Cnot3 (NM_146176) Mouse Untagged Clone

CAT#: MC204447

Cnot3 (untagged) - Mouse CCR4-NOT transcription complex, subunit 3 (Cnot3), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Cnot3
• Synonyms: A930039N10Rik
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC030332
  CCACGCGTCCGGATCCCTGAATCCCCTACTCGGTCGTCTAGAACAATCCCGGGCCCACTTCCTCTATCAA CAACCCAACCCTACTTCCGGCTTTCGCGCCCTCTTATCGCGATAGCGCCCGGGCTCGGGGCGCGAGGAAA GAGGAAAAAAAAAAAAAAAAAAAGGCGGCGGGCGGGCGCTCGCCTCTCTCTCCCCCCTCCTCGCCTGTCG CGATACGCTCCGCAGCGGCGGCGCCAGCTCCTGCGCGGCCATTTTCAAGAGAGTATGAAGAGAGTGCGTC TGTAGGGCAGGGAAGATGGCGGACAAGCGCAAACTCCAAGGTGAGATTGATCGCTGCCTCAAGAAGGTGT CCGAAGGGGTGGAGCAGTTTGAAGATATTTGGCAAAAGCTCCACAATGCAGCCAACGCGAACCAGAAAGA AAAGTATGAGGCTGACCTAAAGAAGGAGATTAAGAAGCTACAACGGCTGAGGGACCAGATCAAGACATGG GTAGCATCAAATGAGATCAAGGACAAAAGGCAGCTTATTGAAAACCGCAAGCTCATTGAGACGCAAATGG AACGGTTCAAAGTTGTGGAACGAGAGACCAAAACCAAAGCGTATAGCAAGGAGGGTCTGGGTCTGGCTCA GAAGGTGGATCCTGCCCAGAAGGAGAAGGAAGAAGTTGGCCAGTGGCTCACGAACACCATTGACACCTTA AATATGCAGGTGGACCAGTTTGAGAGTGAAGTGGAGTCACTGTCGGTACAGACACGCAAGAAGAAAGGCG ACAAGGATAAGCAGGACCGGATTGAGGGCTTGAAGCGGCATATCGAGAAGCACCGCTACCATGTTCGTAT GCTGGAGACCATCCTGCGAATGCTGGACAATGACTCCATCCTGGTTGATGCCATCCGCAAGATCAAGGAT GATGTGGAGTACTACGTTGACTCATCCCAGGACCCCGACTTTGAAGAGAACGAATTCCTCTATGACGACC TGGACCTCGAGGACATTCCACAGGCGCTGGTCGCCACCTCCCCCCCCAGCCACAGCCACATGGAGGACGA GATCTTCAACCAGTCTAGCAGTACACCCACCTCAACAACCTCCAGCTCTCCCATCCCACCCAGCCCAGCA AACTGCACTACGGAAAACTCTGAAGATGATAAGAAGAGAGGCCGATCTACAGATAGTGAAGTCAGCCAGT CTCCAGCCAAAAATGGCTCCAAGCCTGTCCACAGCAACCAGCACCCCCAGTCCCCAGCTGTGCCGCCCAC CTACCCCTCTGGCCCCCCACCTACCACTTCTGCCTTGAGCTCCACCCCTGGCAACAATGGGGCCTCTACC CCAGCAGCACCTACAAGTGCCCTGGGCCCTAAGGCCAGTCCAGCTCCCAGCCACAACTCGGGTACTCCTG CACCCTATGCCCAGGCTGTGGCCCCACCTAATGCCAGCGGGCCCAGCAATGCCCAGCCACGGCCCCCCAG TGCCCAGCCAAGCGGGGGAAGTGGTGGTGGCAGCGGAGGGAGCAGCAGCAATAGTAACAGTGGCACAGGC GGAGGGGCTGGCAAGCAGAACGGTGCCACAAGCTATAGTTCGGTTGTGGCAGACAGCCCTGCAGAGGTGA CCCTGAGTAGCAGTGGGGGCAGCAGTGCCAGCAGCCAAGCCCTTGGGCCCACTTCGGGCCCTCACAACCC AGCTCCCAGCACCTCAAAGGAATCCAGTACGGCAGCCCCATCAGGGGCTGGGAATGTGGCTTCAGGCTCA GGGAATAACTCGGGGGGACCCAGCCTCTTGGTGCCACTGCCTGTAAATCCCCCCAGTTCTCCAACGCCCA GCTTCAGTGAAGCCAAGGCAGCTGGTACCCTGCTTAATGGTCCTCCACAGTTCAGCACCACCCCAGAAAT CAAGGCCCCTGAACCACTCAGCTCTCTGAAATCCATGGCAGAACGGGCAGCTATCAGCTCTGGTATTGAG GACCCTGTGCCAACGTTACACCTAACTGATCGAGACATCATCCTGAGCAGCACATCAGCACCACCCACCT CATCCCAGCCACCCCTGCAGCTGTCAGAGGTGAACATACCATTGTCACTGGGTGTCTGTCCACTGGGGCC AGTTTCCCTCACCAAGGAGCAGCTATACCAACAGGCCATGGAAGAGGCCGCCTGGCACCACATGCCCCAC CCCTCTGACTCCGAGCGCATTCGGCAGTACCTCCCCCGGAACCCTTGCCCGACACCCCCCTACCACCACC AGATGCCACCCCCACACTCGGACACTGTGGAGTTCTACCAGCGCCTGTCAACTGAGACGCTCTTCTTCAT CTTCTACTATCTGGAGGGAACCAAGGCACAGTACTTGGCAGCCAAGGCCCTAAAGAAGCAGTCCTGGCGA TTCCACACCAAGTATATGATGTGGTTCCAGAGGCACGAGGAGCCTAAGACCATCACAGATGAGTTTGAGC AGGGCACCTACATCTACTTTGACTACGAGAAGTGGGGCCAGCGGAAGAAGGAAGGCTTCACCTTTGAGTA CCGCTACCTGGAGGACCGGGACCTCCAGTGACACCGGCCCCCCTCTACCCTCTCCCTCCCTCGCATGCTG ATCCCCCTGCCCAGGTGAGGGCCCTGCCCTGGAAGACTGGGGGAGGCCCAAGGCCATGGGACACCCTCCC CCCCAGGAAGCAGGGAAGGGATAGGGAGGTTTTCTCTTCCTCTCTGCCCTACCCTGGGGGCCTGGGGGCA AGGGCTGCCCCCTCCTCCCCTCCCCAGTGAGGGACATTTTTTGGTAAACCTATTTTCATTTTGGAAAATA TTTATGAATAAATAGTTTTATATGAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_146176
• Insert Size: 2256 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC030332, AAH30332
• RefSeq Size: 2849 bp
• RefSeq ORF: 2256 bp
• Locus ID: 232791
• UniProt ID: Q8K0V4
• Cytogenetics: 7 A1
• Gene Summary: Component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. Additional complex functions may be a consequence of its influence on mRNA expression. May be involved in metabolic regulation; may be involved in recruitment of the CCR4-NOT complex to deadenylation target mRNAs involved in energy metabolism. Involved in mitotic progression and regulation of the spindle assembly checkpoint by regulating the stability of MAD1L1 mRNA. Can repress transcription and may link the CCR4-NOT complex to transcriptional regulation; the repressive function may involve histone deacetylases. Involved in the maintenance of embryonic stem (ES) cell identity; prevents their differentiation towards extraembryonic trophectoderm lineages.[UniProtKB/Swiss-Prot Function]