Dimt1 (NM_025447) Mouse Untagged Clone

CAT#: MC203899

Dimt1 (untagged) - Mouse DIM1 dimethyladenosine transferase 1-like (S. cerevisiae) (Dimt1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Dimt1
• Synonyms: 1500031M22Rik; AV111347; Dimt1l
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC019799
  CACGCGGTTCCCGGCTGCTTTCGCTTGGCACAGGGACTCCTCCGAGAAGATGCCGAAGGCCAAGTCCGCA GCGAGTAGCCGCCGGCGGGATCGTCAGGAGCAGCGCCGGGAGCTGAAGCGAGCTGGAGGACTCATGTTCA ACACAGGGATCGGACAACACATTTTGAAAAATCCTCTAATTGTTAACAGCATCATCGATAAGGCTGCTTT AAGACCTACCGATGTGGTGCTGGAAGTTGGGCCTGGGACTGGCAATATGACTGTCAAGCTGTTAGAAAAG GCCAAAAAGGTAGTTGCCTGTGAACTTGATCCAAGGCTAGTAGCTGAACTTCACAAAAGAGTTCAGGGCA CGCCTCTGGCCAGCAAACTCCAGGTCCTGGTGGGAGATGTACTGAAATCAGATTTGCCATTCTTTGATGC TTGTGTGGCAAACTTGCCTTATCAGATCTCCTCTCCCTTTGTCTTCAAGTTGCTGCTCCACCGGCCATTT TTCAGATGTGCTATACTTATGTTTCAGAGAGAGTTTGCTCTTCGATTGGTTGCCAAGCCTGGAGATAAAT TATACTGTAGACTCTCCATCAACACACAGCTTTTAGCACGCGTGGACCATCTAATGAAGGTGGGGAAGAA TAACTTCAGACCGCCCCCCAAGGTGGAGTCCAGCGTGGTCAGGATAGAACCTAAGAATCCGCCACCACCA ATCAATTTTCAGGAATGGGACGGCTTAGTAAGGATCACTTTTGTTCGGAAAAACAAGACACTGTCCGCTG CATTCAAATCAAGTGCAGTACAACAACTGTTGGAAAAAAATTACAGAATTCACTGTTCAGTACAGAATAC TGTAATACCAGAAGATTTCAGTATAGCAGATAAAATACAGCAAATCCTAACCAGCACAGGTTTCAGTGAC AAACGGGCCCGTTCCATGGACATAGATGACTTCATCAGGTTGCTACATGGATTCAATGCAGAAGGCATCC ACTTCTCCTAGCCATTTGAAAACTATTTTTTCAAGACCAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_025447
• Insert Size: 942 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC019799, AAH19799
• RefSeq Size: 1035 bp
• RefSeq ORF: 942 bp
• Locus ID: 66254
• UniProt ID: Q9D0D4
• Cytogenetics: 13 D2.1
• Gene Summary: Specifically dimethylates two adjacent adenosines in the loop of a conserved hairpin near the 3'-end of 18S rRNA in the 40S particle. Involved in the pre-rRNA processing steps leading to small-subunit rRNA production independently of its RNA-modifying catalytic activity.[UniProtKB/Swiss-Prot Function]