Bap1 (NM_027088) Mouse Untagged Clone

CAT#: MC202559

Bap1 (untagged) - Mouse Brca1 associated protein 1 (Bap1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Bap1
• Synonyms: 2300006C11Rik; AA989761; AW553466; mKIAA0272; uch-x4
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC050901 sequence for NM_027088
  AGAAGCCCTAAGGGCGGTGGGCGCTCCCGGTTGGGGGCGGCGCGGGTTTAGCAGGGCCTGGACATGGCGC TGAGAGGCCGCTCCGCGGGAAGATGAATAAGGGCTGGCTGGAGCTGGAGAGTGACCCAGGCCTCTTCACC CTCCTGGTGGAAGATTTCGGTGTCAAAGGGGTGCAAGTGGAGGAGATCTATGACCTTCAGAGTAAATGCC AGGGGCCTGTGTATGGATTTATCTTCCTGTTCAAATGGATCGAAGAGCGCAGGTCCCGCCGCAAGGTTTC TACGTTGGTGGATGACACGTCTGTGATTGATGATGATATTGTGAATAACATGTTCTTTGCTCACCAGCTG ATTCCCAACTCTTGTGCCACTCACGCCTTGCTAAGCGTGCTTCTGAACTGCAGCAATGTGGATCTGGGGC CCACGCTGAGCCGAATGAAGGATTTCACCAAAGGCTTCAGTCCTGAGAGCAAAGGATATGCAATTGGCAA TGCCCCTGAACTGGCCAAGGCACATAATAGCCATGCCAGGCCAGAACCACGTCACCTTCCTGAGAAACAG AATGGCCTCAGTGCAGTGCGGACCATGGAGGCGTTTCATTTTGTCAGCTATGTGCCTATCACAGGGAGGC TCTTTGAATTGGATGGATTGAAAGTCTACCCAATTGATCATGGGCCCTGGGGAGAGGACGAGGAGTGGAC AGATAAAGCTCGAAGGGTCATCATGGAGCGAATCGGTCTTGCTACTGCAGGGGAGCCCTATCATGACATT CGCTTCAACCTGATGGCAGTGGTGCCTGACCGCAGGATTAAGTATGAGACCAGGCTACATGTACTGAAGG TGAACCGACAAACAGTCCTGGAGGCCCTGCAGCAGCTGATTAGAGTAACCCAGCCGGAGCTGATTCAGAC CCACAAATCTCAAGAGTCACAGCTGCCTGAGGAGAGCAAGCCAGCCAGCAGCAAGTCCCCCCTTGGACTG GAGGCAGGCAGAACCCCAGTGGCCTCTGAGTGCACTCAGACAGATGGTGCAGAGGAGGTGGCTGGTTCGT GCCCACAAACTACAACCCATAGTCCTCCCAGCAAATGTAAGCTGGTGGTGAAGCCTCCAGGGAGCAGCCT CAATGGGGTTCCCCCAAACCCTGCCCCTATTGTCCAGCGGCTGCCAGCCTTCCTAGACAATCACAATTAT GCCAAATCCCCTATGCAGGAGGAGGAAGACCTGGCGGCAGGAGTGGGTCGCAGCCGCGTTCCCGTCCGAG CGCCCCAGCAGTACTCTGAAGACGAGGATGACTACGAGGATGAGGACGAGGACGTGCAGAACACCAACCC TGCCATCAGATACAAGCGGAAGGGGACAGGGAAGCCAGGATCGTTGAGCAATTCTTCAGATGGGCAGCTG TCAGTGCTGCAGCCCAACACCATCAATGTCTTAACTGAGAAGCTTCAAGAGTCTCAGAAAGACCTTTCAG TTCCTCTGTCCATCAAGACTAGCAGTGGGGCTGGGAGTCCAGCTGTGGCTGTGCCCACACACTCGCAACC TTCACCCACCCCTAGCAATGAGAGCACGGACACAGCCTCTGAGATTGGCAGTGCTTTCAACTCACCCTTG CGCTCGCCCATCCGCTCGGCCAACCCAACACGGCCCTCTAGCCCTGTCACCTCTCACATCTCCAAGGTGC TTTTTGGAGAAGATGACAGCCTACTTCGTGTTGACTGCATACGCTACAACCGTGCTGTCCGTGACCTGGG TCCTGTCATTAGCACGGGCCTGCTGCACCTTGCTGAAGATGGTGTACTGAGTCCCCTGGCACTCACAGAG GGTGGGAAGGGTTCCTCACCTTCTACCAGATCAAGCCAAGGCAGCCAGGGGTCCAGTGGCCTAGAGGAGA AGGAGGTGGTGGAAGTCACAGAGAGCAGAGACAAGCCTGGGCTGAACAGGTCCAGTGAGCCCTTGAGTGG AGAGAAGTACTCACCCAAGGAGCTGCTAGCACTGCTAAAGTGTGTAGAAGCCGAGATTGCAAACTATGAG GCCTGTCTCAAGGAGGAGGTGGAGAAAAGGAAGAAGTTCAAGATTGATGACCAGCGAAGGACCCACAACT ATGACGAGTTTATCTGTACCTTCATATCCATGCTGGCTCAGGAAGGAATGCTGGCCAACCTAGTGGAACA