Pdgfrb (NM_008809) Mouse Tagged ORF Clone

CAT#: MR225618

Pdgfrb (Myc-DDK-tagged) - Mouse platelet derived growth factor receptor, beta polypeptide (Pdgfrb), transcript variant 2

Product Images

Circular map for MR225618

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Pdgfrb
• Synonyms: AI528809; CD140b; Pdgfr; PDGFR-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR225618 representing NM_008809
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGCTTCCAGGAGTGATACCAGCTTTAGTCCTCAGAGGCCAGTTGTTGCTGTCCGTGTTATGGCTCC
  TGGGACCGCAGACCTCCCGGGGCCTAGTCATCACGCCCCCTGGGCCAGAGTTTGTTCTCAACATCTCCAG
  CACCTTTGTTCTGACCTGCTCGGGCTCAGCTCCGGTGATGTGGGAACAGATGTCCCAGGTGCCCTGGCAA
  GAAGCGGCCATGAATCAGGACGGCACCTTCTCCAGTGTGCTGACACTGACCAATGTCACTGGGGGAGACA
  CTGGGGAATACTTTTGTGTCTACAACAACTCACTAGGGCCGGAGCTCAGTGAGAGGAAGCGTATCTATAT
  CTTTGTGCCAGATCCCACCATGGGCTTCCTCCCTATGGACTCCGAGGACCTGTTCATTTTTGTCACGGAT
  GTCACTGAGACGACAATTCCGTGCCGAGTGACAGACCCCCAGCTGGAGGTGACGCTACATGAGAAGAAAG
  TGGATATCCCCCTACACGTACCCTACGACCACCAGCGAGGTTTCACTGGTACTTTTGAGGACAAGACTTA
  CATTTGCAAAACCACCATTGGGGACAGGGAAGTGGACTCCGATACTTACTACGTCTACAGCCTCCAGGTG
  TCATCCATCAACGTCTCTGTGAATGCCGTGCAGACTGTGGTCCGCCAGGGCGAGAGCATCACCATCCGGT
  GCATTGTGATGGGCAATGATGTGGTGAACTTCCAATGGACGTACCCCCGCATGAAGAGTGGGCGGCTGGT
  GGAGCCAGTGACAGACTACCTCTTTGGAGTGCCCTCCCGCATTGGCTCCATCCTGCATATCCCCACGGCT
  GAGCTGAGTGATTCGGGCACCTATACTTGCAACGTGTCAGTGAGTGTGAACGACCATGGCGATGAGAAAG
  CCATCAACATCTCTGTGATCGAGAATGGCTACGTGCGGCTGCTGGAGACACTGGGAGATGTAGAAATTGC
  TGAGCTGCACCGGAGTCGGACGCTGCGGGTGGTGTTCGAGGCTTATCCGATGCCTTCTGTCCTGTGGCTC
  AAGGACAACCGTACCTTGGGTGACTCCGGCGCTGGCGAGTTAGTTTTGTCTACTCGCAACATGTCTGAGA
  CCCGGTACGTGTCAGAACTGATCCTGGTACGTGTGAAGGTGTCAGAAGCGGGCTACTATACTATGCGAGC
  CTTCCACGAGGACGATGAGGTCCAGCTCTCCTTCAAGCTGCAGGTCAATGTCCCCGTCCGTGTGCTGGAG
  CTGAGTGAGAGTCACCCTGCCAATGGGGAGCAGACAATCCGCTGTCGTGGCCGGGGCATGCCTCAGCCAA
  ATGTCACCTGGTCTACCTGCAGAGACCTCAAAAGGTGTCCACGAAAACTGTCACCCACACCCTTGGGGAA
  TAGTTCCAAGGAGGAGAGCCAGCTAGAAACGAATGTGACTTTCTGGGAGGAAGATCAGGAATACGAGGTG
