Ss18l1 (NM_178750) Mouse Untagged Clone

CAT#: MC203600

Ss18l1 (untagged) - Mouse synovial sarcoma translocation gene on chromosome 18-like 1 (Ss18l1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ss18l1
• Synonyms: A230053O16Rik; CREST
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC053087
  GGCGACAGGCAGGCGGGCGAGCCCACGGCACCGCGCCCCCCGTGTCCCCGCCCCCGCTCTGCGGAGAATG GGCACCTCGGGCCGCGGGGCGCAGCCGGAGAATAAACCCCAATGATCACGGGCTGAGTCCGCGCCACCAC CATGTCCGTGGCCTTCGCGTCGGCGCGGCCGAGAGGCAAAGGGGAGGTCACTCAGCAGACCATCCAGAAG ATGCTGGATGAGAACCACCACCTGATCCAGTGCATCCTGGACTACCAGAGCAAGGGCAAGACCGCCGAGT GCACGCAGTACCAGCAGATCCTGCACCGGAACCTGGTCTACCTAGCCACCATAGCAGACTCCAATCAGAA CATGCAGTCCCTGCTTCCCGCGCCTCCAACACAGAACATGAACCTCGGGCCCGGAGCACTGAGTCAGAGT GGTTCCAGCCAGGGCCTGCACCCCCAGGGCAGCCTCAGCGATACCGTCAGCACAGGCCTGCCCCCCGCCT CCCTCATGCAGGGCCAGATCGGTAACGGTCCAAACCACGTGTCCATGCAGCAGACGGCTCAGAGCACACT GCCCACAACCTCCATGAGCTTGTCAGGCAGTGGCCATGGTACTGGCCCTGGGTACAGCCACTCGGGGCCT ACCTCGCAGAGTGTCCCCATGCAAGGCCAAGGTGCCATCAGCAACTATGTGTCTCGGACCAACATCAACA TGCAGTCTAACCCAGTCTCCATGATGCACCAGCAGGCAGCCACGTCCCACTACAACTCAGCACAGGGTGG AAGCCAGCATTACCAGGGCCAGGCACCCATTGCCATGATGGGCCAGGGTGGCCAAGGAGGCAGCATGATG GGGCAGCGGCCCATGGCGCCCTACAGACCCTCCCAGCAAGGCTCTTCCCAGCAGTACCTGGGCCAAGAGG AGTACTACAGCGAACAGTACAGCCACAGCCAGGGCTCCGCAGAGCCCATGAGTCAACAGTACTACCCGGA TGGCCACGGTGACTACGCCTATCAGCAGTCGTCCTACACAGAGCAGAGCTACGACCGCTCCTTTGAGGAT CCCACACAGCACTACTACGAGGGGGGAAACTCCCAGTACAGTCAGCAGCAGGCTGGGTACCAGCAGGGCA CAGCACAGCAGCAGACCTACTCCCAGCAACAGTATCCCAACCAGCAGAGCTACCCGGGGCAGCAGCAGGG CTATGGTCCTGCCCAGGGAGCCCCCTCACAGTACTCAAGCTACCAGCAAGGACAAGGTCAGCAGTATGGA AGCTACAGAACATCGCAGACGGGACCTTCTGCCCAGCAGCAGCGGCCTTACGGCTATGAACAGGGCCAGT ATGGAAATTACCAGCAATAAAGAACAAGCATTGTCTTTGGACCCTTCATAGTAGTATGTTCTGGACAAGC CGGTGGCAGTTCTGATGAGTAGCGACATGTTGGTCACCCTCTCTGCCCAGTGCCGTGTCTGCATGAGAGG CAGGCTGGTTTCATGCTGGGCGTGATGCTGTGTGCACCACTGACTGCGATATGGCGTGACATGTCTGGTG CTGTGTAAAGTATTGTATATCGGTACGATGGGGAGGTTGTCCTGTTTGTGTCCCCTGCCCGCTCCCTGAT GTTCTTAGCTAGCTTGGGGGGGGGTTACCGTGTCATCACATGTTCTGTGCCTGGTGATGAGACAATGTCT AAGAGACATCATGGTCCATGCTGCTGTGAACAGACTCAGTCTGCCCCCTCTCATACCATTGTTGCAAAGT GGACTGTAAATGTTTCTTCAACTGGCGGCTCATAGCTTGACATACATACACCGCTAGGTGACCATTTCTT CTGTGCCTAAGTAGGGCTTGAGGACACCTTCTGTGTCTTGGGTCATGTCACTATAACAAGGAAGATGTGC TTTGTGTGCAAGGACCATGAGCTGTCCTTTCCAGAACTTACCAATTGCCTGTGTCTCTCCAGTTTCCATG ATCCCAAAGGATGTCTTTGTATTAGCGAGTAAAGAAGGATCGATGACTATTCCAAATGACAGTCGGTGAG AGTGTGGGCATCGTGAGGGGAGCTTTCACTAAGGAGGGCGCTGTCTGAAGAGCAATTCTTGCTGTCTCCG AGCTGCTTGTGTGTAGTATGGCTTTGGCCCTTGGCGTCATCTCTAGGATTTGAGTGTGCCCAAACCTAAG TTTATAGAGAATTTAAAGTACTCATGTTTAATTTTACAAGCAGTTGGCAAGTGTTTATATTACTTCTTCA