Bsnd (NM_080458) Mouse Untagged Clone

CAT#: MC204433

Bsnd (untagged) - Mouse Bartter syndrome, infantile, with sensorineural deafness (Barttin) (Bsnd), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Bsnd
• Synonyms: MGC47291
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC038287
  CCACGCGTCCGGGCAGGAAGAAAGTTAGATGGCTGGGACCTTGGGCCCACTGAAGCGTGTCACCTGCCCT GCTGGGCTCAGGGCTGAGAGCTGGGCCTGTGGAGTTTGCTCCTTGCTCTGTGCATCATCACCTTGGTTCT CAGCCATCTCCTGGAGCATCCTTCCTTGAGAGCCTGCTCAGCGTTTGAGGAGGATTGCGGGTAGTCCCCT GCTGGTGTGCTGATCAGCTAGCTACCAGGGCTGGTTAGCCACCATGGCTGATGAGAAGACCTTCCGAATC GGCTTCATCGTGCTGGGCCTCTTCCTGCTATCCCTGGGTACATTCCTTATGAGCCACGATCGACCCCAGG TCTACGGCACCTTCTATGCCATGGGCAGCGTCATGGTGATCGGGGGTGTCATCTGGAGCATGTGCCAATG CTATCCTAAGATCACCTTTGTGCCAGCAGACTCTGACTTCCAAGGCATCTTGTCCCCAAAGGCCCTGAGC CTGCTGGAGACCGGGCTTTCGGAGGTAAAGAGCCCCCAGCCCCCCTACGTCAGGCTGTGGGAAGAAGCCG CCTATGACCAGAGCCTCCCAGACTTCACTCACATCCAAATGAAGGTCATGGGCTACAGTGAGGACCCCAG GCCACTGCTGGCCCCCGAGCTGAAGACAGGAGCCAGCAGTGTCAGGGAAGGCGAGCCTCGTACTGCTCAG GCCTGGATGGAGGCTCCGGTGGTCGTCCATAGAGGGTCAGATGAGAACGAGGGAGAGAAATCCCATTCTC AGAGCAGCCCTTCAGTGGGTCCCCAAGGCTCTGCACCCTTGGCCTCATTCCATGACGACCTGGACGTGGG CTCCAGCGAAGGCAGCAGCCTGCAACCATCCCCCAACAGGGATGAGCCACACCGGCAGGTGCCCTGGGCC AGCAGGGGTCCACTGGATCGCTTCAGTGACTTTGCTCTGATTGACGACACCCCTACATCTGAGGACACGG TCCTGGACGGGCAGGCACGGGAGGCAGCGCTACCCAGAAAGCAGCAATGGTCCCTGAGGATGAAGGGGGA GACTGTCCAGGCACGCGCAGAAGAACCGGAGCAGGAGGAGGAAGATCTGTATTACGGGCTGCCAGACAGC CCTGGGGACCCCCTACCCGACAAAGAGTTGGGCTTCGAGCCTGACATCCAGGGCTGAGATGAAACTGCCT CCTGTGTCTAGCCTGACAGCACTGCTGTCTTCATGTGACCCCTCAAGGGCTCAGTTTTCCAAGTCCCAAA CAGGATTGTATGGAGGACCAAGTGAGGCAGTGAACTGAAGCATCTTGGGAACTGAAAGGAACACACATGG GGGGCAGCAATCCTCCCCCCTGCGAGCCACATGCGTCTGGTTGTAGCTTGTTCTGCTGACCTGCAGGGAC AGTGGGGTTTTCTGAGGTCCTGTGTGACAGCCAGACCCCCAGAAGCCCTTCTCCATCTCCTAATGCTAGC TTCTGATTGGGCCTCCTGTGGTGCTACCCAGGGCCTCTGAGAACCCCATGTCCACTGTGTTTTGGTCCTC TGATGAGTGTTGGACTCACGCACCCCTCTCAGAGCCGATGCTGTCTCCTGGCTCAGTGTCTTTAGCAGTT TCTCTGCTCTGGGATCTATGACTCTCTGTGGCTGACTTCTGCTGAGCTGAGCTGACCTCTGGATATTACT CTAGTCCCTCCTGTTCATGCCCCTAGCCCATGCCTATACCCTTGCTTGAGCTGTGTTCTTCCAGGGCCCC TTGTGCTGAGTGAACTCCCACCCATTCCTATACACCCACCAAAGTGACCTTTCTCCTGCTAGTTTTCCTG GCATCCCAACCCCTAGATGAGAATTTCCCTCTGGGGCTTGTGTGTCCATTTCTCTGGCTGGTGCTTGCCA CTGCCCAGGGACAGCATCTTGACTTCATTCACCAGAGTTCCCAGGAGACCTTTGGCGTATGCAGATCTCA GCAGATCTTGGAACCTTTGCAGATAGGGTCACCCTCCCCAGTTTTGACAAGTATGGACTCTTAGTCTCTG GCACCCACTTTTCAAAATGGCTTCTAGACAGTCATAAAAACCAGGCGAAAAGCTACCAGAAGAGAACCCA ACAACAACTCACAAAGCACACTTGCATTGGTTTGGAATGGACAAAATCCAGCTGCTGAAGTAATTCCACT TGGTGCCCTTGGCACTTACTGAGCATCTGCTATGTGCCAGGCTCAGCTTGGAAAACAGTCATTGGTCCCT GACCCTGTAAAGGGCTCATGGTCTGAGCTGGCTGTCTTGTAAGCTGGGAACACAGCATGGAAGGTGGATG GGAGCAGAGTCACTCTCCTGTCTGCAGGTCTTGCAGAACCCTCTGTTCTCAGCCCAGCATGGAAGGAGCC ATAAGGAGAAAGGTGTTGCTGCTTTCTCCAGCCAGGTCTACACAACTTAGACCTGTTCTGAGATGTCTAA GAGCAGGGCACTGGGAGAAGGTTAGGGGTCTCAGCCGAGGGAAGGAGATGCCCACCGGTGCTCACTGAGA ACACACCACAACCCTCCCCATGTCTGGGGCATTTCTACCTCTTCAGTGAAGACGCCTCCACCAGGCACCT CCTCTCTGCTGATAGGTCACAGACCAGGTGTCCCTGCTCCTGTCTCAGCCAAGGGTGAGGTGACATGTAG CTGCTGTCTGTGTACTGCTGGCCCAGGCAAGGACTGACTGTGACCCACATCAGACAGAGCAAAGTCAGCA CCAGGGGATGAAATAAAAAAGTTTTTTAACTTCAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_080458
• Insert Size: 924 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC038287, AAH38287
• RefSeq Size: 2789 bp
• RefSeq ORF: 924 bp
• Locus ID: 140475
• UniProt ID: Q8VIM4
• Cytogenetics: 4 C7
• Gene Summary: Functions as a beta-subunit for CLCNKA and CLCNKB chloride channels. In the kidney CLCNK/BSND heteromers mediate chloride reabsorption by facilitating its basolateral efflux. In the stria, CLCNK/BSND channels drive potassium secretion by recycling chloride for the basolateral SLC12A2 cotransporter.[UniProtKB/Swiss-Prot Function]