  GTGAGCACACTGCGCCTGCGCCACGTGGATCAGCCACTGTCCGTACGCTGCATGCTGCAGAACTCCATGG
  GTGGAGATTCGCAGGAGGTCACCGTGGTCCCACATTCCTTGCCCTTCAAAGTGGTGGTGATCTCAGCCAT
  CCTGGCCTTAGTGGTCCTTACCGTCATCTCTCTCATCATCCTCATCATGCTGTGGCAGAAGAAGCCACGC
  TATGAGATCCGATGGAAGGTCATTGAGTCTGTGAGCTCTGACGGTCATGAGTACATCTACGTGGACCCTG
  TGCAGTTGCCTTACGACTCCACCTGGGAGCTGCCACGGGACCAGCTTGTTCTGGGACGCACTCTTGGCTC
  TGGGGCTTTCGGACAGGTGGTGGAGGCCACAGCTCACGGTCTGAGCCATTCGCAGGCCACCATGAAAGTG
  GCTGTCAAGATGCTGAAATCGACAGCCAGAAGTAGCGAGAAGCAAGCCTTAATGTCCGAGCTGAAGATTA
  TGAGTCATCTTGGACCCCACCTGAACGTGGTCAACCTGCTGGGGGCCTGCACCAAAGGAGGGCCCATCTA
  CATCATCACGGAATACTGCCGATACGGTGATCTGGTGGACTACCTGCACCGGAACAAACACACCTTCTTG
  CAGCGACACTCCAACAAGCATTGTCCGCCCAGTGCTGAGCTCTACAGCAACGCCCTGCCAGTGGGGTTCT
  CCCTACCCAGCCACTTGAACCTGACTGGGGAGAGTGACGGTGGCTACATGGATATGAGCAAGGATGAATC
  TATAGATTACGTGCCCATGTTGGACATGAAAGGAGACATCAAATACGCAGACATTGAGTCCCCCAGCTAC
  ATGGCCCCTTATGATAACTATGTCCCATCTGCCCCTGAAAGGACCTATCGCGCCACCTTAATCAACGACT
  CACCAGTGCTCAGCTACACAGACCTCGTGGGCTTCAGCTACCAAGTGGCCAACGGCATGGACTTCTTAGC
  CTCTAAGAACTGTGTTCACCGAGACTTGGCGGCCAGGAATGTGCTCATCTGCGAGGGCAAGCTGGTCAAG
  ATCTGTGACTTCGGCCTGGCTCGAGACATCATGAGGGACTCAAACTACATCTCCAAAGGCAGCACCTTCC
  TGCCTCTGAAGTGGATGGCCCCAGAGAGCATCTTCAACAGCCTCTACACCACTTTGAGTGATGTCTGGTC
  TTTTGGGATCCTACTCTGGGAGATCTTCACACTGGGTGGCACCCCTTACCCAGAGCTGCCCATGAACGAC
  CAGTTCTACAATGCCATCAAGAGGGGCTACCGCATGGCCCAGCCTGCTCATGCCTCCGACGAGATCTATG
  AGATCATGCAGAAATGCTGGGAAGAAAAGTTTGAGACTCGACCCCCCTTCTCCCAGCTGGTGCTGCTCCT
  GGAGAGGCTTCTGGGTGAAGGCTATAAAAAGAAGTACCAGCAGGTAGATGAGGAGTTCCTGAGGAGTGAC
  CATCCTGCCATCCTGAGGTCCCAAGCCCGCTTTCCGGGGATCCACAGCCTCCGATCCCCTCTGGACACCA
  GCTCTGTTCTCTACACTGCCGTGCAGCCCAATGAGAGTGACAATGACTACATCATCCCCTTACCTGACCC
  CAAGCCTGACGTTGCTGATGAAGGTCTCCCAGAGGGGTCCCCCAGCCTTGCCAGTTCCACCTTGAATGAA
  GTCAACACTTCCTCCACCATCTCCTGCGACAGTCCCCTGGAGCTCCAAGAAGAGCCACAGCAAGCAGAGC
  CTGAGGCACAACTGGAGCAGCCACAGGATTCAGGCTGCCCAGGACCTCTGGCTGAAGCAGAGGACAGCTT
  CCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR225618 representing NM_008809
  Red=Cloning site Green=Tags(s)
  
