Dnaaf1 (NM_026648) Mouse Untagged Clone

CAT#: MC204904

Dnaaf1 (untagged) - Mouse leucine rich repeat containing 50 (Lrrc50), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Dnaaf1
• Synonyms: 4930457P18Rik; Lrrc50; m4Bei
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC050751
  GCGGACCGTAGAGTATTAGGGCTGAGTGGTCGGGCCAGGAGTGAGCATGCACCCCGAAGTCTCTGAGCCG CCGGTGGACAGCGTGGCGGAGCCCAGTCTGGAGGAGTCGGCAGGCGATCACGGCGACGCGGGGCCAGGGA TCCGTAAAGAAGAAATTAGTGAGACCAAGGAAACATGTGCGGGTCCTTGCACAACATCCTGCCCAAGTCA GCAGCAACCAAGTGGCGACAACGGGTCAGAGGGCTTCTGCACCCACTCGAGAGATGACAGAGAGGATCGG GGCCCCAGGATGACGAAACAGTTCCTTCAAAAGCTCTGCAAGCAGCACAAGCTGTACGTCACCCCCGCCC TGAACGACACCCTGTATTTGCACTTTAAGGGCTTTGACCGCATTGAGAACCTGGAGGAGTACACGGGCCT GCGCTGCCTCTGGCTGGAGTGCAATGGCATCCAGAGGATCGAGAATCTGCAGGCCCAGAGCGAACTGCGC TGCCTCTTCCTGCAAGTGAACCTGTTGCACAAGATCGAGAACCTGGAGCCCCTCCAGAAGCTGGACGCCC TCAACCTGAGCAACAACTACATCAAGACCATTGAGAACCTCTCCTGCCTCCCTGTCCTGAACACACTGCA AATGGCCCACAACCGCCTGGAGACGGTGGCTGACATTGAGCACCTCAGGGAGTGTCTGCGACTCTGTGTC CTCGACCTCTCCCACAATGCGCTGAGTGACCCTGAGATCCTGAGCGTGCTAGAGAGCATGCCCTGTCTGC GTGTGCTGAACCTCATGGGAAACCCAGTGACCAAACACATCCCTAACTACCGCAGGACGGTCACTGTCCG TCTGAAGCACTTGACTTATCTGGATGACAGGCCAGTTTTCCCCAAGGACAGGGCGTGTGCAGAGGCATGG GCCCGAGGTGGGTATGCCGCCGAGAAGGAAGAGCGTCGCCAGTGGGAGAGCAGGGAACACAAGAAGATAA CAGACAGCCTGGAAGCCTTGGCCATGATCAAGCGTCGGGCTGAGGAGCGGAAGAAGGCAAGAGATAGAGG GGAGACACCCCTGCCAGACAGCGAAGGGAGTATCCCCACCAGCCCGGAGGCTGAGGAAAAGCAACCCATG GGGGAAATCCAGAAGAAGATGGAGTTGTTTGTGGAGGAGAGCTTTGAAGCCAAAGATGAACTGTTTCCAG AGACGCCAGGAGGAGAAAAGGAGCTGCATGTGGTTGTGGTGAATGGGGCAGTGGAGAATCCAGACCTGTC TGGGAGCCTGGCTCACAACCAGACACCTGTGGTGGTCACCCCGGAAGAGTCTACATCTCCAGTGGCAGCT ACTGATGGGGCCAGAACTGAAGATATAGAGGCCGTTGCCGTGGAGATCAAGGAGAGGCTTTTCATTGATG ACCTTCCTGACTTGGAAGATGCTGAGGGTACAGATGTGTCTGTGGAAGACCAGATAAAGGAGACAGACAT CCCGAGAATCCAGGCCATTTCCAGCTTGAGTGACGACAGTGACCCGGAGCTGGACGAGCTGTCCCTCTCC ACGTCTGAGGCCACTCCCACAGGCGCCACAGGTGCCCTCTCACACATATTTGCCATCTCCAAGGGCCCCT CCACGGCAGCCACGGTGCCTTTCACAGACATATGTAAGCCAATAGCAACAACGGACTTAGAAAGCCAAGG TCAAGACTGCGGTGCAGCGGCCTCACGCCCCCTCATCCAGGAGCTCAATGATGAGCCGGCAGAGGAAGCA GCAAACCAGCCACTGCCGCCCCAAACCTGTGCGAGTGACCCTGCACTTGCCCACCCTAGTGAAGATGGAG ACAGCGACAGCCAGCTGCCTGCAGCCACTCTGCTAGGAGATGGAGCGGAGGATGAAGCAGAGTCTTCGGT GCACCCGAAGGAGCCCAGCACCCGGGTAGGCCTAGAAGATATTGAATTTGGCTTGGACTGAACCCCAAGG CAGTCTACATTGTTGGGCTAGCGGTGGCGCAGCAGATGCAGGGCACCCTCTCCCGTGCATCACTGTGCTC TTGAGCCTGCAAGGCAGGTGCGTGAAGCTACACCAATGTGCCTTGTACCCAACACGTCGGTATCCCCTGG TTACAGACCCCACAAGAACATAAACACTTTAAGACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: SfiI-SfiI
• ACCN: NM_026648
• Insert Size: 1905 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC050751, AAH50751
• RefSeq Size: 2167 bp
• RefSeq ORF: 1905 bp
• Locus ID: 68270
• UniProt ID: Q9D2H9
• Cytogenetics: 8 E1
• Gene Summary: Cilium-specific protein required for the stability of the ciliary architecture. Plays a role in cytoplasmic preassembly of dynein arms (By similarity). Involved in regulation of microtubule-based cilia and actin-based brush border microvilli (By similarity).[UniProtKB/Swiss-Prot Function]