Agk (NM_023538) Mouse Untagged Clone

CAT#: MC202948

Agk (untagged) - Mouse acylglycerol kinase (Agk), nuclear gene encoding mitochondrial protein, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Agk
• Synonyms: 2610037M15Rik; 6720408I04Rik; AI465370; Mulk
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC019145 sequence for NM_023538
  CCACGCGTCCGACACAGGAAAGGCCGGGCGCTGAGCGGCGGGCAGCGGAGAAGATGACTGCATTCTTTAA AACGCTTCGAAATCATTGGAAGAAAACTACAGCTGGGCTCTGTTTGCTGACATGGGGAGGTCACTGGCTC TACGGGAAGCACTGTGATAACCTGCTAAGAAGAGCAGCCTGTCAAGAAGCTCAGGTGTTTGGCAACCAGC TCATTCCTCCCAATGCACAAGTGAAGAAAGCCACCGTCTTTCTCAATCCCGCAGCTTGCAAAGGCAAAGC CAGAACTCTATTTGAAAAAAATGCTGCTCCAATTTTACACCTGTCTGGCATGGATGTGACTGTTGTCAAG ACGGATTATGAGGGACAAGCCAAGAAGCTCTTGGAGTTGATGGAAAGCACTGATGTGATCATTGTTGCTG GAGGAGACGGCACACTGCAGGAGGTTGTTACTGGAGTACTGAGAAGAACAGATGAGGCTACCTTCAGTAA GATTCCCATTGGATTTATCCCGCTGGGACAGACCAGTAGTTTGAGTCACACTCTCTTTGCTGAAAGTGGA AACAAAGTCCAACATATTACAGACGCCACACTTGCCATTGTGAAAGGAGAGACTGTTCCACTTGATGTCT TGCAGATCAAGGGTGAAAAAGAACAACCTGTGTATGCAATGACTGGCCTCCGGTGGGGATCTTTCAGAGA TGCTGGCGTCAAAGTTAGCAAGTACTGGTATCTTGGTCCACTAAAAACCAAAGCAGCCCACTTTTTCAGC ACTCTTCAGGAATGGCCTCAGACTCATCAAGCCTCCATCTCTTACACGGGACCTAGAGAGAGACCTCCCA TTGAGCCTGAAGAGACTCCTCCCAGGCCGTCTCTGTATAGGAGAATATTACGCAGGCTTGCCTCATTCTG GGCACAGCCACAGGATGCCTCCTCCCGAGAGGTAAGCCCTGAGGTCTGGAAAGATGTACAACTGTCCACC ATTGAACTGTCCATCACAACCCGAAACACCCAACTTGACCTGATGAGCAAAGAGGATTTTATGAACATTT GCATTGAACCTGACACTGTCAGCAAAGGAGATTTCATAATCATAGGAAGTAAGAAGGTGAGAGACCCTGG CCTGCGTGCAGCTGGCACCGAGTGCCTCCAAGCCAGCCACTGTACTCTGGTTCTTCCTGAGGGAACTGAG GGCTCCTTCAGCATTGACAGCGAGGAGTACGAAGCCATGCCGGTGGAGGTCAAACTACTGCCCAGAAAGC TGCGGTTCTTCTGTGATCCGAGGAAGAGAGAGCAAATGCTGCCGAGCACATCCCAGTGAGCGACAGCGTG GCGGGCACTCCCAGGCTGCACTGCAAGCCACCAGTGGGACCACATGGGGGATGTGTGTGCTTCTGTCCCC AGAGGGTCACAGGATCCCAAGGGAATTTTCATGGCAAGTACCCCCAGCCCTCCTGTTTGCTTCCTAGAGC ACATACCATGTCATGCACTTGTTTGTGGTCAGTACCCTGTTAACCCTCGCTTTCCTTATGTTGAGGTAGT TGATCCCTCTTACACAGTGGTTGTCTCAGGCTGGGTCTAGAACGTTCTTGTACTCCCTGAGTTGGGAAGG GCTGGAGTTTTGTGATTGTCCCACAGTGGAGAGTGGAATCTGTAGACCAAGTTCCTGTTTCGGTTCTCTG ACCGTCCTGGGGCACCCCGGGGAGGCAGCTGCTGTCTCCGGGAGCCTCGCCCTCTTTCTGGGCTCTCTCA TGGGTGCTGCTCCTTTGTGAGCTCTCTGCTTTGGTTGGTTGGTCTCATCCGGAAAGCAAATGTGAGGTTG TTCCCTCTTTAGCCATGGCAGACGTATTGTAAAATGCTATTTTAAAACAAACTAGTGCTTCCTCATTGTT AGTTTGCATGTGTACAACTGAGGCGCATGTTGAAAACTAACTGACCTGGGACCTACCCCCAGGTTGACTT TGTAACTCAACCTGGCAGGAAGCTAGGGTTCTTCATTTTTAAAAAACTGCTTCCCCAGGTGAATCAACCA TACCGGGAGAAAGTGTTTTAAATCTACTTCATAAACTTAAATGTATTATATATATATATTTTTTAAAATC ACATATTTGTGTTGACATCTATTGTTTCCATGGGAAGTTAAACACTTGTCAGTGTACTTACTAGTATTGT GTAAATATTGTTTAAAAATAAAAACTGTTTGATCATTCTTTTAAACATAAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_023538
• Insert Size: 1266 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC019145, AAH19145
• RefSeq Size: 2236 bp
• RefSeq ORF: 1266 bp
• Locus ID: 69923
• UniProt ID: Q9ESW4
• Cytogenetics: 6 B1
• Gene Summary: Lipid kinase that can phosphorylate both monoacylglycerol and diacylglycerol to form lysophosphatidic acid (LPA) and phosphatidic acid (PA), respectively (PubMed:15252046). Does not phosphorylate sphingosine (PubMed:15252046). Independently of its lipid kinase activity, acts as a component of the TIM22 complex (By similarity). The TIM22 complex mediates the import and insertion of multi-pass transmembrane proteins into the mitochondrial inner membrane by forming a twin-pore translocase that uses the membrane potential as the external driving force (By similarity). In the TIM22 complex, required for the import of a subset of metabolite carriers into mitochondria, such as ANT1/SLC25A4 and SLC25A24, while it is not required for the import of TIMM23 (By similarity). Overexpression increases the formation and secretion of LPA, resulting in transactivation of EGFR and activation of the downstream MAPK signaling pathway, leading to increased cell growth (By similarity).[UniProtKB/Swiss-Prot Function]