Sirt3 (NM_022433) Mouse Untagged Clone

CAT#: MC204209

Sirt3 (untagged) - Mouse sirtuin 3 (silent mating type information regulation 2, homolog) 3 (S. cerevisiae) (Sirt3), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Sirt3
• Synonyms: 2310003L23Rik; AI848213; Sir2l3
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC025878
  CGCTAAGCGGTCGACTGGCATTGGCCGCGCTCAGACTGTGGGGTCCGGGAGTGTTACAGGTGGGAGAAGG CCCATATCCCTCTGTGTGGGAGCCTCAGGCGGCTTTGGAGGTGGAGGAAGCAGTGAGAAGAAGTTTTCTC TGCAGGATGTAGCTGAGCTGCTTCGGACCAGAGCCTGCAGTAGGGTGGTGGTCATGGTGGGGGCCGGCAT CAGCACACCCAGTGGCATCCCGGACTTCAGATCCCCAGGGAGCGGCCTCTACAGCAACCTTCAGCAGTAT GACATCCCGTACCCTGAAGCCATCTTTGAACTTGGCTTTTTCTTTCACAACCCCAAGCCCTTTTTCATGT TGGCCAAGGAGCTGTACCCTGGGCACTACAGGCCCAATGTCACTCACTACTTCCTGAGGCTCCTCCACGA CAAGGAGCTGCTTCTGCGGCTCTATACACAGAACATCGACGGGCTTGAGAGAGCATCTGGGATCCCTGCC TCAAAGCTGGTTGAAGCCCACGGGACCTTTGTAACAGCTACATGCACGGTCTGTCGAAGGTCCTTCCCAG GGGAAGACATATGGGCTGATGTGATGGCGGACAGGGTGCCCCGCTGCCCTGTCTGTACTGGCGTTGTGAA ACCCGACATTGTGTTCTTTGGGGAGCAGCTGCCTGCAAGGTTCCTACTCCATATGGCTGACTTCGCTTTG GCAGATCTGCTACTCATTCTTGGGACCTCCCTGGAGGTGGAGCCTTTTGCCAGCTTGTCTGAAGCAGTAC AGAAATCAGTGCCCCGACTGCTCATCAATCGAGACTTGGTGGGGCCGTTCGTTCTGAGTCCTCGAAGGAA AGATGTGGTCCAGCTAGGGGATGTAGTTCATGGTGTGGAAAGGCTGGTGGACCTCCTGGGGTGGACACAA GAACTGCTGGATCTTATGCAGCGGGAACGTGGCAAGCTGGATGGACAGGACAGATAAGACTATGGCTTCT TCACCTGGGGAAGTCACACAGCAGATCATCCTATGTCCAGCAAGACTTCATGCCTGAAGACAGCTCCAAC ACGTTTACAAACATGAACCAGACCACAACATGTGGCCTGGACAGTGGTCCTCCGAGGCTGCCTTTGGAAA GGCTGACCAGGGATGTCTACCCTTGGGGCCCCTCCATGTGTGCGCCCTGTCCACCTCATCACTGCTGAAG GTGTAGTGCAGGTGCTGCTTTCTGCAGCGGCCCTTAAGTTATCACGAGGGCAGCACAGCACGCCCGTCGC CAGGCAGGCGATGCACTAGGGCAATCTAGCATGTTGATCGGTAAAGTGGCATCTTTAACTACAACATCAT TTCTTGCATGAAATAAACTTAGTATAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_022433
• Insert Size: 774 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC025878, AAH25878
• RefSeq Size: 1372 bp
• RefSeq ORF: 774 bp
• Locus ID: 64384
• UniProt ID: Q8R104
• Cytogenetics: 7 F4-F5
• Gene Summary: NAD-dependent protein deacetylase (PubMed:23835326, PubMed:17923681, PubMed:18794531, PubMed:21172655). Activates or deactivates mitochondrial target proteins by deacetylating key lysine residues (PubMed:23835326, PubMed:17923681, PubMed:18794531, PubMed:21172655). Known targets include ACSS1, IDH, GDH, PDHA1, SOD2, LCAD, SDHA and the ATP synthase subunit ATP5PO (PubMed:16790548, PubMed:18794531, PubMed:21172655). Contributes to the regulation of the cellular energy metabolism (PubMed:23835326). Important for regulating tissue-specific ATP levels (PubMed:18794531, PubMed:24252090). In response to metabolic stress, deacetylates transcription factor FOXO3 and recruits FOXO3 and mitochondrial RNA polymerase POLRMT to mtDNA to promote mtDNA transcription (PubMed:23283301).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) has an alternate 5' exon and an additional segment, resulting in a downstream AUG start codon, as compared to variant 3. The resulting isoform (1) is shorter at the N-terminus, as compared to isoform 2.