Enah (NM_001083121) Mouse Untagged Clone

CAT#: MC205302

Enah (untagged) - Mouse enabled homolog (Drosophila) (Enah), transcript variant 4, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Enah
• Synonyms: Mena; NDPP-1; Ndpp1; WBP8
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC062927
  CGCCTGAGAAGAGACCCTCCGCTCGGCGGCTCCCGCGCCGGGGAAGCCTCGGCGCCGCCGGCACCATGAG TGAACAGAGTATCTGTCAGGCAAGAGCTGCTGTGATGGTCTATGATGATGCCAATAAGAAGTGGGTGCCA GCTGGTGGCTCAACTGGGTTCAGCAGAGTACATATATATCACCATACAGGCAACAACACATTCAGAGTTG TGGGCAGAAAGATTCAAGACCATCAGGTTGTGATAAACTGTGCCATTCCTAAAGGGCTGAAGTACAATCA AGCTACACAGACTTTCCACCAATGGAGGGATGCTAGACAGGTGTATGGTCTCAACTTTGGCAGCAAAGAG GATGCCAATGTCTTCGCAAGTGCCATGATGCATGCCTTAGAAGTGTTAAATTCACAGGAAGCAGGGCCAA CATTGCCTAGACAAAATTCACAGCTACCTGCTCAAGTTCAAAATGGCCCATCCCAAGAAGAGCTGGAAAT CCAGAGAAGGCAACTGCAAGAACAGCAGCGACAGAAGGAACTGGAGAGGGAAAGAATGGAGAGGGAAAGG TTGGAGAGAGAACGACTAGAACGAGAGAGGCTAGAGAGGGAGCGCCTGGAACAAGAGCAGCTGGAGCGGC AGCGGCAGGAAAGGGAGCACGTGGAGCGGCTGGAGAGGGAGAGGCTGGAGCGCCTGGAGCGAGAGAGGCA GGAGCGGGAGCGAGAGCGCCTGGAGCAGCTGGAGCGGGAGCAAGTGGAGTGGGAGCGAGAGCGCAGAATG TCCAATGCTGCTGCCCCTGCCTCTGCGGAGACCCCTCTAAATCCTGAGCTGGGAGACTCCTCTGCTTCCG AGCCAGGCTTGCAGGCAGCCTCTCAGCCGGCCGAGTCGCCAACCCCACAGGGCCTTGTCTTGGGACCACC TGCACCTCCGCCACCACCCCCTCTCCCATCAGGCCCTGCCTACGCCTCAGCACTTCCTCCTCCCCCAGGA CCCCCTCCACCACCTCCACTGCCATCCACTGGTCCTCCTCCTCCACCCCCTCCACCACCCCCTCTTCCTA ATCAAGCTCCTCCCCCTCCTCCCCCACCTCCTGCCCCTCCCCTCCCCGCATCTGGAATTTTCTCTGGATC CACGTCAGAAGACAATCGCCCTTTAACTGGACTTGCAGCTGCAATTGCGGGAGCAAAACTTAGGAAAGTG TCCCGGGTGGAGGATGGCTCTTTCCCAGGTGGAGGGAATACTGGGAGTGTGAGCTTGGCCTCATCCAAAG CAGACGCTGGGCGTGGGAATGGACCTCTTCCTCTAGGGGGTAGTGGCTTAATGGAAGAAATGAGTGCCCT GCTGGCCAGGAGGAGAAGAATTGCTGAGAAGGGATCAACAATAGAAACAGAACAAAAGGAAGACAGAAAT GAAGATGCAGAGCCTATAACTGCTAAGGCCCCATCAACAAGTACACCTGAACCAACCAGAAAACCTTGGG AAAGAACAAACACAATGAACGGCAGTAAGTCACCTGTCATCTCCAGACCCAAATCCACACCTTCATCACA GCCAAGTGCCAATGGAGTCCAGACAGAAGGCCTTGACTATGACAGGCTGAAGCAGGACATTTTAGATGAG ATGAGAAAAGAGCTGGCAAAGCTGAAGGAGGAGCTTATTGACGCAATCAGGCAGGAGCTGAGCAAGTCGA ACACTGCATAAAGAAGCAAACTACGGAGGGGCAGGACTTGAATCTGGAGAAAACAAAAATTCCTACAAAC AACTCTTAACCCCCAAACTTTTAAGCTGTAAGAAGAAAATGGATACACAGTCAGGAGGGAAGCCGTCAAC CTCTGAAAGCCTCAGACAGTGACTCTGGCGATCAGCTGTCCCCTCAGTGTGCTGCTTTATTCTGTCTGAC CTTTACCACAGGATGGAGAATGATATTGGAGTTCCCTTAGCAGTACTAAACCCGTCAGGCAAGATCACCG TGCATTGAAATATTTTCATGTCTAGATGAGTCTGCACGTTTTCCATAATCCATTGCTAAAATAAAGACGA GAAAGGCTTCAGAGATTTTTTTCCAGAGGGCGCTGATGAAGAATTTGATTTAGTAAGTTCTGCTGTTGCG TTGTAAATGTTTCTCTCAGGTTTGTCTTCCTATCATATTTGATATTCCGTGAACACTAAGATCATAAAGG ATGGAACAAACGGAGTTGTATGTGCTTTCAGAGGGTGGTGTTTAATACGAAAGGTCCCAAAATGTTTAGT