Sema3e (NM_011348) Mouse Untagged Clone

CAT#: MC203244

Sema3e (untagged) - Mouse sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3E (Sema3e), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Sema3e
• Synonyms: 6430702L12; AA408817; mKIAA0331; Semah
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC057956
  GGCGGCATCCCCGCTGGGTGGATCCCGCGCTCCTGGCGCAAGTGGCACTTCTTGCTTCTAATTATCGAGA GGAGAGGCGAATACGAACTAGCTGCTCGGCAAGTCAGTGCAGGAGGCTGACTTCTGGGAGGCTGGCGGGG AGGCTGGGGGAAGAGCTGGGGGAGGCTGCTGCTCTGCTCCGACTGTTTTCTCAATGAATAGCTGGCGGGG AGACTGAAGCTAGCCACAGCCTCCTCCTCACTCCGCGTCTGGGTGACGGCGACAGCACCAGCCCGGACCT GGCTCTCAAGACGCGCTCCTTGGACGGTCTCTTGCTCCGCGCTTCTAACCACCGGGCCCAAGGACAGAAA GGCTTAGCGGATCCAAATATTGCCCGGCAAATGGCACTTGGGAATGGTATTTTCTGATGACAACCCCTTC TGTTTGTGACAAAGCCTGTCGCCCGCAGTTGCCCCTGGAGGGAAGTACTAAGTAAAACTCAATCCTGTCT TAAAGTGTGGCTGCAGGGGCCAGAGGAGAGCCAGCACGCACCATGGCACCGGCCGGACACATCCTCACCT TGCTGCTCTGGGGTCACCTGCTGGAACTCTGGACCCCAGGTCACTCCGCGAACCCCTCCTACCCCAGGCT ACGCCTGTCACATAAAGAACTTTTGGAACTGAATAGGACTTCAATATTTCAAAGCCCCCTTGGATTTCTT GATCTCCATACAATGCTGCTGGATGAGTATCAAGAACGGCTCTTTGTGGGAGGCAGAGACCTTGTCTATT CCCTGAACTTGGAACGAGTCAGTGACGGCTACAGAGAGATATACTGGCCGAGCACAGCAGTAAAGGTAGA AGAATGCATAATGAAAGGAAAAGACGCAAATGAGTGTGCCAATTATATCCGGGTTTTGCATCACTACAAC AGGACACACCTTCTGACCTGTGCTACTGGAGCTTTTGATCCACACTGTGCCTTCATCAGAGTCGGGCACC ATTCAGAGGAACCCCTGTTTCACCTGGAGTCACACAGATCTGAGAGAGGAAGGGGCAGATGTCCTTTTGA CCCCAACTCCTCCTTTGTGTCCACGCTAGTTGGGAATGAGCTGTTTGCTGGACTCTACAGTGACTATTGG GGCAGAGACTCGGCGATCTTCCGCAGCATGGGGAAGTTAGGCCATATTCGCACTGAGCATGACGATGAGC GGCTCCTGAAAGAACCAAAATTTGTAGGTTCATATATGATTCCTGATAACGAAGACCGAGATGACAACAA AATGTACTTTTTCTTTACTGAGAAGGCGCTGGAGGCGGAGAACAACGCCCACACGATCTACACCCGAGTG GGGCGGCTGTGCGTGAATGACATGGGAGGACAGAGAATCCTGGTGAACAAGTGGAGCACTTTCCTTAAAG CGCGGCTGGTTTGCTCAGTGCCGGGAATGAATGGAATCGACACATACTTTGACGAACTAGAGGATGTGTT TTTACTGCCGACCAGAGATCCTAAGAATCCAGTGATATTTGGACTGTTTAATACTACCAGCAATATATTT AGAGGCCATGCTGTATGTGTGTATCACATGTCAAGTATCCGGGAAGCCTTTAATGGCCCATATGCTCATA AAGAAGGCCCTGAATACCACTGGTCACTATATGAAGGAAAAGTCCCCTACCCAAGGCCTGGTTCCTGTGC CAGCAAAGTAAACGGAGGCAAGTATGGAACCACCAAAGATTACCCCGATGACGCCATCCGGTTCGCAAGG ATGCATCCTCTAATGTATCAGCCCATAAAACCTGTTCATAAAAAACCAATACTGGTAAAAACAGATGGAA AATACAACCTGAGGCAACTTGCCGTGGATCGGGTGGAAGCGGAGGATGGCCAGTATGACGTCTTATTTAT TGGGACAGACACAGGAATTGTGCTGAAAGTAATCACAATTTACAACCAAGAAACAGAGTGGATGGAGGAA GTCATTCTAGAGGAACTTCAAATATTCAAGGATCCAGCCCCTATCATTTCTATGGAAATTTCTTCAAAGA GACAACAGCTTTACATTGGATCAGCCTCTGCTGTGGCACAAGTCAGATTCCATCACTGCGACATGTATGG CAGTGCTTGTGCTGACTGCTGCCTGGCTCGAGACCCGTACTGTGCCTGGGATGGCATATCCTGCTCCAGG TACTACCCAACAGGTGCACACGCAAAGAGGAGGTTCCGCAGGCAGGACGTTCGGCATGGCAACGCCGCCC AACAGTGCTTTGGACAGCAATTTGTTGGAGACGCGTTGGACAGGACTGAAGAGAGGCTGGCTTATGGCAT AGAGAGCAACAGTACTCTGTTGGAATGCACCCCGCGATCACTACAAGCAAAAGTCATCTGGTTTGTACAG AAGGGACGCGACGTAAGAAAAGAAGAGGTGAAGACGGATGACAGAGTTGTCAAGATGGACTTGGGCTTGC TCTTCCTCAGAGTACGCAAGTCAGATGCAGGGACCTATTTTTGCCAGACAGTAGAACACAATTTTGTCCA TACTGTGCGTAAAATCACCTTGGAGGTGGTCGAAGAGCATAAAGTGGAGGGCATGTTTCATAAGGACCAT GAAGAGGAAAGACATCACAAGATGCCCTGCCCTCCCTTAAGCGGTATGTCTCAGGGGACAAAACCGTGGT ACAAGGAATTCTTGCAGCTGATTGGCTACAGCAACTTCCAGAGAGTGGAAGAATACTGCGAAAAGGTGTG GTGTACAGATAAGAAGAGGAAAAAGCTTAAAATGTCTCCCTCCAAGTGGAAGTATGCCAACCCCCAGGAA AAGAGGCTTCGCTCTAAAGCTGAGCACTTCCGCCTGCCCAGGCACACGCTGCTCTCCTGAGGGCGCCCTC TGCCGGCGGCTGAGGAACCTAGGATGGAAACATTTTTTTAAAGGGGGTTAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_011348
• Insert Size: 2328 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC057956, AAH57956
• RefSeq Size: 2935 bp
• RefSeq ORF: 2328 bp
• Locus ID: 20349
• UniProt ID: P70275
• Cytogenetics: 5 A1
• Gene Summary: Plays an important role in signaling via the cell surface receptor PLXND1. Mediates reorganization of the actin cytoskeleton, leading to the retraction of cell projections. Promotes focal adhesion disassembly and inhibits adhesion of endothelial cells to the extracellular matrix. Regulates angiogenesis, both during embryogenesis and after birth. Can down-regulate sprouting angiogenesis. Required for normal vascular patterning during embryogenesis. Plays an important role in ensuring the specificity of synapse formation.[UniProtKB/Swiss-Prot Function]