAGTCAATTATGCCGTATCCCTGTGCAAACTCCCATGTTCTAGTTGCTGTTAGCATCCCCTTTGTTGCT GTCTGAGAAAGTGAAAGATTGTGTATTTCTATTAAAACATTTACAGTCAAAATCCTAAAAAAAAAAAAAA AAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_021345
• Insert Size: 1089 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC031755, AAH31755
• RefSeq Size: 2733 bp
• RefSeq ORF: 1089 bp
• Locus ID: 57874
• UniProt ID: Q8K2C9
• Cytogenetics: 9 C
• Gene Summary: Catalyzes the third of the four reactions of the long-chain fatty acids elongation cycle. This endoplasmic reticulum-bound enzymatic process, allows the addition of two carbons to the chain of long- and very long-chain fatty acids/VLCFAs per cycle. This enzyme catalyzes the dehydration of the 3-hydroxyacyl-CoA intermediate into trans-2,3-enoyl-CoA, within each cycle of fatty acid elongation. Thereby, it participates in the production of VLCFAs of different chain lengths that are involved in multiple biological processes as precursors of membrane lipids and lipid mediators. Involved in Rac1-signaling pathways leading to the modulation of gene expression. Promotes insulin receptor/INSR autophosphorylation and is involved in INSR internalization (By similarity).[UniProtKB/Swiss-Prot Function]