Lsm6 (NM_030145) Mouse Untagged Clone

CAT#: MC203203

Lsm6 (untagged) - Mouse LSM6 homolog, U6 small nuclear RNA associated (S. cerevisiae) (Lsm6), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Lsm6
• Synonyms: 1500031N17Rik; 2410088K19Rik; AI747288
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC030427
  CCACGCGTCCGGCGGAAGCCCGGCCTGTGAGCGTTCTGGATCCGGCTGCAAGGGTTGTTAAACATGAGCC TGCGGAAGCAAACCCCTAGCGACTTCTTAAAGCAGATCATCGGACGGCCAGTTGTGGTGAAATTAAATTC TGGCGTGGATTATCGAGGGGTCCTGGCCTGCCTGGATGGCTACATGAATATAGCCCTGGAACAGACAGAG GAGTATGTAAATGGACAGCTGAAGAATAAGTACGGAGATGCGTTCATCCGAGGAAACAATGTGCTGTACA TAAGTACACAGAAGAGGCGGATGTGAAGACACCAGGAGGCAGTGCTCTTCAGAGATGGATAGATGAAGTG GTTTTGGTTCTCTTACCATCTTAGTTGGCCTGTTTTCATGGTGGTTGGTGGGGGTTTGTTTGTTTGTTTG TTTGTTTAATTGACATTGGAATAAGATAGAGGCATTGCATTACAGATTGACTCTTAAGTTTTTCACATTT ATGTCTCAGTCTTTATCATTTCTGCCTGTACAGAATGCTAAAACTAAAACAGTTTGTTTCTCCAGGATTA TTTTACCTAAGATTCCTGCCCTGTCATTCCATTGGTTGATTACGTATTCAGCTGACTAAGTGGTACATCC CGGAACCTGTGATCAAGCATTCTGGAGGCATCCTTTGTCTCTTTCTCTACTCACCATCCGTACCATTTAA AGCAGGTAGATAGAAAGTCATATGGTTTCAGCCTTGGGCTATAAAGGGACAAGAGGTCAGAAAATCAGCT GTTTATTTAGTCACCTAGTTAGTGGTGGTATCTCCACAAAGGAGTGCAACTGTAGAGTCAGGAATTGGAA AGGTCAGCCACGTGAGGTGAGACACTGCCTGGAAGCCTCCTGTGCAGTTAGACTCTTCATGTTAACTTGC TCCCTGTTGAGAGTGTTGGTGCAATGGCTGGTTACGCCAGCCAGGGGAGGAGCTCTACTGTTCCTGAGAA ACTGGCTGTGGCTGAGGTGGTGCAAGCCCAGAGTGCTTAGACTCAGTTTCCAGGCACTGAGCTTCGGCTG CGAAAGTTTCTCCTGCTTTTTGCTTTAGCAAGTGGCCTTGGGGAAAGGGTTGTATTCTTGGCCTTCAGGT CACGTGACTCCCGCTGAGCGGGCTTGTGCTGTCTGTGGACTGTGTGGGTGCTTTGGTTGCATGGACGCTA GGGCTTTCCTTACCCTCCTTCAATCTAAACAGATCCCTGTCTCGGGAGGTCGGACAGTTTTAGAGCAATT GCCCGGCTGGAGGAAGTGGAGGCAGATGGACAGCTAAGGAGGGAGGGTGTGCTTTCTGGAGAGGTGTATA TATGGCATTTGTCTAATTTAAATAGTAATCAGAAACAATTAATAACTCAGGAAAGAGAGGAGTAAGGTTT GATGGTGTGCACACTGAAAATAAAACACTTTATCCTAAGCTTCAGTAATTCATCACTTCCTGACAGGGGC TTGCAAAGGCACCAGTGTGTAAGTACAAATCACCAATTTGCTCTTTTATAAATGGCTTCTTTAAAGGAGA AAATGGATGTCTCTCGGAGTGGAGCACACATGTTCTCATTTGTTTTTTAGAGTGTGTGAGGCCGCCAGGT TACTGCATCCCTTCATTCATCCCCTCAGCTGCCCTGAAAGCAGTCCTGCTGTTATCTCCAGCAGTCTGTA ACATCCCGGCTCTGCTCATTTCTTCCCTGACCTCCCTCCAGCCTGCAGGTCTTTATTTCCCTCTTGCCAT TTCTAGCTTAATAGCGCTACTGCAGAATATTGTTTTCATCCATGGAACCGCTTTTAATCAAGATGGACTT TGGGAAATGCTACCATTTTTATGGTAATAACCATATTATGCATCTGGACCAGCACATGTTGTCCCCTGTG CATACCTTCTTGTGATAGAATTCTGAAACAAATTGTAAATGGTAAAATTACTTTTTTTTTAAAAAAAAAA AAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_030145
• Insert Size: 243 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC030427, AAH30427
• RefSeq Size: 1965 bp
• RefSeq ORF: 243 bp
• Locus ID: 78651
• UniProt ID: P62313
• Cytogenetics: 8 C1
• Gene Summary: Plays role in pre-mRNA splicing as component of the U4/U6-U5 tri-snRNP complex that is involved in spliceosome assembly, and as component of the precatalytic spliceosome (spliceosome B complex). The heptameric LSM2-8 complex binds specifically to the 3'-terminal U-tract of U6 snRNA. Component of LSm protein complexes, which are involved in RNA processing and may function in a chaperone-like manner, facilitating the efficient association of RNA processing factors with their substrates. Component of the cytoplasmic LSM1-LSM7 complex, which is thought to be involved in mRNA degradation by activating the decapping step in the 5'-to-3' mRNA decay pathway.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks an exon in the 5' UTR compared to variant 1. Both variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.