Adgrg1 (NM_018882) Mouse Untagged Clone

CAT#: MC202826

Gpr56 (untagged) - Mouse G protein-coupled receptor 56 (Gpr56), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Adgrg1
• Synonyms: Cyt28; Gpr56; TM7LN4; TM7XN1
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC034678 sequence for NM_018882
  CCTGAAAGGTGGTTCAGCTGCCTGGCTCCTTCCAGAACCCGTCGCCTGGCACCAGGTAGGAGTATAATGG CTGTCCAGGTGCTGCGGCAGATGGTCTACTTCCTACTGAGTCTGTTTTCTCTGGTGCAAGGTGCACACAG TGGCAGCCCCCGAGAAGACTTCCGCTTCTGTGGCCAGCGGAACCAGACCCAACAGAGCACCCTCCACTAT GATCAATCTTCAGAGCCTCACATCTTTGTGTGGAACACAGAGGAGACCCTCACAATTCGTGCCCCCTTCC TGGCAGCCCCAGATATTCCCCGCTTCTTCCCAGAGCCTAGAGGGCTCTATCACTTCTGCCTCTACTGGAG TCGCCACACTGGGAGACTCCACTTGCGCTATGGCAAGCATGACTACCTGCTTAGTAGCCAAGCCTCCAGA CTCCTCTGCTTCCAGAAACAGGAGCAGAGCCTGAAGCAGGGAGCCCCGCTGATCGCCACCTCTGTCAGCT CCTGGCAGATTCCCCAGAACACCAGCCTGCCTGGGGCTCCGAGCTTCATCTTCTCCTTCCACAATGCCCC ACACAAGGTCTCCCACAATGCATCTGTGGACATGTGTGATCTCAAGAAGGAATTGCAGCAGCTTAGCAGG TACCTGCAGCACCCTCAAAAGGCTGCCAAGCGGCCCACCGCAGCGTTCATCAGCCAGCAGTTACAGAGCC TGGAGTCAAAGCTGACCTCTGTGAGCTTCCTGGGAGACACATTATCCTTTGAGGAGGACCGGGTCAATGC TACAGTGTGGAAGCTGCCACCCACAGCCGGTCTAGAGGATCTGCATATCCACTCCCAGAAGGAGGAGGAG CAGAGTGAGGTCCAGGCATACTCGCTGTTGCTTCCCCGGGCCGTATTCCAGCAGACCAGAGGCCGTCGCC GGGATGACGCCAAGAGGCTCCTGGTAGTAGACTTCAGCAGCCAAGCTTTGTTCCAGGACAAGAATTCTAG CCAAGTCCTGGGTGAGAAGGTCTTGGGTATTGTCGTGCAGAACACCAAAGTCACCAACCTCTCAGATCCG GTGGTACTCACCTTCCAGCACCAGCCTCAGCCAAAAAATGTGACTCTGCAGTGCGTGTTCTGGGTTGAAG ACCCGGCATCAAGCAGCACAGGGAGCTGGAGCAGTGCAGGCTGCGAGACAGTGAGCAGAGACACACAGAC ATCCTGCCTGTGCAACCACCTGACCTACTTTGCAGTGCTGATGGTGTCATCCACAGAGGTAGAAGCCACT CACAAACACTACCTCACGCTCCTGTCCTACGTGGGCTGTGTCATCTCTGCTCTGGCTTGTGTCTTCACTA TTGCTGCCTACCTCTGCTCCAGGAGGAAGTCACGTGACTACACCATCAAAGTCCACATGAACCTGCTGTC CGCTGTCTTCCTGCTGGACGTGAGCTTCCTGCTCAGCGAGCCTGTGGCACTGACGGGCTCCGAAGCAGCC TGTCGCACCAGTGCCATGTTCCTGCACTTCTCCCTGCTTGCCTGCCTCTCCTGGATGGGCCTCGAGGGCT ACAATCTCTACCGACTGGTGGTGGAGGTCTTCGGTACCTATGTGCCCGGCTATCTGCTCAAGCTGAGCAT CGTGGGCTGGGGTTTTCCTGTCTTCCTGGTCACTCTGGTGGCGTTGGTGGATGTGAATAACTACGGCCCC ATTATCCTAGCTGTGCGCCGGACTCCGGAACGTGTCACCTACCCCTCTATGTGCTGGATCCGGGACTCCC TGGTGAGCTATGTCACCAACCTGGGCCTCTTCAGTCTGGTGTTCCTGTTCAACCTGGCTATGCTGGCCAC CATGGTGGTGCAGATCCTGCGGCTTCGCCCGCACAGCCAGAACTGGCCCCACGTGCTGACCCTGCTGGGC CTCAGCCTGGTCCTTGGCCTCCCCTGGGCCTTGGTCTTCTTTTCCTTTGCTTCCGGCACCTTCCAGCTTG TCATCCTCTACCTCTTCAGCATCATAACTTCCTTCCAAGGCTTCCTCATCTTCCTGTGGTACTGGTCCAT GCGGTTCCAGGCCCAAGGCGGCCCCTCCCCTCTGAAGAACAACTCAGACAGCGCCAAACTCCCCATCAGC