Pdgfrb (NM_008809) Mouse Untagged Clone

CAT#: MC204737

Pdgfrb (untagged) - Mouse platelet derived growth factor receptor, beta polypeptide (Pdgfrb), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Pdgfrb
• Synonyms: AI528809; CD140b; Pdgfr; PDGFR-1
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC055311
  AAGAGGTAATCCGGGGCAGAGAAAGCCCACAGTGGTGTGAGCTCTGGGGCTGTCTGTGGCCACAGTCCCG GCTACCCTATCTGGGACCTAGGATTGCTCTGGGAGTAACTTTGAGAGAGAGGAGAACAGAGAGGAAACAG TCCAGAGCCAGAGCGGGCCCAGACCAGTCGTCAGTCTCCTGCCTGCCAGCTAGACCTAGGGCGGCCCCTC GGGCTGCTCCGCTCCTCCCGGAGGATGCTTTTGGAGTGAGGAGGGGCCGGGCTGCTTCTCACCCCTGAGC ACCCTCTCCATTCCCCTGATTCTCTCAGGGTTTTCCGCAATCAGGCCAGCCCTTCTACTGCTGTCCGTTT TTTGGGTCCAGCAAAATAACAGAAGACAGCGAGGTGGACTTCCTGGAGGGGGTGATAGCTCACATCAGAA GCCATCTGTAGCCCGGACACCATGGGGCTTCCAGGAGTGATACCAGCTTTAGTCCTCAGAGGCCAGTTGT TGCTGTCCGTGTTATGGCTCCTGGGACCGCAGACCTCCCGGGGCCTAGTCATCACGCCCCCTGGGCCAGA GTTTGTTCTCAACATCTCCAGCACCTTTGTTCTGACCTGCTCGGGCTCAGCTCCGGTGATGTGGGAACAG ATGTCCCAGGTGCCCTGGCAAGAAGCGGCCATGAATCAGGACGGCACCTTCTCCAGTGTGCTGACACTGA CCAATGTCACTGGGGGAGACACTGGGGAATACTTTTGTGTCTACAACAACTCACTAGGGCCGGAGCTCAG TGAGAGGAAGCGTATCTATATCTTTGTGCCAGATCCCACCATGGACTTCCTCCCTATGGACTCCGAGGAC CTGTTCATTTTTGTCACGGATGTCACTGAGACGACAATTCCGTGCCGAGTGACAGACCCCCAGCTGGAGG TGACGCTACATGAGAAGAAAGTGGATATCCCCCTACACGTACCCTACGACCACCAGCGAGGTTTCACTGG TACTTTTGAGGACAAGACTTACATTTGCAAAACCACCATTGGGGACAGGGAAGTGGACTCCGATACTTAC TACGTCTACAGCCTCCAGGTGTCATCCATCAACGTCTCTGTGAATGCCGTGCAGACTGTGGTCCGCCAGG GCGAGAGCATCACCATCCGGTGCATTGTGATGGGCAATGATGTGGTGAACTTCCAATGGACGTACCCCCG CATGAAGAGTGGGCGGCTGGTGGAGCCAGTGACAGACTACCTCTTTGGAGTGCCCTCCCGCATTGGCTCC ATCCTGCATATCCCCACGGCTGAGCTGAGTGATTCGGGCACCTATACTTGCAACGTGTCAGTGAGTGTGA ACGACCATGGCGATGAGAAAGCCATCAACATCTCTGTGATCGAGAATGGCTACGTGCGGCTGCTGGAGAC ACTGGGAGATGTAGAAATTGCTGAGCTGCACCGGAGTCGGACGCTGCGGGTGGTGTTCGAGGCTTATCCG ATGCCTTCTGTCCTGTGGCTCAAGGACAACCGTACCTTGGGTGACTCCGGCGCTGGCGAGTTAGTTTTGT CTACTCGCAACATGTCTGAGACCCGGTACGTGTCAGAACTGATCCTGGTACGTGTGAAGGTGTCAGAAGC GGGCTACTATACTATGCGAGCCTTCCACGAGGACGATGAGGTCCAGCTCTCCTTCAAGCTGCAGGTCAAT GTCCCCGTCCGTGTGCTGGAGCTGAGTGAGAGTCACCCTGCCAATGGGGAGCAGACAATCCGCTGTCGTG GCCGGGGCATGCCTCAGCCAAATGTCACCTGGTCTACCTGCAGAGACCTCAAAAGTAGGTGTCCACGAAA ACTGTCACCCACACCCTTGGGGAATAGTTCCAAGGAGGAGAGCCAGCTAGAAACGAATGTGACTTTCTGG GAGGAAGATCAGGAATACGAGGTGGTGAGCACACTGCGCCTGCGCCACGTGGATCAGCCACTGTCCGTAC GCTGCATGCTGCAGAACTCCATGGGTGGAGATTCGCAGGAGGTCACCGTGGTCCCACATTCCTTGCCCTT CAAAGTGGTGGTGATCTCAGCCATCCTGGCCTTAGTGGTCCTTACCGTCATCTCTCTCATCATCCTCATC ATGCTGTGGCAGAAGAAGCCACGCTATGAGATCCGATGGAAGGTCATTGAGTCTGTGAGCTCTGACGGTC ATGAGTACATCTACGTGGACCCTGTGCAGTTGCCTTACGACTCCACCTGGGAGCTGCCACGGGACCAGCT TGTTCTGGGACGCACTCTTGGCTCTGGGGCTTTCGGACAGGTGGTGGAGGCCACAGCTCACGGTCTGAGC CATTCGCAGGCCACCATGAAAGTGGCTGTCAAGATGCTGAAATCGACAGCCAGAAGTAGCGAGAAGCAAG CCTTAATGTCCGAGCTGAAGATTATGAGTCATCTTGGACCCCACCTGAACGTGGTCAACCTGCTGGGGGC