Mark2 (NM_001080388) Mouse Untagged Clone

CAT#: MC204604

Mark2 (untagged) - Mouse MAP/microtubule affinity-regulating kinase 2 (Mark2), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Mark2
• Synonyms: Emk; EMK-1; Par-1; Par-1b
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC058556
  CAGAGCCGAACCTCGGTCTTCGGAATCTAGGAGGTGCGCGCGTCCCCGGGGATTACCTTCTTCGGTAGTT TCCGGAATTCACCCATCGTCTGGGCCGAGAGGAAAAGGCTCTTCCCCCTCCCGTTCCTAGTTGTTTCGGA TGTTTCCGGCTCTTGCCGGCGGAAAGAGCCGAGGGCTGGCGGTGGTGGCGGCCATGTTGGGAGCAGCAGG TCCGGCGGCGGCTGCCTGTGTGTCGGGCGCGGAGCAGTGCGGCTGAGGGCAGGGGAGGAGCGAGGCAGGC GGCCGGCTGCGGCGGTTGAGAGTAGGCGGAGCGGCGCGGCCGGGCCGAAAGGCGGCACAACCCAGCCGGG GGTCGGGGGGGGGTGCGGTCCGGAGCCGCTCGGAGCCGGCGAGGCCTAGCCCGAGCGGTGCATCACCGGG CTGGCGTGAGCCATCGCCCGGCCTCCCCGCACCCCCGGCCGGGGCCCATGCAGCGGGTGCTCCTGCTGTG AGAAGCCCCGCCCGGCCGGGCTCCTAGCCTTCCCTTCCCTATCTTCCTCCAAGCTTCTCCATTCCCTCCC CTGAGATACCGGCGCCATGTCCAGCGCTCGGACCCCCCTACCCACGCTGAACGAAAGGGACACGGAGCAG CCCACCTTGGGACACCTTGATTCCAAGCCCAGCAGTAAGTCCAACATGCTGCGGGGCCGCAACTCAGCCA CCTCTGCTGACGAGCAGCCCCATATTGGCAACTACCGGCTCCTTAAGACCATTGGCAAGGGTAACTTTGC CAAGGTGAAGTTGGCCCGGCACATCCTGACGGGGAAAGAGGTAGCTGTGAAGATCATCGACAAGACCCAG CTGAACTCCTCCAGCCTACAGAAACTGTTCCGAGAAGTAAGAATAATGAAGGTTTTGAATCATCCCAACA TAGTTAAGTTGTTTGAAGTGATCGAGACTGAGAAGACTCTCTACCTTGTCATGGAGTATGCCAGTGGCGG AGAGGTGTTTGATTACCTAGTGGCCCATGGCAGGATGAAAGAAAAAGAAGCTCGAGCCAAATTTCGCCAG ATAGTGTCTGCTGTGCAGTACTGTCACCAGAAGTTCATTGTTCATAGAGATCTAAAGGCAGAAAACCTGC TCCTGGATGCTGATATGAACATCAAGATTGCAGACTTTGGCTTTAGCAACGAATTCACCTTTGGGAACAA GCTGGATACTTTCTGTGGCAGTCCTCCTTATGCTGCCCCAGAACTTTTCCAGGGCAAAAAGTATGATGGT CCTGAGGTGGATGTCTGGAGCCTGGGAGTCATCCTCTATACACTGGTCAGCGGATCCCTGCCTTTTGATG GACAGAACCTCAAGGAGCTGCGGGAACGGGTACTGAGGGGGAAATACCGTATTCCGTTCTACATGTCCAC GGACTGTGAAAATCTGCTTAAGAAATTTCTCATACTTAATCCTAGTAAGAGAGGCACTTTAGAGCAAATT ATGAAAGATCGGTGGATGAACGTGGGGCATGAGGACGATGAGCTAAAGCCTTATGTGGAACCTCTCCCTG ACTACAAGGACCCCCGGCGGACAGAGTTGATGGTGTCAATGGGTTACACACGGGAAGAGATCCAGGACTC GCTGGTAGGCCAGAGGTACAACGAAGTGATGGCTACCTATCTGCTCCTTGGCTACAAGAGCTCTGAGCTG GAAGGTGATACCATCACTTTGAAGCCCCGGCCTTCAGCTGATCTAACCAACAGCAGTGCCCCATCTCCAT CCCACAAGGTTCAGCGCAGCGTCTCTGCCAACCCCAAGCAACGACGCTCCAGTGACCAGGCCGTCCCTGC CATTCCCACCTCGAATTCCTACTCTAAGAAGACTCAGAGTAACAACGCAGAAAATAAGCGGCCTGAGGAA GAGACAGGGCGGAAAGCCAGCAGCACCGCCAAAGTGCCTGCCAGCCCTCTGCCTGGCCTGGACAGGAAGA AGACCACTCCTGCCCCCTCCACGAACAGCGTCCTTTCCACCAGCACAAACCGAAGCAGGAACTCCCCACT TTTGGACAGGGCCAGCCTTGGCCAGGCCTCCATCCAGAATGGTAAAGACAGCACAGCCCCCCAGCGCGTC CCTGTCGCCTCCCCCTCCGCCCACAACATCAGCAGCAGTAGTGGAGCCCCAGACCGAACTAATTTCCCAC GGGGTGTGTCCAGTCGAAGCACCTTCCATGCTGGGCAGCTCCGACAGGTGCGGGACCAGCAGAATCTACC CTACGGTGTGACCCCAGCCTCTCCCTCTGGCCATAGCCAGGGCCGGCGGGGGGCCTCTGGCAGCATCTTC AGCAAGTTCACCTCCAAGTTTGTCCGCAGGAACCTGAATGAACCTGAAAGCAAAGACCGAGTGGAGACGC