Apex2 (NM_029943) Mouse Untagged Clone

CAT#: MC204878

Apex2 (untagged) - Mouse apurinic/apyrimidinic endonuclease 2 (Apex2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Apex2
• Synonyms: ape2; C430040P13Rik
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC078633
  CTTCCTTTGGGAAACTGTACCGTTTACCAAAATGCTGCGCGTGGTAAGCTGGAACATCAATGGGATCCGG AGTCCCCTGCAAGGCCTTGCATGCCAGGAACCCAGCAGCTGTCCCACGGCCTTGCGACGCGTTTTGGACG AGCTGGATGCTGATATTGTCTGTCTCCAGGAGACCAAAGTGACCAGAGATGTACTGACAGAGCCCCTGGC TATTGTTGAGGGTTATAACTCCTATTTCAGCTTCAGCCGCAGCCGTAGTGGCTATTCTGGTGTGGCTACC TTCTGTAAGGACAGTGCTACCCCAGTAGCTGCTGAAGAAGGCCTGAGTGGTGTGTTTGCCACCCTGAATG GAGATATTGGTTGCTATGGAAACATGGATGAGTTCACGCAAGAGGAACTCCGGGTTCTGGATAGTGAGGG CCGGGCCCTCCTTACACAGCACAAGATCCGCACATTGGAAGGGAAGGAGAAGACTTTGACCCTGATCAAT GTGTACTGCCCTCACGCGGATCCTGGGAAGCCTGAGCGGCTGACCTTCAAGATGCGCTTCTATCGCCTGC TCCAGATGCGAGCAGAAGCGCTCCTGGCAGCTGGCAGTCACGTGATAATCCTGGGGGACCTGAATACAGC CCACCGACCCATTGACCACTGCGATGCAAGTAGTCTGGAATGCTTTGAAGAGGACCCAGGGCGTAAGTGG ATGGATGGCTTGCTCAGTAACCCAGGGGATGAGGCTGGACCCCACATAGGGCTCTTCATGGATAGCTACC GCTACTTGCATCCAAAACAGCAGAGGGCTTTCACCTGCTGGTCAGTGGTCAGTGGTGCACGCCATCTCAA CTATGGCTCTAGACTTGACTATGTACTGGGAGATAGGGCCTTGGTCATAGATACCTTCCAGGCCTCCTTC TTACTCCCTGAAGTGATGGGCTCTGACCACTGCCCTGTGGGAGCTGTCTTAAATGTATCCTGTGTGCCAG CAAAACAGTGCCCAGCTCTGTGTACCCGCTTCCTCCCCGAGTTTGCAGGTACCCAGCTCAAGATTCTTCG CTTCCTAGTTCCTCTTGAGCAAGAACCTGTGCGGGAACAGCAAGTCCTGCAGCCCAGCCATCAAATCCAG GCACAGAGACAGCCAAGAAAAGCCTGCATGCATTCAACCAGGCTTCGGAAAAGTCAAGGTGGCCCCAAAA GAAAACAGAAAAACCTGATGAGTTACTTCCAGCCTTCCTCCAGCCTTTCCCAAACTTCTGGTGTGGAGCT GCCTACCCTGCCTCTGGTGGGCCCTCTTACAACCCCAAAGACTGCAGAAGAGGTGGCAACAGCCACAGTG CTAGAAGAGAAGAACAAGGTTCCAGAGTCCAAAGATGAGAAGGGAGAAAGGACTGCCTTCTGGAAGTCTA TGCTGAGTGGGCCCTCACCCATGCCCCTCTGTGGAGGCCACAGGGAGCCATGTGTGATGCGTACTGTGAA AAAAACAGGACCCAACTTTGGTCGCCAGTTCTACATGTGTGCTAGACCCCGAGGTCCTCCCAGTGACCCC TCTTCCCGCTGTAACTTCTTCCTCTGGAGCAGGCCCAGCTGAACAGACTGAGGCATAGGGATGTCTTCGT GTTCACCCCGTGCATGGGGTCAGATGCCAGGTCCCTGCCTATGAAGGCTTCTTTTTGTTCTCTGCTTTCC CTCCACTCCTTCCCTTCTCCCCCTAATGCCTCTCCCCTTTCACATCTCTGTTTCTTCCTCTTTGCACATG GAGGAGCAGGGAACTAATTTAATCCCCTTCAACCCTTGTTAGGGCATAAATAACATTTTGTTCATAAATA AAGGTCACGTCTTTGTTTCAGTAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_029943
• Insert Size: 1551 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC078633, AAH78633
• RefSeq Size: 1857 bp
• RefSeq ORF: 1551 bp
• Locus ID: 77622
• UniProt ID: Q68G58
• Cytogenetics: X F3
• Gene Summary: Function as a weak apurinic/apyrimidinic (AP) endodeoxyribonuclease in the DNA base excision repair (BER) pathway of DNA lesions induced by oxidative and alkylating agents. Initiates repair of AP sites in DNA by catalyzing hydrolytic incision of the phosphodiester backbone immediately adjacent to the damage, generating a single-strand break with 5'-deoxyribose phosphate and 3'-hydroxyl ends. Displays also double-stranded DNA 3'-5' exonuclease, 3'-phosphodiesterase activities. Shows robust 3'-5' exonuclease activity on 3'-recessed heteroduplex DNA and is able to remove mismatched nucleotides preferentially. Shows fairly strong 3'-phosphodiesterase activity involved in the removal of 3'-damaged termini formed in DNA by oxidative agents. In the nucleus functions in the PCNA-dependent BER pathway. Required for somatic hypermutation (SHM) and DNA cleavage step of class switch recombination (CSR) of immunoglobulin genes. Required for proper cell cycle progression during proliferation of peripheral lymphocytes.[UniProtKB/Swiss-Prot Function]