CCCACTTGAGGAATTTTAGAATGATAGGAAGTCTCTCGGGTTAGCCTTCATGCAATTTTGTAGTTTAAAA CATAAAGTCCGTCCAGAAATTAAAGATTTAGATGCCTTCCTAAATTGTTACAATGCTTTACCAAATCTAT GACTTCTACATAACCAACAGTGGTCAAATGGAAAACATTCTATTATAGATTTACTGTAGGTTTTCAACCT TTTTTAGACATATGCATGTGGACATTTTTATAATGTAATTACAATCACCACAAAGTTAGCTTTTTTAATT ACAGACAATAATGCATGTCACACTAATATGTAGTGGCCTTTTCAGGGCCTAGTCCCAGGGAAAATATTTT GTAGAATATAGGGAAGTGGGAGGAAGGGAAGGAATAACTTTTTATTTAAAGTTAATTTCTGCACTATCTT TTTTTTCTCAATTATCTGCATGAATTATAATGAAAAATATTTTGTGACTTTAATCAGTAAACATGTTAAC AAAACCAAGTACTTAATCTTTTACATCATGTCTTCAGCTATTTGTATTTTAACCAGCAATTTCAACGGTC TGAAACATGATTCTGAGCGTAACATGTGAGTCCGTCTCACTGTGGAACTCACGAGTTTGATCACTGAATT TGGCAGTTATTTTCACGCGGCCCCCACAGTGCTGCAGTGCTTGGGTTCACGCTCCTGACTCTGCGGCTGC TCCTTGACCTTTGCTGTGCTAAAATACAAGAAATATCAATAATGGCAGCAACCAGGGTCACATAAGTCAC TAGGTAAGGGAGACCAATGAGGAGTTCTGCAGCTTTCTTTTAATAGAACTAAAGTGAGGCTTAAGTGGTA GGGAGTGTCTGCTGTTACACACACACACGCACACAGGTGAGAGAGAGAGAGAAAGTGATCGAGCTTAAGA GTGTGTGATAGATATTATGTTCCACCCCTTTATAATTGGCAAGATTAAAATAAGGGAGAAAATTTTATAT GTTGTAATGTTAGCTTTTTGGAAAATCTAGGAAATCAAAAATTCTCCAGGCTGTCCATCATGATTTATGC TTGAGTTATTAATGTGCCATATTTGCTTTGGAATCTTTGGCTATCAAAACTTTTGCTAAAAAAATAGTAG ATACAATCCACTTTGATTTGCTTTCTAGATTTCCTATGTCTCAGCTGTAAAGCAAAGCTTGATGAGAGTA GTTTTCTAAACCCTGCAGTTTGCAGCTAACCCCACTGCAGAGCCGTGTGAATGAGGGGTTTTGTTAAGAG TGTTTGTTTCTGTAGAAACTTCATCTTTAGATTAAAGTGTGAGGGATGAGCGGTCATAAGTCAATTCTGT GGCTTTCTCTACCCATCAGTCGTCGTCTCGCAGGAATAGTAAAGGCATTAAGGATGCGGCAAGCATGTAC GCTGTTCTAAAAGAAGTATGCATCTCATTTACTGTTGAACGTTTGATTACGCAACTACTTTGATTGTGTA CGTGTGTACATCCCTTACAGATCTGATCAAAAATCGCTTTTCATTTTGTTGCTAATGTTAAATAGTCTGT AGTACGTAGAGTACTGGGTCTAGATGGTCCAAAGTGAGACAAAAATGCTGAAGTCAAATGCTAAGTCCTA AACAAAGCTGGTTTATGCACTAAACGTTTTGTGCCTTGGTCTAATATTTAACACAATGTCTGTAAAAATG ACAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: AscI-NotI
• ACCN: NM_001083121
• Insert Size: 1626 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC062927, AAH62927
• RefSeq Size: 3942 bp
• RefSeq ORF: 1626 bp
• Locus ID: 13800
• UniProt ID: Q03173
• Cytogenetics: 1 84.93 cM
• Gene Summary: Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance and lamellipodial and filopodial dynamics in migrating cells. ENAH induces the formation of F-actin rich outgrowths in fibroblasts. Acts synergistically with BAIAP2-alpha and downstream of NTN1 to promote filipodia formation.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (4) lacks an alternate in-frame exon in the 5' coding region and uses an alternate in-frame splice site in the 3' coding region compared to variant 1. The encoded protein (isoform 4) is shorter than isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.