Kif5a (NM_008447) Mouse Untagged Clone

CAT#: MC205289

Kif5a (untagged) - Mouse kinesin family member 5A (Kif5a), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Kif5a
• Synonyms: D10Bwg0738e; Khc; Kif5; Kns; mKIAA4086
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC058396
  CCCGCATCCAGTGCCGCAGGACACCGAAAGCTCATCTTCCCCGAGGCGTTCCAGCTGCTCCCTTGGCCTC AGCGACAGATCACCTGTCCACTGCCTCGACCTCAGCCCAGCGTCCCCTTCCTCCGGAGTACACGCTACCC TTGCAGTCGGAGACGCTCCCAGCCCCTCGTCGTCGTCGACCACCATGGCGGAGACTAACAACGAATGCAG CATCAAGGTGCTTTGCCGATTTCGGCCCCTGAACCAGGCCGAGATTCTGCGGGGGGACAAGTTCATCCCC ATTTTCCAAGGGGACGACAGCGTCATTATTGGGGGAAAGCCATATGTCTTTGACCGCGTCTTCCCCCCAA ACACCACTCAGGAGCAGGTTTACCACGCCTGTGCCATGCAGATCGTCAAAGACGTCCTTGCTGGTTACAA TGGCACAATCTTCGCTTATGGACAGACATCCTCAGGGAAAACGCATACCATGGAGGGGAAGCTGCACGAC CCTCAGCTGATGGGCATCATTCCCCGGATCGCTCGAGACATCTTCAACCACATCTACTCCATGGATGAGA ACCTTGAATTCCACATTAAGGTATCTTACTTCGAGATTTACCTGGATAAGATCCGTGACCTTTTGGATGT GACCAAGACGAACCTGTCCGTGCATGAGGACAAAAACCGGGTGCCGTTTGTCAAGGGTTGTACCGAACGC TTTGTGTCCAGCCCAGAGGAGATTCTGGATGTGATCGATGAGGGGAAGTCCAACCGTCACGTAGCTGTCA CCAACATGAACGAGCACAGTTCTCGGAGCCACAGCATCTTCCTCATCAACATCAAGCAGGAGAACGTAGA GACCGAGCAGAAGCTCAGCGGGAAGCTGTACCTCGTGGATCTGGCCGGAAGCGAGAAGGTCAGCAAGACA GGGGCAGAGGGAGCCGTTCTGGACGAGGCAAAGAATATCAACAAGTCGCTGTCGGCCCTGGGGAACGTGA TCTCTGCACTGGCAGAGGGCACCAAAAGCTACGTGCCGTACCGCGACAGCAAAATGACGAGGATTCTCCA GGACTCTCTGGGAGGGAACTGCAGGACTACCATGTTCATCTGCTGCTCGCCGTCCAGCTACAATGACGCA GAGACCAAGTCCACGCTCATGTTTGGACAGCGGGCGAAGACCATCAAGAACACTGCCTCAGTGAATCTGG AGCTGACTGCTGAGCAGTGGAAGAAGAAGTATGAGAAGGAGAAGGAGAAGACCAAGGCCCAGAAGGAGAC AATTGCGAAGCTAGAGGCTGAGCTTAGCCGGTGGCGCAATGGAGAGAATGTGCCTGAGACTGAGCGCCTG GCTGGAGAGGACTCAGCTCTGGGAGCTGAGCTCTGCGAGGAGACCCCTGTGAATGACAACTCATCCATTG TGGTACGCATCGCACCTGAGGAAAGGCAGAAATATGAGGAAGAGATCCGCCGTCTCTACAAGCAGCTTGA TGACAAGGATGATGAGATCAACCAGCAGAGCCAGCTCATTGAGAAGCTGAAGCAGCAGATGCTGGACCAG GAAGAGCTGCTCGTGTCCACTCGGGGAGACAACGAGAAGGTCCAGCGGGAGCTTAGCCACCTGCAGTCCG AGAACGATGCTGCGAAGGACGAGGTGAAGGAAGTGCTGCAGGCCCTAGAGGAGCTGGCGGTCAACTACGA CCAGAAGTCCCAGGAGGTGGAGGAGAAGAGCCAGCAGAACCAGCTGCTGGTGGACGAGCTGTCCCAGAAA GTGGCCACCATGCTGTCCCTGGAGTCCGAGCTACAGCGGCTCCAGGAGGTCAGTGGACACCAGCGAAAGC GGATCGCTGAGGTGCTGAATGGGCTGATGAGGGACCTGAGTGAGTTCAGTGTCATCGTGGGCAACGGCGA GATTAAGCTGCCCGTGGAGATCAGTGGGGCCATCGAGGAGGAGTTCACGGTGGCCCGGCTCTACATCAGC AAGATCAAGTCGGAGGTGAAGTCCGTGGTTAAGCGATGTCGGCAGCTGGAGAACCTCCAGGTGGAGTGTC ATCGCAAGATGGAGGTGACCGGTAGGGAGCTGTCATCTTGCCAACTGCTCATCTCACAGCATGAGGCCAA GATCCGTTCACTCACGGAGTACATGCAGACTGTGGAGTTGAAGAAACGGCACCTGGAAGAGTCCTACGAC TCCCTGAGCGATGAGCTTGCCAGGCTCCAGGCGCACGAAACTGTACACGAGGTAGCTCTGAAAGACAAGG AGCCAGACACACAGGACGCGGAGGAGGTGAAGAAGGCCCTGGAACTACAGATGGAGAATCACCGTGAGGC