CTGCACCAAAGGAGGGCCCATCTACATCATCACGGAATACTGCCGATACGGTGATCTGGTGGACTACCTG CACCGGAACAAACACACCTTCTTGCAGCGACACTCCAACAAGCATTGTCCGCCCAGTGCTGAGCTCTACA GCAACGCCCTGCCAGTGGGGTTCTCCCTACCCAGCCACTTGAACCTGACTGGGGAGAGTGACGGTGGCTA CATGGATATGAGCAAGGATGAATCTATAGATTACGTGCCCATGTTGGACATGAAAGGAGACATCAAATAC GCAGACATTGAGTCCCCCAGCTACATGGCCCCTTATGATAACTATGTCCCATCTGCCCCTGAAAGGACCT ATCGCGCCACCTTAATCAACGACTCACCAGTGCTCAGCTACACAGACCTCGTGGGCTTCAGCTACCAAGT GGCCAACGGCATGGACTTCTTAGCCTCTAAGAACTGTGTTCACCGAGACTTGGCGGCCAGGAATGTGCTC ATCTGCGAGGGCAAGCTGGTCAAGATCTGTGACTTCGGCCTGGCTCGAGACATCATGAGGGACTCAAACT ACATCTCCAAAGGCAGCACCTTCCTGCCTCTGAAGTGGATGGCCCCAGAGAGCATCTTCAACAGCCTCTA CACCACTTTGAGTGATGTCTGGTCTTTTGGGATCCTACTCTGGGAGATCTTCACACTGGGTGGCACCCCT TACCCAGAGCTGCCCATGAACGACCAGTTCTACAATGCCATCAAGAGGGGCTACCGCATGGCCCAGCCTG CTCATGCCTCCGACGAGATCTATGAGATCATGCAGAAATGCTGGGAAGAAAAGTTTGAGACTCGACCCCC CTTCTCCCAGCTGGTGCTGCTCCTGGAGAGGCTTCTGGGTGAAGGCTATAAAAAGAAGTACCAGCAGGTA GATGAGGAGTTCCTGAGGAGTGACCATCCTGCCATCCTGAGGTCCCAAGCCCGCTTTCCGGGGATCCACA GCCTCCGATCCCCTCTGGACACCAGCTCTGTTCTCTACACTGCCGTGCAGCCCAATGAGAGTGACAATGA CTACATCATCCCCTTACCTGACCCCAAGCCTGACGTTGCTGATGAAGGTCTCCCAGAGGGGTCCCCCAGC CTTGCCAGTTCCACCTTGAATGAAGTCAACACTTCCTCCACCATCTCCTGCGACAGTCCCCTGGAGCTCC AAGAAGAGCCACAGCAAGCAGAGCCTGAGGCACAACTGGAGCAGCCACAGGATTCAGGCTGCCCAGGACC TCTGGCTGAAGCAGAGGACAGCTTCCTGTAGGAACTGACATCACTCCATTTTGCCCGAATCTCCCTTCTG CCTCCCAGAACCCAACCCTCTTGGCCTGGCCTGGCCTGGCCTGGCCTCCCAGCAGCTACACTGCCACAAG CTGTCCCCTCGAGGAAAGCCCTTGGTTTGCAGCACTCAAGGTCCAGGGACCCAGCTTAGCTTAGGAGGCA AGAGAACTCTGCCTCGGGAAGGTCATGGGACTCTGAACCAGGGTTCCTCCAGGGGACTCAGTTTCCCCAA ATGTAAGAGGAAGAGTTGTACTTGGCTACAGGACAGGTCTGGAGCCCGGATTCCTGCAGAAATCCACGAC TGTACGGTGGTGTGTTCATATCCTCCTGTGTAGCAGCTGCCCCTCAGCTGGATTGCTCTACTTTGATCTT CCTAAGAATCAGGCAAGGACCTGGTGTCTGGCTCCTGGCCAAACTGTAACCAGCCTTGGACAAGGTCTTT TCATTCAGAGCCCACCTCCCCTGGTCTTAGCTTTCCCAGGCCCGAGCTGGTCTGGGGCCAGCCATGCTTA ATGAATGCTGTTAGTGGTGAAGGTAGGCCGAGTACAGAATGCTCTGGCCTGCAGCCCTGCCTGGGCACTC AGGGCACACCTGGCCACAGGAAGCACCCACTCCTTTCAGCCCCACCAGTCCTAGAATAGTCCCCAGGTCA CTCTCAGCTGACCCACCCACCAGAGTCTGCAGGGCCATTGTCCACGCCTGTGATAGGCTAAGCATCTGCC TGAAGTGTGTACCTACCACTAGGAGCCCTGGCTCTGCGCTGGACCTGCTATGAGACCTTGGGGGCTTTCC TTGTTCTCTCTGGGGACCAGTTTTCTTTCCCCTTTGAAAAGCAAGTTGGACACATAGACTCTGAGTACCC TGTCAATAGTACAATTCTTGTATGTTCTGGGAGTTTGCTCTTGTCCCGAGGAAGCAGGGTCAAGTCCTTA AACTGATCTTTCTAGGGGTCAGCTGAGCTCTTGGAAGATCCCTGATACACTTCACACTCTGGGGGTTCAG GAACCGAGCCTCTTCTTCAAGTCTCCAAGTGCAACTGCCCAGACCTTGACTTGGAGTGACAGTGAGTGTC CTAGGAAACCCCCTTACAGCTGTCCTGAGGACACAGAAGAGACCACGAGACCCTTTTTTTCATATCATGA AGCCAGCAAGAGTGGCAGAGAAGGCAAGCCAGGTTACCTCGGGCCACTGTCACCAGCAGCGTGTGGACAG ACATGATGGACAGTGAGGACAGACGTCCCATGCAGGACAAGAGACTTGAGATGGGCAATGAGAGGAAGAT GCTGACGGGATTACCACCTCAACTGCCAGCTGCCCCCGTCCCCAGGGAGCACCCCACAGAAACAGTTCTA ACCCTGAACCAATGAACATTGCAAGTGCCTGGGGACTAGGGAGTGGGGGGAAGTCAGCCTTTCTGGGGAC CCTCCCTGCTAGCTGGTTGGCTAGCTGGCATCTCCCCGCTTGGAGCAGCAGAGGCTGGGGAGTACTGCTC ACAATGGTACCAAAGATAGAATCACCTAGGTTTACAAGTACTTGTAGGACTCGAGATAACCCACATTTAG ACACCGGAAGTGCTATTTTATATGCTGTTAAGTTTTCCTATCTGTACTTTTTTTTTAAATGGGAAAGATT TTAATATTAAACTTGGTGCTTCTCACTGAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_008809