GGACCTCTGAGTTCAGATTGCCAAGCAGATGTTCCTTTGCGTTTAGGGAAGTCTGAAATCCACACTGCAT TTTTAATGAATCCAAATAACTTTATTACGTTCTTTCAAATGAAAAGGACAAGTTTTATACAGTGAGAATT GGGTGATTTTTTTTTTTTTTACATTCCTAGGATGTTTAATCTCGTTTTAATTCTGTGCAAACATGAGAGA CAGCCTTTTTGAAAAGGTTCTATCAAAGGAAAACACCGCACATAATGCAGCAGGCATATTTTTCAGCATT TATTTAGATAATAACAATGTTACTATGAGAAGAAAAGAACAACCTTGAAGATGAGTGCTCAGAACAACCA AGAACATACAAAACCATCCCCAAGGATGCAGGGCTTGGTACATCAGACCTGTCCACCAGGATGCTTTTGC TTTATTTGTGTCTTATATGTAGCCCAGACTGGCCTCAAACTCACTACATAGCTGAGGCTGAACTTAATGA TCCCCCAGCCTCCACTCCCGGGTACTGAGACCACGGGCATGCACCACCACATCTGTTTTCAGCTGATAGT TTTTAAAATATAAAACTTACCTGTAGCTCAGTAGTAAGGCACCTTTGTGGAGTTTGCTTGAATTAATCTT GACAGTCTTGCTGGAACTTACAGTGCAAGCTCCATTTACTTACAGGAGAATCAAGGGCCGGATCAAAGGA CTTGTCTGTGTCGGCTGCCGTCTTACTCTAGGACATTCTCCCTCTCCTTGGGACCATTATCAACAACCAT GGGCATATGTGTCTCATAGGCACAGGGTTTAGGAAACACACAGGCAAGGGCTGACTACATGACGGCTTTA ACTTTACAGAAACAAGTTTCTGATCGCTACATGGCAGAAGTATTAGCAACTTGATTTTAGGGACTCATCA TCTCTTTAGCTCCTTCCCTTTCTGGCAATTTTTATAAAACTAGCCTACAAGCTCACTTGGGGGCTAAATA TCCCATTGAAAATGTCGAAACATTTTAAGTAACTGTGAAATGGTTTTTATTCCTTGCCAATCTGGGAATA TGCCTTTTATGTATGCATACATGTGCAAGTGTGTACGTGTGTGTGTGTGTGTGTGTGTGTATGTATGTGT GTGTATGTGTGTATATATATGTGTGTATGTTAAAGTGCTGTATTAGTGTGTATGTGTGTGTGTATATGTA AAGTGCTGTGTTAGTGTGTGTTAATACTTTTTGAAAGAAAAGAACACTTAAAATATGTATCACCCCAAAA GTTCAATTTGAAATGTCTTACATTAAGAATTTCTTGAATGTTGTGTATATATTTTTAAAAGCACTTTGTG AAATAGTTTGTACATTTATTTCCTAATTTATACCTGATTTTGGTGTTAATATATTTAATGATTAATATTG TTTATTTAATGTTTTATTGATTTTTATGTAATTTTGTGGGGGAAAGTGATGCCAGTCTTTTTCATTGCCT GTATTATAGCTTTTCTTCTGTAACATGAATGTTTGAAAAGTCCTAGGCTGAAATGAACCCTTCGTGCAGG TGTGGTTGACTGTGTTTTGTTTTCAATGGTTCCTCCTACTGAAGGCCAGAAAAGATGCAAAGGGAGATTT GGAAATCGCTGCTCATTCTTCCTCCTGTTTCCCAGCATCCGTTCAATACTTGGTGAACTTGACCACTGAG GACTGGGGTTTTCAGATGTGCTGACCACTACGCGCTGCGCTTGTCAGGGGCTATGGGTGGATGGACATCT CTGCAGACTTAGCATATAGCACAGTGGGAGATGGGAGATGTCCGCAGAGGCACTGGGCAGACACAGGCCT GGCCCAGAAAGGAGGTGTTTTCCTTTCCTGTTGTACCTGTTCTCAGACGTGGGCTTCAGTGACTGAGTGT CCGTTCAAACTTGTAAAAAGAGGAAGACTTTCCATTTCCATTAAAACACTTTTTTAGCCATTAAAAAAAA AAAAAAAAAAAAA
• Restriction Sites: AscI-NotI
• ACCN: NM_178750
• Insert Size: 1209 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC053087, AAH53087
• RefSeq Size: 4213 bp
• RefSeq ORF: 1209 bp
• Locus ID: 269397
• UniProt ID: Q8BW22
• Cytogenetics: 2 H4
• Gene Summary: Transcriptional activator which is required for calcium-dependent dendritic growth and branching in cortical neurons. Recruits CREB-binding protein (CREBBP) to nuclear bodies. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP.[UniProtKB/Swiss-Prot Function]