Gfer (NM_023040) Mouse Untagged Clone

CAT#: MC203039

Gfer (untagged) - Mouse growth factor, erv1 (S. cerevisiae)-like (augmenter of liver regeneration) (Gfer), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Gfer
• Synonyms: Alr; ERV1
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC023941
  CCACGCGTCCGGCGCCGGCGGTGGCGTGCACGCTCCTCCGAACTGTCGCCGGCACGCCCTCTTGGCCCCG CTGCCCGCACGCCAGCAAGATGGCGGCGCCCAGCGAGCCGGCGGGCTTCCCTCGCGGCAGTCGCTTCTCC TTCCTGCCGGGCGGCGCGCGCTCCGAGATGACCGACGACCTGGTGACTGACGCGCGGGGCCGCGGCGCAA GGCATAGAGACGACACCACCCCTTCCGCGGCCCCGGCGCCGCAAGGTTTGGAGCACGGGAAGCGACCGTG CCGGGCCTGCGTGGACTTCAAGTCGTGGATGCGGACCCAGCAGAAGCGGGACATCAAGTTTAGGGAGGAC TGTCCGCAGGATCGGGAAGAATTGGGTCGCCACACCTGGGCTTTCCTCCATACGCTGGCCGCCTATTACC CGGACAGGCCCACGCCAGAACAACAACAGGATATGGCCCAGTTCATACATATATTTTCCAAGTTTTACCC CTGCGAGGAATGTGCGGAAGACATAAGGAAGAGGATAGGCAGGAACCAGCCAGACACAAGCACTCGAGTA TCCTTCAGCCAGTGGCTGTGCCGCCTGCACAATGAGGTGAATCGGAAGCTGGGCAAGCCTGATTTTGACT GCTCGAGAGTAGATGAGCGTTGGCGTGACGGCTGGAAGGACGGCTCCTGTGACTAGAAGATTACCAGCAG TTCGTGAGGGGCATCTAGCCTGGTTCTATGGGCAACAGCCTGATTGACGATTAAAGTGCATCTGAGCCAA CACTTGTTTCTGTAGTTGGGGGAGAAATCCCTAGAACACTGCCTCTGGGAACCCTGCCACAGGATTAAGA AATGGAGGTGCCTACTACAGACACTTGGGTGGCCTCCTCAGCGAAAGCCCCATGAGACTGAAGGTCAGAG GCAGGAGTGGTCCAGGTGCCTCGTACCCTTCATTTGGGACATAGTTGCCCCAAAGGAAGATGGGCTAGAT GATTGCCTTACTACTACACAGCCCTGGCTGCCTACAAGAACTGTGATCCTGGCAACTCTGCCATTTCAGA ATTGAAGCAGGGTTCCCCAGCTCCAGCAGGGTTTTTCTCTTAGGGTGTACCAACCATGGTGCCCACTGTC AGCCTGGCACATGGTCTTCTGCAGCTATGACAAGCATGTCAGCAGAGGGTCCTGGGAACAGCTTCCTTAG CGTTTGAGGCCAAAATAAACTGAAGTGACTTAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_023040
• Insert Size: 597 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC023941, AAH23941
• RefSeq Size: 1238 bp
• RefSeq ORF: 597 bp
• Locus ID: 11692
• UniProt ID: P56213
• Cytogenetics: 17 12.48 cM
• Gene Summary: FAD-dependent sulfhydryl oxidase that regenerates the redox-active disulfide bonds in CHCHD4/MIA40, a chaperone essential for disulfide bond formation and protein folding in the mitochondrial intermembrane space. The reduced form of CHCHD4/MIA40 forms a transient intermolecular disulfide bridge with GFER/ERV1, resulting in regeneration of the essential disulfide bonds in CHCHD4/MIA40, while GFER/ERV1 becomes re-oxidized by donating electrons to cytochrome c or molecular oxygen (By similarity).[UniProtKB/Swiss-Prot Function]