CCATCACCGGCAGCTGGCCCGCCTCCGGGATGAGATTAATGAGAAACAGAAAACCATTGATGAGCTGAAA GACCTGAACCAGAAGCTCCAGTTAGAGCTGGAGAAGCTTCAGGCCGACTATGAGAGGCTGAAGAATGAAG AGAACGAGAAGAGCGCCAAGCTCCAGGAGCTGACATTTCTGTATGAGCGACATGAGCAGTCCAAGCAGGA CCTCAAGGGGCTGGAGGAGACAGTTGCCCGTGAACTCCAGACCCTCCACAACCTTCGCAAGCTGTTCGTT CAAGACGTCACGACTCGAGTCAAGAAAAGTGCAGAAATGGAGCCCGAGGACAGTGGGGGGATTCATTCCC AAAAGCAGAAGATCTCCTTTCTTGAGAACAACCTGGAACAGCTTACAAAGGTTCACAAACAGCTGGTACG TGACAATGCAGATCTGCGTTGTGAGCTTCCTAAATTGGAAAAACGACTTCGGGCTACGGCTGAGAGAGTT AAGGCCCTGGAGGGTGCACTGAAGGAGGCCAAGGAGGGCGCTATGAAGGACAAGCGTAGATACCAGCAGG AGGTGGACCGCATCAAAGAAGCCGTGCGGTACAAGAGCTCCGGCAAGCGGGGCCATTCTGCCCAGATCGC TAAGCCTGTGAGGCCTGGCCACTATCCTGCCTCCTCACCCACCAACCCCTACGGCACCCGGAGCCCCGAG TGTATCAGCTACACCAACAACCTCTTCCAGAACTACCAGAACCTGCACCTGCAGGCTGCGCCTAGCTCCA CTTCAGATATGTACTTTGCCAGCAGCGGAGCCACATCTGTTGCCCCCTTGGCTTCCTACCAGAAGGCCAA CATGGACAATGGAAATGCCACAGATATCAACGACAACAGGAGTGACCTGCCGTGTGGCTATGAGGCTGAG GACCAGGCCAAGCTTTTCCCTCTCCACCAAGAGACAGCAGCCAGCTAACTCCCAACCCTGGCCACATATC TGCACTTTCAGGTAGCTGCAGGCCACTTCCCTCCCTAGCCCCAGCCTCCTCCTGTCTGGCCATGGCTGCT TCTCCTGTTCATGTGTATGTGTATATGTTTGTATGTGTCTGTATGTGTGTGTTGTGTGTGCATGAGTGTA CCCACATCAGGAGGCCAAGGTTAGTAGGTGTGGGTGTCTTCATCTGTGTGTGTGTGTGTGTGCGCGCTCA CATCCACAGGCCAGCCTTGGTGTGGGTGTCTTCCTCTCTCACTTTCCATCTTACTTTTTGAGGTCTCTCA CAGAACCTGGAGCTTGCTGTTTTGGGGTAGACTGGCTGGTCAGTCACCTCCCCACCCTCCCTCTGTCCCA CCCCACCCCCCATATCTGCCCATTTCTGTCCCCTGGGGTACTGGGGATCCAAACATAGTCCTCATGTCTG TTCAGTAAGCACTTAGTGACTGAGCCAAAATCTTCCAACTCCTGCTTGGCCCTGTATGGCTGCACGAGGC AGAGGCAGGGAGATCATGAGTTCAAAGGAGTCCTGGATTCATGAGACCCTGTATCAAAACCAAACAGAAA CATTTGCTAAAAAGCCCAAATCTCATTCATAGCCAACCCTGACCTCCCATGGCCCCAAGCCTTCCCTTAG GTGCTGGTGGTAATCACAGTACTCATTTCTACCACTAGGGGTCACTGTCATACATTAAAATTTTTGTTAA AGGCAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: AscI-NotI
• ACCN: NM_008447
• Insert Size: 3084 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC058396, AAH58396
• RefSeq Size: 4021 bp
• RefSeq ORF: 3084 bp
• Locus ID: 16572
• UniProt ID: P33175
• Cytogenetics: 10 74.5 cM
• Gene Summary: Microtubule-dependent motor required for slow axonal transport of neurofilament proteins (NFH, NFM and NFL) (PubMed:12682084). Can induce formation of neurite-like membrane protrusions in non-neuronal cells in a ZFYVE27-dependent manner. The ZFYVE27-KIF5A complex contributes to the vesicular transport of VAPA, VAPB, SURF4, RAB11A, RAB11B and RTN3 proteins in neurons (PubMed:21976701). Required for anterograde axonal transportation of MAPK8IP3/JIP3 which is essential for MAPK8IP3/JIP3 function in axon elongation (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 3' UTR compared to variant 1. Variants 1 and 2 encode the same protein.