• Insert Size: 3300 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC055311, AAH55311
• RefSeq Size: 5435 bp
• RefSeq ORF: 3300 bp
• Locus ID: 18596
• UniProt ID: P05622
• Cytogenetics: 18 34.41 cM
• Gene Summary: Tyrosine-protein kinase that acts as cell-surface receptor for homodimeric PDGFB and PDGFD and for heterodimers formed by PDGFA and PDGFB, and plays an essential role in the regulation of embryonic development, cell proliferation, survival, differentiation, chemotaxis and migration. Plays an essential role in blood vessel development by promoting proliferation, migration and recruitment of pericytes and smooth muscle cells to endothelial cells. Plays a role in the migration of vascular smooth muscle cells and the formation of neointima at vascular injury sites. Required for normal development of the cardiovascular system. Required for normal recruitment of pericytes (mesangial cells) in the kidney glomerulus, and for normal formation of a branched network of capillaries in kidney glomeruli. Promotes rearrangement of the actin cytoskeleton and the formation of membrane ruffles. Binding of its cognate ligands - homodimeric PDGFB, heterodimers formed by PDGFA and PDGFB or homodimeric PDGFD -leads to the activation of several signaling cascades; the response depends on the nature of the bound ligand and is modulated by the formation of heterodimers between PDGFRA and PDGFRB. Phosphorylates PLCG1, PIK3R1, PTPN11, RASA1/GAP, CBL, SHC1 and NCK1. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate, mobilization of cytosolic Ca(2+) and the activation of protein kinase C. Phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, leads to the activation of the AKT1 signaling pathway. Phosphorylation of SHC1, or of the C-terminus of PTPN11, creates a binding site for GRB2, resulting in the activation of HRAS, RAF1 and down-stream MAP kinases, including MAPK1/ERK2 and/or MAPK3/ERK1. Promotes phosphorylation and activation of SRC family kinases. Promotes phosphorylation of PDCD6IP/ALIX and STAM (By similarity). Receptor signaling is down-regulated by protein phosphatases that dephosphorylate the receptor and its down-stream effectors, and by rapid internalization of the activated receptor.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) contains an alternate in-frame splice site, compared to variant 1, that results in an isoform (2) that is one amino acid shorter than isoform 1.