Dctd (NM_178788) Mouse Untagged Clone

CAT#: MC204575

Dctd (untagged) - Mouse dCMP deaminase (Dctd), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Dctd
• Synonyms: 6030466N05Rik
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC031719
  CGGACGCGTGGGCGCGCACGGCGGCTGTGTGGGGTGGACGGTGCGAGCGTGGTCTGCACCTCGAAGGAGC GGACGTGACGTATGACTTCTACACCCAACATGAGTGACATTTCCTGCAAAAAACGGGATGACTATCTGGA ATGGCCCGAGTATTTCATGGCAGTGGCCTTCTTGTCGGCACAAAGAAGCAAGGACCCAAGTTCCCAGGTG GGGGCCTGCATTGTGAACACAGAAAACAAGATTGTTGGGATCGGGTACAATGGCATGCCAAATGGGTGCA GTGATGACCTGTTGCCTTGGAGGAGAACAGCAGAAAATAAGCTGGATACTAAATATCCTTATGTGTGCCA TGCCGAGCTGAACGCCATCATGAACAAGAACTCGGCTGATGTGAAGGGCTGCAGCATGTACGTCGCCTTG TTCCCGTGTAACGAATGTGCTAAACTCATCATCCAGGCAGGTATAAAAGAGGTTATCTTCATGTCGGATA AATACCACGACAGCGAGGAGACGACGGCTGCTAGGCTCCTGTTTAAGTTGGCTGGGGTTACGTTCAGAAA ATTCACACCGAAGTACAGCAAGATTGTCATTGACTTTGATTCGATCAATAGCCGACCGAGTCAGAAGCCT CAGTGAGTTTCGTCTCGTTAAAGCCCCAGAAGAAGATGACACCCCACCACGTGCGTGTGCACACACACAC ACACACACACACATACACACAAGGGGCTCCCCAAACCTTTCATCTTGAAGTCCCACGGAACTTTTTAATA GCACAACACTAAACATCTAAAGAATATAAAACTTGGAATCTTCTCACCCGTCACGTGGAGCTCCATCTGT TTCAGCTGTTGTTGCAGTTTGGTTTGGAAACTAAGGGACCTGCCCACAGGAGCTGGAGTGAAGGTCTCTC CCCTTCCAGCCCGTCTGCCAGAGAGTGTCAGCTCTGTCATCCTCCATATTTCGACAGCCGCTCCTTGCTG CTACACGGGGTAGGAGGACATACAAATAATTGATTCTCGTTCTTAGCAGGGATGAACAGGCACACTGAGG TGAACATCTGGCCCAAATGAAGCATACCCTGTCACGCCCTTGGGAAAACAAATAGGACCGAACAAAGCTA CATGAACACACCTGCTGTAGAGTTGAGGGGACCTCTCACCTGGTCACATGAGGTTGAGGAGGGCCTAGTG TCCTGGCAGATGATCAGTACAGTGTCCCCCTCAAGTCACACTTTCCTGCCTTACACAGAGCAGTAGCATA CCATCCCTCTGGAGGGTAGGGGACCCCTGCGGTTCCAGCCTCTTAGCCTTGGTTTGTTGGTCACATAGCA CTGTTAAATTTATCCGAGGCATAAGTAGTTCCTAGAGGAGACAGCCTATCACTCCCCCTAGACCAGGCGC TTTGCGGTGACAGCTGGAGCTATATGGATGGGTGATGAGAGACGTTCCCCCATCCCGTGTCTCCTGCTGT GTGGACACATGTCACTCCTGCTCCACATGCCAACATGGTTCATTTCCAAGGGCACCCTATAACCAGAGAG CACATTAATTCCTGTCCTATAAGAGGGCTGTGGGAAACTGTGTACCTGGAGGTGGTCCATGCCAGATCCT CATTTGTGCCTTCAGCTTCATAGGCAGCATACGCCTGCTGCCCCATTGTGGGTGGGGGTGCCCCACTGCG TACTCTGTTTTTAATTTCATTTAACTTCTAGCAGACATGCTCAGTGTCTTTGATAAAATAGTGTATTTTT TCATGTGTTTTTATTTCATTTCTAAGATTTTTTTTTAACACTCCGTATTCTGGAGCATTTTTTTACAATT TGGATCTCTGCCAGTATTGTTAAAATTTTATTAAAATCATCCAGCACCAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_178788
• Insert Size: 537 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC031719, AAH31719
• RefSeq Size: 1923 bp
• RefSeq ORF: 537 bp
• Locus ID: 320685
• UniProt ID: Q8K2D6
• Cytogenetics: 8 B1.1
• Gene Summary: Supplies the nucleotide substrate for thymidylate synthetase.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcript. Variants 1, 2 and 3 encode the same protein. CCDS Note: The coding region has been updated to extend the N-terminus. The use of an alternative upstream start codon, which appears to be rodent-specific, would result in a protein that is 6 aa longer at the N-terminal.