Iars (NM_172015) Mouse Untagged Clone

CAT#: MC205234

Iars (untagged) - Mouse isoleucine-tRNA synthetase (Iars), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Iars
• Synonyms: 2510016L12Rik; AI327140; AU044614; E430001P04Rik; ILRS
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC067029
  CACCTCCAGACGCGGAACGTGGTCGCAGGCTGCAACCTGAGCGAGCTCTCCGGGTTCCCAGCAACAGAAT GGTTCAACAAGTTCCAGAAAACATCAGTTTTCCTGCTGAAGAAGAAAAAATCTTGGAGTTCTGGTCCAAA CATAACTGTTTCCAGGAATGCCTCAAACAGTCAAAACTCAGGCCAAAGTTTACCTTCTATGATGGGCCTC CCTTTGCTACTGGACTGCCTCACTATGGACACATACTTGCTGGGACAATCAAAGATATCGTTACGAGATA CGCTCACCAGAGCGGGTTTCACGTTGACAGAAGATTCGGGTGGGACTGTCACGGCTTACCTGTGGAGTAC GAAATCGATAAGACACTGGGCATCAAAGGCCCGGAGGATGTGGCCAAAATGGGGATTGCAGAGTACAACA AGCAGTGCCGAGCAATCGTGATGAGATACTCCGCTGAGTGGAAGTCTACTGTTACCAGACTCGGCCGATG GATCGACTTTGACAATGACTACAAAACACTATACCCACAGTTCATGGAATCAGTCTGGTGGGTCTTCAAG CAGCTGTATGACAAAGGCCTCGTGTACAGAGGCGTGAAGGTCATGCCCTTCTCCACCGCGTGTGGCACTC CACTCTCCAACTTCGAATCTAACCAGAATTACAAGGATGTTCAGGATCCCTCAGTATTTGTAACATTCCC TTTGGAGGAAGATGAAAATACATCCTTAGTTGCTTGGACAACCACTCCTTGGACTCTACCCAGTAACCTT GCACTATGCGTCAATCCAGAAATACAGTATGTGAAGATCAAAGATGTGGCCAGGGGAAAATTATTCATCT TAACGGAAGCCAGATTATCGGCCCTCTATAAGCAAGAGAGCGACTATGAGATACTTGAAAGGTTTCCTGG TGCCTCTCTCAAAGGCAAGAAATACAAGCCGTTGTTTGACTACTTTATTAAGTGTAAGGAGAACGGTGCC TTCACCGTGCTTGTCGACCACTATGTGAAGGATGAAGAAGGCACAGGCGTTGTGCACCAGGCTCCTTACT TTGGTGCTGATGATCATCGTGTCTGTATGGACTTCAACATTATTCAGAAAGACTCAGTCCCTGTTTGTCC TGTGGATGCTTCAGGCTGCTTCACAGAGGAAGTGACACATTTTGTGGGGCAGTATGTGAAGGATGCTGAC AAAAATATCATTAGGATGTTGAAGGAGCAGGGCCGGCTGCTGGCGGCGGGCACGTTCACTCACAGCTACC CTTTCTGCTGGAGGTCAGACACGCCCCTCATTTACAAGTCTGTGCCCAGCTGGTTTGTGCGGGTGGAGCC CATGGTGGACCAGCTACTGAAGAACAACGACCTGTGCTACTGGGTCCCAGAGTTTGTACGAGAAAAACGG TTTGGAAATTGGCTGAAGGAGGCCCGTGACTGGGCAATTTCCAGAAACAGATACTGGGGCACCCCCATCC CACTGTGGGTCAGCGAAGACTTGGAGGAGGTAGTGTGCATTGGATCAGTGGCAGAGCTTGAAGAACTGTC GGGAACAAAAATCTCAGATCTCCACAGAGAGAGCATCGACCACCTGACCATCCCATCCCGCTGCGGGAAA GCACCATTGCGGCGCGTCTCTGAGGTGTTTGACTGCTGGTTCGAGAGTGGAAGTATGCCCTATGCCCAGG TCCATTATCCGTTCGAGAGCAAGCGCGAGTTTGAGGATGCCTTTCCTGCAGACTTCATTGCTGAAGGCAT CGATCAAACCAGAGGATGGTTTTACACCCTGCTGGTGCTAGCCACAGCGCTCTTTGGACAGCCACCTTTC AAGAATGTGATTGTGAATGGCCTCATCTTGGCAAGTGATGGCCAAAAAATGAGCAAGCGGAAAAAGAACT ACCCAGATCCAGTTTCCATCATCGATAAGTATGGTGCGGACGCCCTCAGGTTATATCTGATCAATTCCCC TGTGGTAAGAGCAGAAAACCTGCGCTTCAAGGAGGAAGGTGTGCGGGATGTCCTCAAAGATGTGCTGCTC CCGTGGTACAACGCTTACCGCTTCTTCATCCAGAACGTCTTTAGGCTCCACAAGGAAGAAGAAGTGAAGT TCCTATACAACGAGCATACAGTCAGAGAAAGCCCTAACATCACCGACCGCTGGGTTCTGTCCTTCATGCA GTCGCTCCTTGGCTTCTTTGAGACCGAAATGGCAGCTTACAGGCTGTACACTGTGGTACCTCGCCTGGTC