TCCGGCAGCACCTCCTCCAGCCGCATCTAAGCCACCGCCACACCTCCCCTCCGGGAGGACACATGCATGG CGTCCGCTCACGATGTCTGTGGCCCAGTGCTGTGCCCACCCAGCCTTTGTTGGTTAGTGGCATACTAGAG AAGGCCCTGGTCCTTGAAGGCGTAGGGCTGTTGCTCTGGTAGGTAGATACCTAGCTTGCCTTGGGGACGA CTCTGGTCCTCAAAGGGCTCAGAAGCACACTGCCATTCTGTTTGTGGGGCCGTTTCAGTCTGGAGCTAAG GCCTTGTCTTTCTGGCCACCTCTGGGTCCAGCTGTTGCTGCTGGGTGTTGAGACCTGCAGACCCAAGCTG GGGTTAGATCTCGAAGGAGGCTGACACATCCGGCCTGAGACACAGCTAACTGTCTTGACTTGCTGCTCTG TCTCTGTGGTCACCATGCAGATCCCGAGGGTGGCACTGGGGGTAAATGTTCTGGGAGAAGGTTTGGAGGC AGAGCACCTTAGGAGCTGAGCATCTCCCCCAGCCTTTCTGCAAACCCTCCTCTTCATTCCCCATCCCCAA CCCCTCCTCTGTGTTCCCCTAACCCTCCACCTGAAGCCTGGGGTCCTAGACCAATGCTGTGATTTGGGGT GGTAGTTCCCAGCAGTTTCCTGGTGCCAGCTATCAACTTCTGTCTGTTGTGTGGGCTTTGGCCTCTGACT CAGGGCAGGTTTCTGTCTGAGCCCTCTCTCCAAGCTGCCTCACCTTTGCTCGCACCTCAGAGGGACCTCC ATCTCTCCTGAAGCCTCCTCCCTCTGGCAAGTACTGGGATACAGCCACCCTTTCAACCCAGCACTCTGAA GACCAAGACAGCCCCCTCTGGTGACACTGGCCAAGCTTGATCTTTTTCCTAAGAAGTGGTCTTCAGATCC CTGCAGGTCGCTCAGAAGACACTGGGCTGCCTAGTGTGAATTCTGTCCTACTAACGTACAGTGAGCAGCT CCTCACCCCCACCCCCGCAAAAGCTCTCACCAAGTCCTGGAGTGTCAGGCAGGGGGCTGGAAATCCAGGA GGACTTCCTGCAAAAGGCAGCATTTCATCTTGACCTCAGCCTTCAGGTTGGGGAGAATGTTCTTTTTAAA TACCAGTTCATTTGTCTTTTGATATTAAAGCTCTTTATAGAGAGTCTGGAAACTGTAGGCGATTGTCGAG AAGAGAAATAAAAATGAGCTGTTATCTAATGCCATGGCAAAGCAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_018882
• Insert Size: 2064 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC034678, AAH34678
• RefSeq Size: 3348 bp
• RefSeq ORF: 2064 bp
• Locus ID: 14766
• UniProt ID: Q8K209
• Cytogenetics: 8 47.12 cM
• Gene Summary: Receptor involved in cell adhesion and probably in cell-cell interactions. Mediates cell matrix adhesion in developing neurons and hematopoietic stem cells. Receptor for collagen III/COL3A1 in the developing brain and involved in regulation of cortical development, specifically in maintenance of the pial basement membrane integrity and in cortical lamination (PubMed:21768377). Binding to the COL3A1 ligand inhibits neuronal migration and activates the RhoA pathway by coupling to GNA13 and possibly GNA12 (By similarity). Plays a role in the maintenance of hematopoietic stem cells and/or leukemia stem cells in bone marrow niche (PubMed:23478665). Plays a critical role in tumourigenesis (By similarity). Plays essential role in testis development (PubMed:20981830).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Variants 1 and 2 encode the same protein.