  MGLPGVIPALVLRGQLLLSVLWLLGPQTSRGLVITPPGPEFVLNISSTFVLTCSGSAPVMWEQMSQVPWQ
  EAAMNQDGTFSSVLTLTNVTGGDTGEYFCVYNNSLGPELSERKRIYIFVPDPTMGFLPMDSEDLFIFVTD
  VTETTIPCRVTDPQLEVTLHEKKVDIPLHVPYDHQRGFTGTFEDKTYICKTTIGDREVDSDTYYVYSLQV
  SSINVSVNAVQTVVRQGESITIRCIVMGNDVVNFQWTYPRMKSGRLVEPVTDYLFGVPSRIGSILHIPTA
  ELSDSGTYTCNVSVSVNDHGDEKAINISVIENGYVRLLETLGDVEIAELHRSRTLRVVFEAYPMPSVLWL
  KDNRTLGDSGAGELVLSTRNMSETRYVSELILVRVKVSEAGYYTMRAFHEDDEVQLSFKLQVNVPVRVLE
  LSESHPANGEQTIRCRGRGMPQPNVTWSTCRDLKRCPRKLSPTPLGNSSKEESQLETNVTFWEEDQEYEV
  VSTLRLRHVDQPLSVRCMLQNSMGGDSQEVTVVPHSLPFKVVVISAILALVVLTVISLIILIMLWQKKPR
  YEIRWKVIESVSSDGHEYIYVDPVQLPYDSTWELPRDQLVLGRTLGSGAFGQVVEATAHGLSHSQATMKV
  AVKMLKSTARSSEKQALMSELKIMSHLGPHLNVVNLLGACTKGGPIYIITEYCRYGDLVDYLHRNKHTFL
  QRHSNKHCPPSAELYSNALPVGFSLPSHLNLTGESDGGYMDMSKDESIDYVPMLDMKGDIKYADIESPSY
  MAPYDNYVPSAPERTYRATLINDSPVLSYTDLVGFSYQVANGMDFLASKNCVHRDLAARNVLICEGKLVK
  ICDFGLARDIMRDSNYISKGSTFLPLKWMAPESIFNSLYTTLSDVWSFGILLWEIFTLGGTPYPELPMND
  QFYNAIKRGYRMAQPAHASDEIYEIMQKCWEEKFETRPPFSQLVLLLERLLGEGYKKKYQQVDEEFLRSD
  HPAILRSQARFPGIHSLRSPLDTSSVLYTAVQPNESDNDYIIPLPDPKPDVADEGLPEGSPSLASSTLNE
  VNTSSTISCDSPLELQEEPQQAEPEAQLEQPQDSGCPGPLAEAEDSFL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008809
• ORF Size: 3294 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008809.2, NP_032835.2
• RefSeq Size: 5410 bp
• RefSeq ORF: 3297 bp
• Locus ID: 18596
• UniProt ID: P05622
• Cytogenetics: 18 34.41 cM
• MW: 123.2 kDa
• Gene Summary: Tyrosine-protein kinase that acts as cell-surface receptor for homodimeric PDGFB and PDGFD and for heterodimers formed by PDGFA and PDGFB, and plays an essential role in the regulation of embryonic development, cell proliferation, survival, differentiation, chemotaxis and migration. Plays an essential role in blood vessel development by promoting proliferation, migration and recruitment of pericytes and smooth muscle cells to endothelial cells. Plays a role in the migration of vascular smooth muscle cells and the formation of neointima at vascular injury sites. Required for normal development of the cardiovascular system. Required for normal recruitment of pericytes (mesangial cells) in the kidney glomerulus, and for normal formation of a branched network of capillaries in kidney glomeruli. Promotes rearrangement of the actin cytoskeleton and the formation of membrane ruffles. Binding of its cognate ligands - homodimeric PDGFB, heterodimers formed by PDGFA and PDGFB or homodimeric PDGFD -leads to the activation of several signaling cascades; the response depends on the nature of the bound ligand and is modulated by the formation of heterodimers between PDGFRA and PDGFRB. Phosphorylates PLCG1, PIK3R1, PTPN11, RASA1/GAP, CBL, SHC1 and NCK1. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate, mobilization of cytosolic Ca(2+) and the activation of protein kinase C. Phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, leads to the activation of the AKT1 signaling pathway. Phosphorylation of SHC1, or of the C-terminus of PTPN11, creates a binding site for GRB2, resulting in the activation of HRAS, RAF1 and down-stream MAP kinases, including MAPK1/ERK2 and/or MAPK3/ERK1. Promotes phosphorylation and activation of SRC family kinases. Promotes phosphorylation of PDCD6IP/ALIX and STAM (By similarity). Receptor signaling is down-regulated by protein phosphatases that dephosphorylate the receptor and its down-stream effectors, and by rapid internalization of the activated receptor.[UniProtKB/Swiss-Prot Function]