Vps33a (NM_029929) Mouse Untagged Clone

CAT#: MC202323

Vps33a (untagged) - Mouse vacuolar protein sorting 33A (yeast) (Vps33a), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Vps33a
• Synonyms: 3830421M04Rik; AI503300; AW048546; AW554476; bf
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC046417 sequence for NM_029929
  GGGGAAATGCGGGTGCCCGGGCAAGATGGCGGCGCACCTGTCGTACGGGCGAGTGAACCTGAACGTGCTG CGCGAGGCGGTGCGTCGGGAGCTGCGCGAGTTCCTGGACAAGTGCGCTGGGAGCAAGGCAATAGTGTGGG ATGAGTACTTAACAGGACCATTTGGACTGATCGCACAGTACTCACTACTAAAGGAACATGAAGTGGAAAA AATGTTCACACTTAAAGGAAGTCGTCTGCCCGCAGCTGATGTCAAGAACATCATTTTTTTGGTCAGACCC AGGCTAGAGCTGATGGACATAATTGCTGAGAATGTACTCAGTGAGGACAGGCGTGGCCCTACCAGGGATT TCCATATTTTGTTTGTGCCTCGCCGGAGCCTACTGTGTGAGCAGCGGCTGAAGGACCTGGGGGTTTTGGG ATCCTTTATTCATCGAGAAGAATACAGCTTGGATCTCATTCCATTTGATGGGGACCTCTTATCCATGGAG TCAGAGGGCGCTTTCAAAGAGTGCTACCTGGAAGGCGACCAGACCAGCCTGTACCACGCAGCCAAGGGGC TGATGACCCTGCAGGCGCTGTATGGAACTATCCCCCAGATCTTTGGGAAAGGAGAGTGTGCTCGGCAAGT GGCCAATATGATGGTCAGGATGAAGAGGGAGTTCACAGGAAGCCAGAACTCAGTGTTCCCCGTGTTTGAT AACCTCTTGCTGCTTGATCGCAATGTGGATCTCCTGACGCCACTCGCCTCCCAGCTCACGTATGAAGGCC TCATCGATGAGATTTACGGCATTCAGAACAGTTATGTGAAGTTGCCTCCAGAGAAGTTTGCACCCAAGAA GCAGGGTGGCGGCAGTGGTGGGAAGGACCTTCCCACGGAGGCCAAGAAGCTTCAGCTCAACTCCGCAGAG GAGCTCTACGCCGAGATCCGAGACAAGAACTTCAACGCTGTGGGCTCTGTGCTCAGCAAGAAGGCGAAGA TCATCTCTGCAGCGTTCGAGGAGCGACACAACGCTAAGACAGTCGGGGAGATCAAGCAGTTTGCGTCCCA GCTACCCCACATGCAGGCAGCCAGGGGATCGCTCGCCAACCACACCTCAATTGCAGAGTTAATCAAAGAC GTCACCACTTCTGAAGACTTCTTTGATAAGTTGACAGTGGAGCAGGAGTTCATGTCCGGGATAGACACTG ATAAGGTCAACAGTTACATCGAGGACTGCATTGCCCAAAAGCATCCACTCATCAAGGTGCTGAGACTGGT TTGCCTCCAGTCTGTGTGCAACAGCGGGCTCAAGCAGAAGGTGCTGGACTATTACAGAAGGGAGATCCTC CAGACCTACGGCTATGAGCACATACTGACCTTGAACAACCTGGAGAAGGCCGGCCTGCTCAAGGCTCAGA CAGGGGGCAGAAACAATTACCCAACAATCCGGAAAACACTGCGCCTCTGGATGGACGATGTCAATGAACA AAACCCCACAGACATATCCTATGTGTACAGCGGTTATGCTCCACTCAGCGTGCGACTGGCTCAGCTCCTT TCCCGGCCAGGCTGGCGGAGCATCGAGGAGGTTCTCCGCATTCTCCCAGGGCCCCACTTTGAAGAAAGAC AGCCACTGCCCACCGGGCTGCAGAAGAAACGCCAGCCAGGAGAAAACAGAGTCACACTGGTATTTTTCCT CGGGGGCGTCACCTTTGCGGAAATAGCCGCCCTGCGATTTTTGTCCCAGCTGGAAGATGGAGGCACCGAG TATGTCATTGCGACCACTAAACTGATGAATGGAAACAGCTGGATAGAAGCTCTCATGGAAAAGCCTTTCT AGAGTATCGGAGGAGACGTGACAGGTCACCACACAATGAGCTGCCTCTGTGAACGTGTTGCCAAAGGATG TAAGGCCAGGAACAGGAGGACGGAAGCAGTGGGCGCATGTTCCTTCCTGCTCCTCTCTATGCTCTTAATC CTAAAGAAGGGACAAGCAACCTGGATGAAGGGATGATGAACTGGGCATAAGAGACAGAGGCAGGAGGATT GCTGCAAGCTTGAGGACAACCTGGGCTCCATAATGAGTTTCAGGCCAAAGCTCTCCCTTCCAGAAATAAA GGAGATCCTGTTTTGGAATTTTTAAGTCTCAAGTCTTCATTTTCTGGAAATGGCTAAGACTATGGGTCTG ATAGCCCTGGCAACCACATGACTGCACAGACATTAAAAGCAGAGCTGAGGGGACGAATTCAAAAAGAGAA TCAGAATTCTGACCACTGATCCACAGTGGCATATTCTAGAAGATGTCCCATGCCCAGGACTGTGGTTTTC TGTTGAATTGGAAGCCAGCATTGTCCAGTGTGTGCACTTTCCTGCAAAGCACATTTGTGTCTCCAGTCTC