Champ1 (NM_181854) Mouse Untagged Clone

CAT#: MC202100

Champ1 (untagged) - Mouse zinc finger protein 828 (Zfp828), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Champ1
• Synonyms: AA675043; AI116001; D8Ertd457e; D8Ertd569e; mKIAA1802; Zfp828; Znf828
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC028991 sequence for NM_181854
  CCGCCATTCCCGAGGCCGCACAGCCAGCCCAGAGTTTCCTGTGGGCGTTTCCTCCTCTTCACTTCAGGAC GCCTCGCCGGATGCACTGTTGAAGTCTTTCAAGTGCAGTTCTACGGCGTGTGACAGAAGATGGAAGTGTG TCAGGAATTGAGGAAACCAGCGCTAAGCCTGGAGTGTGGGCACTGCAGTTTCAGAGGCACCGATTATGAG AATGTGCAGCTCCACATGGGCTCCATTCATCCTGAGTTCTGTGATGATATGGATGCCGGGGGCCTGGGCA AGCTCATCTTTTACCAGAAGAGTGCAAAGCTCTTCCATTGCCATAAGTGCTTCTTCACCAGCAAGCTGTA CGCCAATGTGTACTATCACATCACGGCCAGACACGCAGCCTCGGACAAGTGGAGTGAGCAGCCGAAAGAG CAGCCGAGCAAAGACACCGAGTCTGGGAAAAGCCCTTCCCCTCCTGAGCGGCAGAACCCAGCCTTTGACC CAGCAGAGGCGAGGCCCACTCCAGCCCTCCCCATGGAAGCACAGAAAACGAGCCCGAGCTTGTGTCCAGA GTCACAGGCTTCTGGTCCTCCAGTTCTGGAGCCTCAGGGCGCTGGTCCTCTTATTTCGCCCGAGCCCCAA GCCCCTTGTCTTCCTGCTGAGGCCTCAAAAGCTGCTCCTGTTCCTTGCCCTGAGCGTGTAGACCCACCCT GTGAGCTTCCTGAGCTGGAGAAGCCTGAGCGAGGCCCTTCTCCTGAGTCAGTCAAGTCTGCTCTTGTCAG CTCCAAACCCCCAAAGCACTCTTCCTTTGCAGACACAGGGGCTGCCCCTTCAGCTTTGTCTCCAGAGTCA CCAGTACTGGCCACTTCCCCTGAACCTTGGGGACCATCCCTCAGTGCATCTCCAGAGTCTCGGAAGCCAG CGAGGACTGCCTCCCCTGAGCCAAGGAAGCCATCCCCAGCAGAGTCTCCTGAACTTTGGAAGCCATTCCC TGCCATCGCTTCAGAGCCTCGGAGACCAACCCCAGCAGTGTCGCCAGGTTCCTGGAAGCCAGGGCCGCCT GGTTCTCCTAGGCCTTGGAAATCCAGTCCTTCAGCAACATCAGGACCGTGGAAGTCATCTAAACCTGTTC AACCTATGTCTCCTGGACCTTGGAAACCAATACCTTCTGTATCACCTGGGCCTTGGAAACCTGCCCCATC TATGTCCACTGCATCCTGGAAGTCTTCAGTCTCATCTGGTTCCTGGAAAACACCACCCACATCTCCAGAG TCATGGAAGTCTGGCCCACCTGAACTCCGGAAGACAGCCCTTCCCTTGTCTCCTGAGCACTGGAAGGCAG TGCCTCCCGTGTCTCCTGAGCTCCGCAGACCAGGCCCACCACTTTCCCCAGAGATCCGAAGTCCAGCAGG TTCTCCAGAGCTCAAGAAGCCTTCAAGCTCACCAGACCTTTGGAAGGTTTCTCCTGACCAGCGGAAAACT TCTCCTGCTTCACTTGATTTCCCTGAGCCCCAGAAAAGTTCTTGTGGTAGTCCTCCTGATCTCTGGAAGT CTTCCTTCATTATGGAGTCTCAGAAACCTAACGTCTTCTCTGAGACGAGGAAGCACACTGCTTCGGGCTC ATCTGAGTCACCGAAGGTAGCGTCAGACATATGGAAGCCTGTCTTGTCTATCGATGCTGAGCCTAGGAAG TCCACACTGTTTCCTGAGCCCACCAAAGCAGTCCTTCCTGCTTCTCCCGAGCCACGGAAACGCGCACTTT TCCCAGAGTCCCGGAAGCACGTGTTTCTACCTGAACTTCCCAAATCCGCTGTGTTCTCAGACGCCCAGAA GGCCCCTGAGCTTAGTGAGGAGATACAACTTGAAGCTGTGGATAATGCAAAATGCGATAGTCTGGCCCAG GAAGGGCTTCTGGCCACCCCCAAGAAACTGTTAGACGAGGCTTTATCTCCTTCTTCAAAGAAGCTCAAGA AGGACAGCCAGGAGAACTCGGATGCCGAGCTCAGTAGCAGTGAATACATCAGAGCAGATTTAGACACGTT GGACACGAAGGGCCAGGAGTCAAGCAGCGATCAGGAACAGGTGGATGTGGAATCGATTGATTTTAGCAAA GAGAACAAAATGGAGATGGGTAGTACAGAGCAAGCCAAAAATGTGCTTCAGTTCACCGAGGAGAAGGAGG CCTTCATCTCTGAGGAGGAGATCGCAAAGTACATGAAGCGTGGGAAAGGGAAATACTACTGCAAAATCTG TTGCTGTCGGGCCATGAAAAAGGGCGCTGTCTTGCACCACTTGGTTAATAAGCACAATGTCCACAGTCCA TATAAGTGCACAATTTGTGGAAAAGCGTTTCTTTTGGAGTCTCTTCTTAAAAACCACGTAGCAGCCCACG GGCAGAGTTTACTTAAATGTCCACGCTGTAATTTTGAATCAAATTTCCCAAGAGGCTTTAAGAAACACTT AACTCACTGTCAGAGCCGGCATAATGAAGAGGTGAATAAGAAGCTGATGGAAGCCCTGGAGTCGCCATTG GAGGAGCAGCAGATTTGATCTTCAGACAGTGGCCCTTGCTCTACAGTGTTGCTTGCCAGACTCTTAGGTT GTTGAGTAGCCTAGATGGACCAGTAGCCAGTATGTGTGTAGTGGTGTATCCTGGTCTGTAAGTAGTGTTA CAAAAAATAAGTACTTGGTTATTTTTTCATATGATCCGTTGTTTATGTGGATGGCTATTTTATAAGTCTT AATACATTTCTGTTATAGATTCCAGATGGATATAACTCATTTTCCTTAAGTTTTATTTTTGTTATAATAC AAGGAGTGGGGGAAAATAGGCAGGCCATTTAATAAACAAGTAAGTTTAAACCTAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_181854
• Insert Size: 2409 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC028991, AAH28991
• RefSeq Size: 2868 bp
• RefSeq ORF: 2409 bp
• Locus ID: 101994
• UniProt ID: Q8K327
• Cytogenetics: 8 6.4 cM
• Gene Summary: Required for proper alignment of chromosomes at metaphase and their accurate segregation during mitosis. Involved in the maintenance of spindle microtubules attachment to the kinetochore during sister chromatid biorientation. May recruit CENPE and CENPF to the kinetochore (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 both encode the same protein.