Bfsp1 (NM_009751) Mouse Untagged Clone

CAT#: MC205158

Bfsp1 (untagged) - Mouse beaded filament structural protein 1, in lens-CP94 (Bfsp1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Bfsp1
• Synonyms: CP95
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC057580
  GATCCTCCGTGCAGTCACATAGCAACAGGCCTCCACAATGGCGGAACAAAGAGGTCCTTGCCCGATGCCC CTTCCACCCGGGAGCTGTATGGGCGGCTTAATCCCGCACACAAAGCACCCGCGGCTCTTCATTGTTGGGC GCCCGCCCCGAGGGCACATCTGCTGACCGGCTCTGGAACGTCCCAGCCCGGCCGGCCGGTTTATTTAGCA GGCGCAGCCCAGGCCGCGGCCTTCCGGGAGCCATGTACCGCCGCAGCTACGTCTTCCAGGCCCGCCAGGA GCGCTACGAGCGCGCCCAGCCCGCGGGCCCCGCCGCCCAGCCCGGGGGAACCGCGCCCGGCCTGGCAGCG CTGCAGGCGCTGGGCGAGCGCGTGGCAGTCCAGGTCCAGCGGGCCCGCGCGCTCCAGCAGCGCCACGCCG GCCTGCGGCGGCAGCTCGACGCCTTCCAGCGCCTGGGCGAGCAGCCCGGGCCCGAGGACGCGCTCGCCCG CCACGTCGAGGCCAATCTGCAGCGCGCCCGGGACCTGACAGCCGAGCACGCGCGGCTCGAGAGGCAGGAG GCCGAGGCGCAGCGCGCGCTCGACGAGTTCCGCAGCAAATACGAAAATGAGTGTGAATGTCAGTTGGTGC TAAAGGAAATGCTGGAACGGCTTAACAAGGAAGCCGATGAAGCCTTATTGCGTAACCTGCACCTTCAGTT GGAGGCACAGTTTCTGCAGGCAGATATCAGTGTGGCCAAGGACAGGTACAAGAAGAATCTTCTGGAGATT CAGACCTACATCACCGTCCTGCAGCAGATCGTACAGACTGCTCCTCAAGTGTCCCTGGTTACTGGGATGA GGGAGGAGAAGCTGTTCACAGAACGGGAAGTGGCTGCCCTGCAGAACCAGCTGGAGGAGGGCCGAGAGGC AGTCACCCACCTGCAGGCACAGAAAGCTGAGCTGCAGGCCCAGACCACGGCCCTGGAACAAGCTATTAAG CATGCTCATGAGTGTTACGATGAGGAGCTACAGCTTTACAACGAGCAGATCGAAAACCTTCGGAAGGAGA TCGAGGAGGCGGAGCGGTCTCTGGAGAGGTCGTCCTATGACTGCAGGCAGCTAGCCGTGGCCCAGCAGAC GCTGAGGAATGAGCTAGACCGTTATCATCGGATCATTGAGATTGAAGGCAGCAGGCTGAGCTCTGTTTTC ATTGAGACGCCCATCTCTCTGATCACCCCGAGCCATGGGGCCCCTCTCAGCCTTGGATCCAGTGTGAAAG ATCTTGCCAGGGCCGTGCAGGATATTACAGCAGCTAAACCGAGACAGAAGGCCCTCCCCAAGAGCCTTCC AAAAAGAAAGGAGATTATAGCTCAAGATAAGGTGGAGGAAACTCTGGAAGATGCACCATTAAAACCTCCG CAGGAGCCAAAGGCACTGCAGGTGGAACGGAAAGCAGAAGGTGGCTCTCAGCCCGGAGCAGGGGGAGGAC ATGGAGTAAGTCCCACACAAGAAGGGGGTCCTGAGGATGTGCCAGACGGGGGCCAGATAAGCAAAGCCTT TGGGAAACTGTGCAAAGTAGTGAAAGAGAGAGTGAGCGGCCACAAAGAGCCCGAGCCTGAGCCGCCCACG GACCTTTTCACCAAAGGACGGCATGTACTCGTCACAGGGGAGTCTAGTTTTGTTGACCCCGAGTTCTACT CCTCCTCCATCCCAGCTAGAGGTGGGGTGGTGATTTCCATTGAGGAAGACTCTATGCATCATGACGGCCA CGTAGAGCCCTCTCCTGGACAGCCCATGCCACCTGTGGAGAATGGACAGGGCGTTCCCCAGGGCAGGGAA GGTGACCACTCAAACCATCAGCAGGGTACAGACAAGAATGGGTTACGGGCCAAGGAACCAAAAGACCTGG AGGAAAAAGATGATGATGGCAAGAAGGAAGCTGAGGGGAGCAGGAGACCCTGTCCTGTGATCATACCTGG TCCTGATGAACCATCTACATCCCATTCACAGACGTCTGGGTCCAATCAGGGTGGACCAGTGGGGCCTGCG AGTAAGAGCAGTAGCCTGCTGGCAAAGGGCCCTTCGAAGGCTCTGTCTATTAAGAAGGTGGAGGTGGTGG AATCCATTGAGAAGATTTCAACAGAGAGTATTCAGACATATGAAGAGACTTCCGTAATTGTGGAGACTCT GATTGGGAAGTCAAAGGGCAACAAGAAACTGGGAGAGAAGAGCTTGCCAGACACAAGGGCTTGAAGGAGG AAAGTGTCCGGGCAGTGTGGGAGGAGGGCTTTGAGATGGGCTCCTGTTTGGATTAGATCATAGTCAACCT GTCTGCATTGCAGGTGACCTCCAATGACCTTCATTAAACTGCTTTCTTTTTTGCTTGCACCAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: AscI-NotI
• ACCN: NM_009751
• Insert Size: 1992 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC057580, AAH57580
• RefSeq Size: 2414 bp
• RefSeq ORF: 1992 bp
• Locus ID: 12075
• UniProt ID: A2AMT1
• Cytogenetics: 2 G1
• Gene Summary: Required for the correct formation of lens intermediate filaments as part of a complex composed of BFSP1, BFSP2 and CRYAA (By similarity). Involved in altering the calcium regulation of MIP water permeability (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) uses an alternate in-frame splice site in the central coding region, compared to variant 1, resulting in an isoform (b) that is shorter than isoform a.