TACCGAGTTATTCTTAAGGCGAGCTTTGTTTCCTTCTGAACAACTAAGAGGTTCGAAGGGGCTTCTTGGC ATAATGACATTTCTGCTCTCTGCGTTAGCCATGACTGTGCAGGTCTCTGCCCAGAAGGGACTGATCAAGA GTGAGCTGAGCGCCAGGCAGCCTCTTCCAGCTAACCTTCCAAAGGCACTTCTGAGCGTGCATGTTGGTAA CTCCTGGACTCACCAGGGTAGGCGCTCCTGCCGGGACTTCTGTTTTCCTCCATGTCCTCCATGGGCAGTT CTTCTGTTCTTTCAATATCATGAAGCTCTGTCTCCGTGGTAACTGCCACACACAGCTCTCTAGCCCTCTA GCTGTACTCAACTCTGCACACCCCCTTTCCCTGAAAATACTCAGGTGGTCAGTGCACTTCGGTCAGTGTC AGTCACAAGAGACAAGCAGCACCAGTGCTCAGGGAGTGGAGCACGGCCGGAAGCAGAGCAGGCATGGCCT CCTGATCATCTCAGCATCTGAAGGAGCTAAGAGCACCAAGTGGGGGACCACGTTGATGGCTTTCCTGTCA TCACTCAAGACTCCAGAAAAGCTAGCCTGTCTCAGCCGGTCCTGGTCCTTGCCCAGGTGCAGTTTCTATG GTTAATATTTTCTTCTTGTTTTTCTCATGTAGAATCAGGAGCAATACACTGCACATTATAAGTAACCAGA GTTGGGTATTTAAAAGAAAAAAACCTAGGAATGTAGGATGGCAAAGATGATATTTGCTTCTCGTGGCGTT AATTCAGTATTGGGAGAGTCAGGCGGTGTGGGGGGCATTCCCAAATGGGAAATCATTCTTAACTTAGAAG ACTCTTTTTCAGATTCTTGTTACTCCAGGGAGACTAGTTTTACCTTTGAGCCCCAGCAATGGGTAGTTTC AGCTGCCAGGGGCTGCAATCCACCTTGTCCTCAGTGTTTTCCACTCTAGGGATTCAGGACAAGTCTGTCC ACGTCTCCTTGGCGTGGGTGTTTGTCCAGTGAACAGACACTCAGTTCTAATCTCATGTGTCTGCATTTGT TTCTGAGTCTATATTTGCTTTCTCAGAGCATAGCAAGGTCAACTCCTAAAAATGTACCCAGCAGAAGACC TTTTTGAAAAACCACACACTTGCTTCCTCTGTATTCACACACAAAGTGGTGGTATCATACATTATGCCCT GTGATGTAAGTCCCCTTAGTGCTCAAAGCTGCCTTTGTCTCTTAAGAGCGTGCACTGAGAAAGGCTGTGG GAACGGAAGGCTTATTCTCAGACAGGGTGGTTTGTTTACCCCATGTCTGGACTTGTCCTGAGTCTGAGGA CCACTCTGCTGCACTGCCCGTCCTGATTAAAGACGTCTCTGTAGATGCTAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_029929
• Insert Size: 1797 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC046417, AAH46417
• RefSeq Size: 3853 bp
• RefSeq ORF: 1797 bp
• Locus ID: 77573
• UniProt ID: Q9D2N9
• Cytogenetics: 5 63.03 cM
• Gene Summary: Plays a role in vesicle-mediated protein trafficking to lysosomal compartments including the endocytic membrane transport and autophagic pathways. Believed to act as a core component of the putative HOPS and CORVET endosomal tethering complexes which are proposed to be involved in the Rab5-to-Rab7 endosome conversion probably implicating MON1A/B, and via binding SNAREs and SNARE complexes to mediate tethering and docking events during SNARE-mediated membrane fusion. The HOPS complex is proposed to be recruited to Rab7 on the late endosomal membrane and to regulate late endocytic, phagocytic and autophagic traffic towards lysosomes. The CORVET complex is proposed to function as a Rab5 effector to mediate early endosome fusion probably in specific endosome subpopulations. Required for fusion of endosomes and autophagosomes with lysosomes; the function is dependent on its association with VPS16 but not VIPAS39. The function in autophagosome-lysosome fusion implicates STX17 but not UVRAG.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a).