E2f4 (NM_148952) Mouse Untagged Clone

CAT#: MC204172

E2f4 (untagged) - Mouse E2F transcription factor 4 (E2f4), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: E2f4
• Synonyms: 2010111M04Rik; AI427446
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC026649
  CGACTAGGAACGGAAGCGGAAGTGGCGCCGCCGCCGGCCTCGCCTGGCCTGGCTGAGGGGAGGCGGCGGG CGGGCGCGATGGCGGAGGCCGGGCCACAGGCGCCGCCGCCCCCGGGGACTCCAAGCCGGCACGAGAAGAG TCTGGGACTTCTCACCACCAAGTTCGTGTCGCTCTTGCAGGAAGCCAAGGACGGCGTGCTTGACCTCAAG CTGGCAGCCGACACTCTTGCTGTGCGCCAGAAACGGCGGATCTACGACATCACCAACGTGCTGGAAGGCA TCGGTCTGATCGAGAAGAAATCCAAGAACAGCATCCAGTGGAAGGGCGTCGGGCCAGGTTGCAATACCCG GGAGATCGCTGACAAGCTGATTGAGCTCAAGGCAGAGATCGAGGAGCTGCAGCAACGAGAGCAAGAACTG GACCAGCACAAGGTGTGGGTGCAGCAGAGCATCCGGAATGTCACTGAGGACGTCCAGAACAGCTGCTTGG CCTACGTGACTCATGAAGACATCTGCAGATGCTTTGCTGGAGATACCCTCCTTGCCATCCGGGCGCCATC GGGCACCAGTCTAGAGGTGCCCATCCCAGAGGGTCTCAATGGCCAGAAGAAGTACCAGATTCACTTAAAG AGCATGAGTGGGCCTATCGAGGTGCTGCTAGTGAACAAGGAGGCCTGGAGTTCACCACCTGTGGCTGTGC CTGTCCCTCCCCCTGACGATCTACTCCAGAGTCCACCTGCTGTTTCTACACCTCCACCTCTGCCCAAGCC TGCCTTAGCTCAACCCCAAGAATCCTCTCCTCCAAGCAGCCCCCAGCTAACTACCCCCACTCCTGTCCTT GGCAGCACTCAAGTCTCAGAGGTGGCATGCCAGACATCTGAGATTGCAGTGAGTGGTAGCCCTGGAACTG AGAACAAGGACAGTGGTGAAGTCAGCTCACTCCCACTGGGCCTGACAGCACTGGACACTCGGCCTCTGCA GTCCTCTGCACTGCTGGATAGTAGTAGCAGCAGCAGCAGCAGCAGCAGTAGCAGCAGCAGCAGTTCATCT GGACCCAACCCTTCTACCTCCTTTGAGCCCATCAAAGCAGACCCCACAGGCGTTCTGGATCTCCCCAAAG AGCTGTCAGAAATCTTCGACCCCACGAGAGAGTGCATGAGCTCCGAGCTGCTGGAAGAACTGATGTCTTC AGAAGTGTTTGCCCCCCTCCTCCGACTTTCTCCACCTCCCGGAGACCACGATTACATCTACAACCTGGAC GAGAGTGAAGGTGTCTGTGATCTCTTTGATGTTCCTGTTCTCAAACTCTGACTGACAGGGACATGTCCTG TGCAGCTGCAACCCAGACTGTCTGGGCCTGGAGCCAACTAGGGACTGCCCCCCACCCCCACCCTAACCCC AAGAGCCCAACTCTTGGCTTACTCAGCCTTTTCTGGTCGCTCAGTTCGGGCTGTGCTCTCCTGTCTTGCA CTGGCACTTGTGACTGTGCTAGCATAGCTGGGCAGAGAGCTTAGCCCTCTCTACATGGAGCCTTCCCCAG CCAGGCTCAGCTGCCCCTCAGTGGCACCTCTGCCTTGTTCCTACCAGTCACTATGAAGGAGGTTCAGAGA GAGGGTGGTGCTGGGTGGTTGCCTACCATCACCTTTCCTTTCCCACCTGCTCACCCTGCAGCCTCTGGAC ATCTCGGATGCTCCCTGAGAGTCCTGGGATCTCTGGGCAGTCATGGTGGAGGGGGTTAATAAGGAATTGC TGTGTCTAGCTAGGAGTCTGGGAGGGTTGGTACCTAAGAATGGCTCCAGCTCCTGCCCACCCACCCCCAT CTCCCTTCACTTCCACTTATTCACTTACCCTCATTTAGAGCCATTGCAGAGATTTAGAAAGATTTACAGT AACGAATGAATTCCTATATAAAGATTATTTTTATACTTTTTTCAACAAAAGGAAATGTAATATTTGTACA GCATTCAAGTGAATAAAGACATGCCTAAGGCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_148952
• Insert Size: 1233 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC026649, AAH26649
• RefSeq Size: 2024 bp
• RefSeq ORF: 1233 bp
• Locus ID: 104394
• UniProt ID: Q8R0K9
• Cytogenetics: 8 D3
• Gene Summary: Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F4 binds with high affinity to RBL1 and RBL2. In some instances can also bind RB1. Specifically required for multiciliate cell differentiation: together with MCIDAS and E2F5, binds and activate genes required for centriole biogenesis.[UniProtKB/Swiss-Prot Function]