GAACATCTCAGTGCGCCGGCGCCAAGGGGTCAGCATTGGGCGGCTTCACAAGCAGCGGAAGCCTGACCGG CGGAAACGCTCTCGCCCCTACAAGGCCAAACGCCAGTGAGAACCTCTGGCCCTGACTCTGCAGCCTCTCT TGCCGCAAGGCCCTCATCAGGGCCCTTCACTGTCCCTCTTTTCCAAGTATTACTGAGTAGTTCAACGAGA GCCCAGGTCCTGGGAGTGGGAGTGACATGTATTCTGCACTGTGCTCTGGGGTCTGGCAGGGACAAGGCAG CTCCTCAATGCTCAGGAAGCAGCAGCTGGAACTGGAGGGCATCCACCATGGCCTCCACAGCCATCTGAGG AGCAGCAGGACCTGGCCTTTCTGCCTGGGCAGCAGGATGTACATTCTACCTATTGGAGATGTTTAGGTTT TGGGCTTTTGTCCATCTTGCCACTGTATTAACATGGCAGCTTCCTGACTCTGCTGTCTCTCCCAGCAGCT GTCATTCTGCTGGGCCAGGTCTCTTAGAATACATCACAGTCCCAGCTGAGAAACACACCACCACTAGCCT GTCCTCGTTGGGAAGAAGCTTGGCCTACACTTCATTCCTCTGGGAGAGCACCAAACTCAGGGACCTGGCT GCTGAGCTGGAATGGGCATGGGGTATCCTAATAGGATGTACAGCCTATCAGGGTCCCACGGCTATAGTGG AGACAAGTGTTTGAACTGTCCCAGTGGCATGCAGACTGTCCTGTTAGTGAGTCAGAGCAAGGCTCTGGGG CTCTTTGCCTTCAGTGTTGTGGCCCTGGCCATGGGTCTGCCTTAGGCTCCTAATCTCTGTCTCTGGGGCC CAGGGGAGCCTCGACCTAGCCCCTCAGTATTACCATGTCTTCTCTTAGGAATATCAGAGGCAGGGCTGCT TGAATGGAGCTGGTACTATTCAATTCTCCCGACTGTGCCAGCTTCCTTGTCCTCTTGCAAGGTACTCAGG GTAGGGACAGGGTTGAGAGGAGTTAGAACTTCTGTATCCCCATAGGGCTTGGTAGTAAGTACAGCACTGC TTCAAAATCCAGCCCCCAAAAGCCCTGTACCCTCTGCTAGATACAGGCGATCCAAGCAGGCCCCTTATCT GTACATAGTGACTGTAGGGGACCCCTCTAGTTTCTGTAAATATTGAATCAGTGAGTGAATAAAAATGTGC TAATAAATGAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: AscI-NotI
• ACCN: NM_027088
• Insert Size: 2187 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC050901, AAH50901
• RefSeq Size: 3390 bp
• RefSeq ORF: 2187 bp
• Locus ID: 104416
• UniProt ID: Q99PU7
• Cytogenetics: 14 B
• Gene Summary: Deubiquitinating enzyme that plays a key role in chromatin by mediating deubiquitination of histone H2A and HCFC1. Catalytic component of the PR-DUB complex, a complex that specifically mediates deubiquitination of histone H2A monoubiquitinated at 'Lys-119' (H2AK119ub1). Does not deubiquitinate monoubiquitinated histone H2B. Acts as a regulator of cell growth by mediating deubiquitination of HCFC1 N-terminal and C-terminal chains, with some specificity toward 'Lys-48'-linked polyubiquitin chains compared to 'Lys-63'-linked polyubiquitin chains. Deubiquitination of HCFC1 does not lead to increase stability of HCFC1. Interferes with the BRCA1 and BARD1 heterodimer activity by inhibiting their ability to mediate ubiquitination and autoubiquitination. It however does not mediate deubiquitination of BRCA1 and BARD1. Able to mediate autodeubiquitination via intramolecular interactions to couteract monoubiquitination at the nuclear localization signal (NLS), thereby protecting it from cytoplasmic sequestration. Acts as a tumor suppressor.[UniProtKB/Swiss-Prot Function]