Ell3 (NM_145973) Mouse Untagged Clone

CAT#: MC203102

Ell3 (untagged) - Mouse elongation factor RNA polymerase II-like 3 (Ell3), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ell3
• Synonyms: A930015D22Rik
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC045151
  GCCAGCCTAGGCTTGCACAGCAGAGGTGGCCCTCACCTGTGGTCCCGGTTCTGCATGTGCGCGACTTTGG CACCATGGAGGGGACCCAGGAAGCTCTGAGTGGGAAAATGCGGCTCCTCTTCACCCCCGCTGCTCGGACC AGCCTCCTGATGCTAAGGCTCAACGAGGCGGCGCTGCGGGCATTGCAAGAGTGTCAGCAGCAACAGGTAC GGCCAGTGATCGCTTTCCAAGGCCACCGAGGGTACCTAAGGTTTCCAGGCCCGGGATGGTCCTGCCTTTT CTCCTTCATAGTATCCCAGTGTGGCCAAGAGGGCACTAATGGTGGCTTGGACCTTGTGTACCAACGCTTA GGCAGATCTGGGCCGAACTGTCTCCACTGCCTGGGCTCCCTTAGAGAGCGACTCACTATTTGGGCAGCCA TGGATACTATCCCAGCTCCACTGTTAGCTCAGGAACACCTGACTGAAGGTACCAGAGAGTCTGAGAGCTG GCAGGACACTGGAGACGAACCTGAAGGCCATCCCCAGCTGGCACCAGATGAGGTGTCTGACCCACTGGCA AGCCACCATGAACAGTCACTCCCAGGATCCTCCAGTGAGCCCATGGCACAATGGGAAATGAGGAACCACA CTTATCTTCCAAGCAGAGAGCCTGATCAGTCCCTGCTTTCCCCTGCTAGCCAGAAACGCCTGGACAAGAA ACGTTCAGCACCTATAACCACTGAAGAACCAGAGGAAAAGAGGCTCAGAGCTCTGCCTCTAGCCTCAAGT CCACTACAAGGGCTAGCAAATCAGGACTCACAAGAGGGAGAAGACTGGGGCCAAGATGAAGATGAAGAGG GAGATGAAGATGGAGATTCCAGGCTTGAGCAGAGCCTCTCAGCTCCATCAGCCTCTGAATCCCCAAGCCC TGAGGAGGTACCAGATTATCTCCTGCAATACAGAGCCATCCACAGCACAGAGCAGCAACAGGCCTATGAG CAGGACTTTGAGACCGACTATGCTGAGTACCGCATTCTGCATGCTCGAGTTGGGGCTGCAAGCCAGAGGT TCACAGAGCTGGGGGCAGAGATCAAGAGACTTCAGCGAGGAACTCCAGAACACAAGGTGCTGGAAGACAA GATAGTCCAGGAGTATAAAAAGTTCAGAAAGCGGTATCCAAGTTACAGGGAGGAGAAGCATCGCTGTGAG TACCTGCATCAGAAACTGTCCCACATTAAAGGTCTCATCCTGGAATTTGAGGAGAAGAACAGGGGCAGCT GAGACTGTCCAGAGGGCTCGGATCTTCCCCGGTGATGAACACAACAGCTGCTTTTCTGATTTTGTCCCTT ACATCCACAGTGACCAAGTAGAGAGCCATCCTAGGTTCTTGCAGTTTGATTTTTGATGTTGCCATAATTT TTTTTAGGGTGTGGGCATGGTATCATCTCCTCAGCTGTGCCAGGAGACTAGGAAAGTAGGAGGTGCTTGG CTTCTTCAACTTTACTTTAGCTAAGTCATTTCTAGATCCTGAAAAGTGACATTTCCAGGTTAACCTTGAG AATATTAGACAAATAAGCAACTTGAGGGGAGTTGCCTTGCAGGGATAAAATGAGAACTTCTGATGACAGA ACTCATTGAACTTATAGTGTACCTTTATGTAACATGGCTGAAAAATTAAAACTGATTGCTTCTGAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_145973
• Insert Size: 1188 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC045151, AAH45151
• RefSeq Size: 1745 bp
• RefSeq ORF: 1188 bp
• Locus ID: 269344
• UniProt ID: Q80VR2
• Cytogenetics: 2 E5
• Gene Summary: Enhancer-binding elongation factor that specifically binds enhancers in embryonic stem cells (ES cells), marks them, and is required for their future activation during stem cell specification. Elongation factor component of the super elongation complex (SEC), a complex required to increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA. Component of the little elongation complex (LEC), a complex required to regulate small nuclear RNA (snRNA) gene transcription by RNA polymerase II and III. Does not only bind to enhancer regions of active genes, but also marks the enhancers that are in a poised or inactive state in ES cells and is required for establishing proper RNA polymerase II occupancy at developmentally regulated genes in a cohesin-dependent manner. Probably required for priming developmentally regulated genes for later recruitment of the super elongation complex (SEC), for transcriptional activation during differentiation. Required for recruitment of P-TEFb within SEC during differentiation. Probably preloaded on germ cell chromatin, suggesting that it may prime gene activation by marking enhancers as early as in the germ cells. Promoting epithelial-mesenchymal transition (EMT).[UniProtKB/Swiss-Prot Function]