Itpk1 (NM_172584) Mouse Untagged Clone

CAT#: MC205100

Itpk1 (untagged) - Mouse inositol 1,3,4-triphosphate 5/6 kinase (Itpk1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Itpk1
• Synonyms: BC031182
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC056464
  CCGGACCCAGGCTCCCGGGCCGAGGAGGAAGAAGATGCAGACCTTCCTGAAAGGGAAGAGAGTTGGCTAC TGGCTGAGCGAGAAGAAAGTCAAGAAGCTCAATTTCCAGGCCTTCGCGGAGCTGTGCAGGAAGCGAGGGA TAGAAGTCGTGCAGCTGAACCTCAGCAGACCGATTGAGGAGCAGGGCCCCCTGGACGTCATCATCCACAA GCTGACCGATGTCATCCTCGAGGCAGACCAGAATGACAGCCAGTCCCTGGAATTGGTGCACAGGTTCCAA GAGTACATCGATGCTCACCCTGAGACCATCGTCTTGGATCCCCTCCCCGCCATCAGGACCCTGTTAGACC GTTCCAAGTCCTACGAACTTATCCGAAAGATCGAGGCCTACATGAAAGATGACAGAATCTGCTCGCCGCC TTTCATGGAGCTCACGAGCCTGTGTGGGGAGGACACCATGAGGCTGCTGGAGCAGAACGGCCTGGCCTTC CCCTTCATTTGCAAAACCAGAGTGGCTCATGGAACCAACTCTCATGAGATGGCTATTGTGTTCAACCAAG AGGGCCTGAATGCCATCCAGCCTCCCTGTGTGGTCCAGAACTTCATCAACCACAATGCTGTCCTGTACAA GGTGTTTGTGGTGGGCGAGTCCTACACAGTGGTCCAGAGACCCTCACTCAAGAACTTCTCTGCGGGCACA TCAGATCGTGAGTCCATCTTCTTCAACAGCCACAATGTGTCAAAGCCGGAGTCTTCATCAGTCCTCACTG AGCTGGACAAGATCGAGGGTGTGTTCGAACGGCCAAGCGATGAGGTTATCCGGGAGCTGTCCCGGGCTCT GCGGCAGGCGCTGGGAGTGTCACTGTTTGGAATTGACATCATCATTAACAACCAGACCGGGCAGCATGCA GTCATCGATGTCAATGCCTTCCCAGGCTATGAAGGAGTGAGTGAGTTCTTTACCGACCTCCTGAACCACA TTGCCACAGTTCTGCAAGGCCAGAGCACGGGAGGAGCTGCCACGGAGGAAGTGGCCCCGCTAAGGCACAA CAGGCTCCTGGCGGAACCGGCAGGCAGCCTGGCTGGGGAGCGGACGTGCAGTGCCAGCCCTGGCTGCTGT GGCAGCATGAAGGGCCAGGACACACCCTGGAAGACTGAGACCGAAGCAGGCAACATGGGCGCTGGTGCTT CCGCCAAGCTGCCGCACCAGAGACTTGGCTGCACCACTGGCGTATCACCCAGCTTCCAGCAGCACTGTGT GGCCTCTCTGGCCACCAAGGCTTCCTCACAGTAGCCACAGAGCCCAGGACCCAGAGGGGCAGAGCGTGGA AAATGCCCACTGGGCCAGCAGCTCCACATGGGAACACTACTAAGAATTCCTGGTGATCTGAGGCTTCTCT GTTTTAATTTTTTACCTGATTTTCTGATGTTATGATCGGAATGAAGGGGGGGAGACAGAGCAGAACATCA AGTGGCCCAGCCTCGAGGACGCCGCCTGACTCCTGCTGTGCAGAGGTCCTCACTGAGTTTACACACGCTT GTGTTCTCAACACTAGGTCTGAATGTGAGGGGGGGGGGTGCGTGTGTGTGTGTGTGTGTGTGTGTGTGTG TGTGTGTGTGTTTGTCATGGCAAGGGTGAGTGTGCATGTCTGTCTTCATGGCTGTTGTGGGCCAGGCTCT GGGCACCGAGGAAGGAAGCCCATATCTGGCCAGGCTGTTGCAGCTAAGCAGGCGAAGGACGAACGGTTTT CCCAGTTCCCCTCCTCTTCACACCTGCCCACACCCAGACCTCACCCTCCCTCTTGTCCCAGATGTGCTCT CCTCCAAGGCTAACCCTTGCCCCCCCACCTCGCTCTCCAAAGCCAGGGAAGCAGTAGACGCCCCCTCGGG AGCTCTGCATGGAGGATCTGGGTTGTAGGAAGGGAAAGGAGCTTCCTGTGGGTGTAGCCGAAGCCCACTG GCTATTGCTAGTCTTCACTGGCTCGTGGAATACAGCAGGCCACTATCCACCCTACCCTGACCCTATTGCT CACTGAGATCCAGGAAGGAGCCATCTGACTAGGAGTCCAGGAGTCTGCCAGGACCCAGGATTTACCCTGG