Zcchc3 (NM_175126) Mouse Untagged Clone

CAT#: MC205171

Zcchc3 (untagged) - Mouse zinc finger, CCHC domain containing 3 (Zcchc3), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Zcchc3
• Synonyms: 2810406K24Rik
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC055755
  CGAGAGCGGTAGGGCGGGCGCTCGTCCGCAGAGGGGAGGCGAGCCGGGGTCAAGACGAGCCGTCTCCGGC CGGCACGAATTCCCGCACGCAAGACTTCCAAGAGGGCGGAAGCCAGAAGCGTCGGGTTTCAGAGCGGGGG CGGGGAGTCGCCCACGCAGCCCGCGGCCCTCATCCCTCATCCCGAGCGGGAGCGCGCCATGGGCACCTCG GACCGCGGGAACGAGTGGAGGATGCTGGGAAAGAGGTAACATGGCCACCGGCGGCGGAGCGGAGGAGGAG TCACAGCGGGGCCGGCCTCAGCTCCCGCTCCCCGCGCGACCCGTGGCCCGCGTCGAGGAACCCGAGGGCG TCCGCGAGAAGATGGGCTGGGCCCAGGTGGTCAAGAACCTGGCGGAGAAGAAGGGCGACTTCCGCGAGCC GCGGCGGCGCGACGAAACTGGCAGCGGTGCAAGTGGCGGGCTCGGCAGCCCCGGGGGCCTGGCCACCCCG AACCCCGGCGACTTCCCACCCGCCGCTCGCGGGGACCCAAAGGGCCGCCGCAGAGACCCTACGGGTGAGG CGTCAGACGCTTACAGGAAAAAGAGCGCGAGCGGCGCGGGAGACCCGAGTCGAAGGAAGAAGGCCGAGGT GACGGCGGCCATGGCGACCCCCGCCAGGCCCGGCACGACCGAAGATGCCACCGAGCGACCGCTCCAGGAC GAGCCACCGGCTGCCGGCCCGGGTAAGGGCCGCTTCCTCGTGCGCATCTGTTTCCAGGGAGACGAAAGTG CCTGTCCGACCCGGGACTTCGTGGTGGGCGCGCTCATCCTGCGCTCCATCGGCATGGACCCTGACGACAT CTACGCGGTCATTCAGATCCCCGGCAGCCGCGAGTTCGACGTGAGTTTCCGATCCGCCGAAAAGCTAGCC CTGTTTCTCCGCGTCTACGAGGAGAAGCGAGAGCTGGAGGACTGCTGGGAGAACTTTGTGGTTTTGGGGC GGAGCAGGTCCAGCCTGAAGACCCTCTTCATCCTCTTCCGGAACGAGACCGTGGACGTGGAGGACATCGT GACCTGGCTCAAGCGCCACTGCGACGTGCTGGCCGTGCCCGTGAAAGTGACCGACAGGTTCGGGATCTGG ACCGGGGAGTACAAGTGCGAGATCGAGCTGCGCCAGGGGGAGGGCGGAGTGAGGCACCTGCCCGGGGCCT TCTTCTTGGGAGCGGAGAGGGGCTACAGCTGGTACAAGGGGCAGCCCAAGACGTGCTTTAAGTGTGGTTC CCGGACCCACATGAGCGGCACCTGCACGCAGGACAGGTGTTTCAGGTGCGGGGAAGAGGGGCACCTGAGC CCTTACTGCCGGAAGGTCATCGTGTGCAACCTCTGTGGCAAAAGAGGACACGCCTTTGCCCAGTGTCCCA AAGCAGTTCACAATTCCGTGACAGCTCAGCTCACCAGCGTGGCGGGGCACTGAAGGCGCGACTGCCTGCC AGGGTGAATACACTGCCAGCTTACCCCTCCCTTAAGTGCCAAAACTTTTTTTTTAACCTTTTTTTTTTAA TCGTTTTGAATGGAGATATTTCTAAAACCTACCAGAGACGTTCTCTCTATGCCTTTTTTAAACCGCGTGT GCTCCTTTTTGGCATGTTTGAAATGGTGAATTTTTGCCCGTAAGAACTATTCAGAACCATTGCATACAGA TATATTTTTTTTCTCCCGGTTTGTATTTGTTTTTGGTACTTTCGTCTACTCCCCTCCCCACTCTACTTTT TATTTTACTACCTCCCTCCTCATACATTTAATCCTTAAAAGGTTTTAGCCCTTTGGGCCGCCTTGGTCAT TATGCCTCCAGCCCAGGGTACTGGGCCCAACGCGGGATAGACACTCTTATCAAGTCTTTCATTGAATCAA AACGGTATCGACTGGCTTCTGTCAAAAGTGTCTACCTTTTGCAACTCTCTGCTGGGCCCTTTTTATTTGC TGCTTCTCTGTGGTATCAGACTTTGTGAAAACAGTGTTGCTAGAAGTGTGTATTCTGAATAAGCTCTGAA TGGTGGCCAAGGGCCTCTCGTGGCAAAAAAAAAAAAAAAAAA
• Restriction Sites: AscI-NotI
• ACCN: NM_175126
• Insert Size: 1203 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC055755, AAH55755
• RefSeq Size: 2072 bp
• RefSeq ORF: 1203 bp
• Locus ID: 67917
• UniProt ID: Q8BPK2
• Cytogenetics: 2 G3
• Gene Summary: Nucleic acid-binding protein involved in innate immune response to DNA and RNA viruses (PubMed:30193849, PubMed:30135424). Binds DNA and RNA in the cytoplasm and acts by promoting recognition of viral nucleic acids by virus sensors, such as DDX58/RIG-I, IFIH1/MDA5 and CGAS (PubMed:30193849, PubMed:30135424). Acts as a co-sensor for recognition of double-stranded DNA (dsDNA) by cGAS in the cytoplasm, thereby playing a role in innate immune response to cytosolic dsDNA and DNA virus (By similarity). Binds dsDNA and probably acts by promoting sensing of dsDNA by CGAS, leading to enhance CGAS oligomerization and activation (By similarity). Promotes sensing of viral RNA by RIG-I-like receptors proteins DDX58/RIG-I and IFIH1/MDA5 via two mechanisms: binds double-stranded RNA (dsRNA), enhancing the binding of DDX58/RIG-I and IFIH1/MDA5 to dsRNA and promotes 'Lys-63'-linked ubiquitination and subsequent activation of DDX58/RIG-I and IFIH1/MDA5 (By similarity).[UniProtKB/Swiss-Prot Function]