TCAGACCTCACGTGGTAGGCAGTGGAGGCACTGACAAGGACAAGGAGGAGTTTCGGGAGGCCAAGCCTCG CTCCCTGCGCTTCACCTGGAGCATGAAGACCACGAGCTCTATGGAGCCCAATGAGATGATGCGGGAGATC CGCAAGGTGCTGGACGCCAACAGCTGCCAAAGCGAGCTGCACGAGCGGTACATGCTACTGTGCGTGCATG GCACACCAGGCCACGAGAACTTTGTGCAGTGGGAGATGGAGGTGTGCAAACTGCCCCGGCTGTCTCTCAA CGGTGTTCGGTTTAAGCGGATATCGGGCACTTCCATGGCCTTCAAAAACATTGCCTCCAAAATAGCCAAT GAGCTGAAGCTTTAACAGGCTGTCAGGAGGGGCAGCAGAGGCCCACCAGTGGACTTGGCTGCTTGGGCCA ACCCTGCATCCCCCTGGGAGAAACTGCAGGATGAATTGGTGTGTCTCCCCTGCTGGCACTTGTCCCCTTC CAGGCCCTTCTCAGTTTTTTTCTTCCATGTTTGTGGGGGATGGGAAGTGGTTCTTCTCCCTCCCACATTC ACCCCTGCCCAGAAGTCCCTCCCTTCCCCCCTGCAGGAGGCAAAGGAAGGGGAGGGGGCTGGGGGGGGCA GGGCTCCCCCTCGGTACTGCGGTTGCACAGAGTATTTCGCCTAAACCAAGAAAATTTTTATTCCCAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_001080388
• Insert Size: 2169 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC058556, AAH58556
• RefSeq Size: 3106 bp
• RefSeq ORF: 2169 bp
• Locus ID: 13728
• UniProt ID: Q05512
• Cytogenetics: 19 5.32 cM
• Gene Summary: Serine/threonine-protein kinase. Involved in cell polarity and microtubule dynamics regulation. Phosphorylates CRTC2/TORC2, DCX, HDAC7, KIF13B, MAP2, MAP4 and RAB11FIP2. Phosphorylates the microtubule-associated protein MAPT/TAU. Plays a key role in cell polarity by phosphorylating the microtubule-associated proteins MAP2, MAP4 and MAPT/TAU at KXGS motifs, causing detachment from microtubules, and their disassembly. Regulates epithelial cell polarity by phosphorylating RAB11FIP2. Involved in the regulation of neuronal migration through its dual activities in regulating cellular polarity and microtubule dynamics, possibly by phosphorylating and regulating DCX. Regulates axogenesis by phosphorylating KIF13B, promoting interaction between KIF13B and 14-3-3 and inhibiting microtubule-dependent accumulation of KIF13B. Also required for neurite outgrowth and establishment of neuronal polarity. Regulates localization and activity of some histone deacetylases by mediating phosphorylation of HDAC7, promoting subsequent interaction between HDAC7 and 14-3-3 and export from the nucleus. Also acts as a positive regulator of the Wnt signaling pathway, probably by mediating phosphorylation of dishevelled proteins (DVL1, DVL2 and/or DVL3). Modulates the developmental decision to build a columnar versus a hepatic epithelial cell apparently by promoting a switch from a direct to a transcytotic mode of apical protein delivery. Essential for the asymmetric development of membrane domains of polarized epithelial cells.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks an in-frame exon in the 3' coding region, compared to variant 1. The encoded isoform (2) is shorter, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.