AAGTTTGTAGATATTCTAACCAACTGGTATGTCCGAATGAACAGGAGGAGGTTAAAGGGTGAGAGTGGGG TGGAAGACTGTGTCATGGCTCTGGAGACACTGTTCAGTGTCCTGCTTTCTCTGTGCAGACTGATGGCCCC TTACACGCCCTTTCTGACTGAGCTGATGTACCAGAATCTAAAGCTGCTGATTGATCCTGCTTCTCTCCGG GACAAGGACACACTCAGCATCCACTACCTCATGCTGCCTCGAGTTCGAGAGGAGCTGATTGACAAGAAAA CTGAGAACGCCGTGTCTAGGATGCAGTCTGTGATCGAGCTTGGAAGAGTGATACGAGACCGAAAAACTAT TCCAATAAAGTATCCTCTGAAGGAAATTGTGGTCATCCACCAAGACCCAGAAGCTCTAGAGGATATCAGG TCCTTGGAGAAGTATATCATTGAGGAATTAAATGTTCGAAAAGTCACGCTGTCTACTGATAAAAACAAGT ACGGTATCCGACTAAGGGCTGAACCAGATCACATGGTCCTTGGGAAGCGCCTGAAGGGGGCCTTCAAAGC GGTGATGATGGCCATAAAGCGGCTGAGCAATGAGGAGCTGGAGCGGTTTCAGAAGAGCGGGAGCATTGTT GTGGAAGGCCACGAACTGCATGAGGAAGACATTCGCCTCATGTACACCTTTGACCAGGCCACGGGAGGGA CCGCGCAGTTTGAAGCACACTCTGATGCTCAGGCTCTAGTTCTCTTAGATGTCACACCTGACCAGTCAAT GGTGGATGAGGGCATGGCTCGGGAAGTCATCAATCGCATCCAGAAGCTTCGAAAGAAGTGCAATCTGGTT CCAACGGATGAAATAACAGTTTACTATAATGCAAAATCTGAAGGAAGATACTTGAATAATGTCATTGAAA GCCACACTGACTTCATATTTGCCACAATCAAAGCTCCCTTGAAGCCATATCCAGTTCCAACATCAGATAA TATCCTTATTCAAGAGCAAACACAGTTAAAGGGGTCCGAGCTGGAAATCACGCTCACCAAAGGATCGTGT GTGCCTGGCCCTGCTTGTGCTTATGTGAACCTTAACATTTGTGCAAATGGCACCGAGCAAGGTGGAGTGT TGCTCCTGGAGAATCCGAAAGGTGATAATCAGCTGAACCTGGTGAAACTGAAGACTGTTGTTACCAGCGT ATTTGGTGTTAAAAATGCAAAGCTGTCAGTCTTCCACGGTGAAACAGAAATACAGAACCAAACTGACTTG CTGAGCCTCAGTGGGAGGACGCTGTGTGTGACTGCAGGGGCATCCCCGTCTCCCATCAGCAGCCCCAGCA CCCTGCTGTGTCAGTACCTCAACCTGCAGCTCCTGAACGCAGAGCCACAAGAGTGTTTAACAGGAACTGT GGGCACCCTCCTGCTTGAAAACCCACTCGGACAGAACGGCCTCACCCACCAAGGGCTTGTGCATGAAGCA GCCAAGGTGTTCGGACTGAGGAGCAGGAGGCTAAGGCTGTTCCTGAATGAGACTCAAACACAAGAAATTA CAGAAGATATCCCAATGAAGACTTTGAATATGAAGACTGTATATGTTTCTGTCTTGCCAACAACTGCAGA TGGCTAACAGCTATCAGTATGCCTTCAGTCAGTCTTCCTTGAATCTACCCATCTCCTTATACACACACTC ACACACTCACACTCTCTATGTCTCTCTCTCTCTCTCTCTCTCTCTGTCTCTCTCTCACACACACATACAC ACACACACATGGGTTCAGTGAAGTTATCTCCTTGGGCACTTGTCAAATATGCTGCTTTTAACCGGCGATT CTAATGGGATTGACTGACTGGAGACCTCACATCATGTTCACATTTAACCTTAAACACCTATGCAGTCATG TTGGGGGAGTGTCCCAATGTTACCTCAACACCACGAAGCTTATTTCTATACTGGAGTTCTTCAGTATAGA AAGTGGAAGTGGTTTAAATGGCATAGTACATATACATATATATATGGCCTTGTAAAACAGATTTGAGACC TTGTGAAGAAATGGATATGTTATATATTTCTGCTACCTGGATTTTCCTGGGTAATTTGATGGAATATTTT ACGTTTGATTAAATCAAAACAGTAAACACTCTCTTAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: AscI-NotI
• ACCN: NM_172015
• Insert Size: 3789 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC067029, AAH67029
• RefSeq Size: 4398 bp
• RefSeq ORF: 3789 bp
• Locus ID: 105148
• UniProt ID: Q8BU30
• Cytogenetics: 13 A5
• Gene Summary: Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA.[UniProtKB/Swiss-Prot Function]