GACTTCCAACAGATCATTCCGTGAGTCTTCAGGCCTCCACCACCTCATCTCTTCAGGGTGGCTCCTGAAA CAGAGAACTGAAGGCCTGGTCTAGTGGTCTAGCCCACGAGGATCTCTGCTTCCTCATGTCATCATCCGGT CCCTCTTTTCAGGTGAGAGGAGACCACCCACAGGGCTGCTCCTGTTCACACGCGACAGCCCCTCCTGGAA CAGCAAGGCCCAGCTCCTTCCTGCTGTGTTCATAGCGAGTCCCTTGCACTGAGCACCAGGCAAGCCCCCA AGGGAGTTGTGCCCCATTTCAGACTCACTCTGACCCCACAGCAGCTGGCAGACCACCCACTGGCCCCAAA GCCCACAGGAAAGGACCCCTGTGGAGCCCCAGGTCAGCAGCCCAGGACCCCCAAGGGTGATCTTGTGTCT TAAGACATCAGCTCACTATGTAAAGAGAATGAGGCCTCTGGGTTGTCCTTAGGTCTAATAAGATAGAGTA CACTAGAAGCAGCAGCCCTGGGAAGGCCACTAAGGGCCATGCAGGGGCATCCTTTGGGCAAGGAGAAGCC GATTGGCTTCCTCGGAAGCAGAGCCCCAGGACTAACCACGTTTACAGGACCTGAGTGTTGAACTGATTGA TGTCTGTAAGTCACCTTCCCAGGCCTGACCCTGAAAACAGGGGGTGGGAAGCGTGGCCGCCTCTTGCACT GCTTTGTCTCCGGAATAAACTACTGAGATCAAAAAAAAAAAAAAAAAAA
• Restriction Sites: AscI-NotI
• ACCN: NM_172584
• Insert Size: 1260 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC056464, AAH56464
• RefSeq Size: 2849 bp
• RefSeq ORF: 1260 bp
• Locus ID: 217837
• UniProt ID: Q8BYN3
• Cytogenetics: 12 E
• Gene Summary: Kinase that can phosphorylate various inositol polyphosphate such as Ins(3,4,5,6)P4 or Ins(1,3,4)P3. Phosphorylates Ins(3,4,5,6)P4 at position 1 to form Ins(1,3,4,5,6)P5. This reaction is thought to have regulatory importance, since Ins(3,4,5,6)P4 is an inhibitor of plasma membrane Ca(2+)-activated Cl(-) channels, while Ins(1,3,4,5,6)P5 is not. Also phosphorylates Ins(1,3,4)P3 on O-5 and O-6 to form Ins(1,3,4,6)P4, an essential molecule in the hexakisphosphate (InsP6) pathway. Also acts as an inositol polyphosphate phosphatase that dephosphorylate Ins(1,3,4,5)P4 and Ins(1,3,4,6)P4 to Ins(1,3,4)P3, and Ins(1,3,4,5,6)P5 to Ins(3,4,5,6)P4. May also act as an isomerase that interconverts the inositol tetrakisphosphate isomers Ins(1,3,4,5)P4 and Ins(1,3,4,6)P4 in the presence of ADP and magnesium. Probably acts as the rate-limiting enzyme of the InsP6 pathway. Modifies TNF-alpha-induced apoptosis by interfering with the activation of TNFRSF1A-associated death domain (By similarity). Plays an important role in MLKL-mediated necroptosis. Produces highly phosphorylated inositol phosphates such as inositolhexakisphosphate (InsP6) which bind to MLKL mediating the release of an N-terminal auto-inhibitory region leading to its activation. Essential for activated phospho-MLKL to oligomerize and localize to the cell membrane during necroptosis (By similarity).[UniProtKB/Swiss-Prot Function]