Vstm2a (NM_145967) Mouse Untagged Clone

CAT#: MC201901

Vstm2a (untagged) - Mouse V-set and transmembrane domain containing 2A (Vstm2a), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Vstm2a
• Synonyms: Vstm2
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC027127 sequence for NM_145967
  GACACTTCACGGCGTGGCAACCCGGGTCTGTGCCTTGAAGCCTCCGGATCGCAGCCAGCTCGGTCCATCC CTCACTAGTCGCAATCCCCTGTGTCCAAGCTACTCTTTGCTATGAGCGGCAGCATGCGTGCAGTATCGCG CCCCAGGCTCTGAGAGCAGCCTGCGGACACGCTTGCCTATCTGTCTTTTTAGGTTTTGGGGCTCTGGGCT ACACGGATGTGCCCCACTCCCTTGGCATGATGGGGATCTTTTTGGCGTCTGTTGGATTTATGTTCTTTTC CGTGTTATATGTACAACAAGGGCTTTCTTCTCAAGCAAAATTTACCGAGTTGCCGAGAAATGTGACTGCT ACCGAAGGGCAAAATGTGGAGATGTCCTGTGCTTTCCAAAGCGGCTCTGCTTCAGTGTACCTGGAGATCC AGTGGTGGTTCCTTCGGGGGCCAGAGGACCTGGAGCAAGGCACGGAGGCTGCAGGCTCGCAGGTGGAGCT CTTACCCGACAGAGACCCGGACAACGATGGGACCAAGATTAGTACAGTGAAAGTCCAAGGCAATGATATC TCCCACAAGCTTCAGATATCCAAAGTGAGAAAAAAGGATGAAGGTTTATACGAGTGCAGGGTGACTGACG CTAACTACGGGGAGCTTCAGGAACACAAGGCCCAGGCCTATCTGAAAGTCAATGCCAACAGCCATGCTCG GAGGATGCAGGCCTTTGAAGCCTCACCTATGTGGCTGCAAGACACGAAGCCTCGAAAGAACGCATCATCG GTGGTTCCCAGCAGCGTCCACAACTCTGCCAACCAACGAATGCACTCCACCTCCAGCCCTCAAGCGGTAG CCAAAATCCCCAAGCAAAGTCCACAATCAGGTGCGAGGATAGCTACAAGCCATGGACTTTCTGTCCTGCT TCTTGTTTGTGGCTTTGTGAAGGGTGCTTTGCTTTAATCTAAAGTGCTGACTTTTTTCCAATATCACTTT CTCTTCTTAAAGCAAAGAGCAAATCGCCTGTAAAATCTACGGAGCGGACAGCAAAGTTGACCCTATACTC CAAGCACCATTCTGCACCCCTGTACTCTAGTTATCTACACAAGGAGCATCAGCTTCCGGAAGCATAAGTG AAGACACTGTCACACGCTTTATTGATAATATTTTCTTTGGGAAGTTGCTGATCTTTTATTTCAAGAGAAT TAATGGGAAGAGATAGGACATTTTCCAATTACAAGACCAATTTTTTTCCTTTTATTTCAACAAATAAAAC CTGCATTTCACTGACTGCTCAGGAGTTGGCCTGAATGACATCAGTATACTAAATATTTCCATGGATTCCA CCAATTTCCTAACGAGGGACACCTAATCTTCAAGAAGCAAACAAAGATGGAAAACCTAAGAACCACAAAC TGTCTCATACAGCACCCCAGCTGAGGAACAAAACAAATAGCTAAATGCTGACCATGGCAAATCAACATCA GACAACTTTATTTTACATATGGAATAATCAAAGAAAGTTTTTTTTTACTTCCTTTTTGCCCCCTGGAATT TATCTTGGAGTTTCCCTTTTTTCCTTGATTGCCGTTTTCGTTCAATGGTAGCAAGTGCCAATTATGGCCA ATCCTTGTCAATCCTGGAAGGTTTATATTCATATACATTGAGTGTGGTATATATCAATGTATTTTAATTC ATTTGGCAATTTCTGTATAGGCAAACCTGGCAAATTCTGTAAATTGCTTATAGTATGTGTGATATGACTT CAAGGTAGATAGGCTATGATGCTCATGCAAGCTGACTTTCTTCATTCTATATACAAATATATTCATGAGC ATATATTAGGCCACCAACTTCTTTTCCTAAAGAATTATTTTTCATTTGTACCTCATGTATTTTGTGAATT TTGTAGTATATTTCTCTGTTCCACTAGTTTGACCGCTACAGTTTGTCTCTGTTGTCCTCTACTTCCTTCT GGAAAAATTTAAAATTGTGTATGTCTCTGATAAATGAATTAATTTTGTTGTGTGTATGCTATGTTGGAAT TTGCTGTGTTCTTTTAAACATGTATTTATTAAGGTTTGGGGATCTTGAGTTGAGTCTGAAGAATGCACAC CTGGTTTTTGACAGAGTTCCTCATGTTACCAATATTCTATCTCAGAGAAAGAAAGACACCAAGTGGGAAA ACTAAGAAGACATTTTGACTTCCCAAGATCCTGGAAGAGCACTTCACACTCTGACTAAATAATGTTGCTT TTTTTGTTCTTCAAGACTTTTTTGTAGCTTTGTCTTTCTGTTAGTTGCTGCTAATTATATTTTAATGTCT ACTAATTAAAAATTAAAATGTGATTGTTGGCTGAATACAATATGCAAATGACTGCAAAGCCCATACTGAA GAAAATAGATGTTTAATCTTCACTCAATAATTATAATTTTAAATAGTTCATCATTATTTTTTGACCTTAT GATATTTTGTTAGACCTGTTCTAATTACATCTTTCTCTGGCAAAGAAAGATAGAACAATCAATACATTCC CTCTTACAGTATGGAATGGTTGTGGCTTAAGAAAGAATGCATCCAGATGGTCTTCCAGAGAGATTATTTT ATTTTCATTATAAAACCAGAAACCATATATGTAGGAATGGTTCATTCCTAATGTAAGGCCATAAATTGTA GCTTGAAGGCAAGGAATACATTTGTTTTTTTATGGTAAAGGACTGGCCTCTGACATGCACTTATAAGCAA TGTGAATATTTTCATAATATGCTTGACATTCTCCTTTAACAAATATTGTTTTATGGTAAATCTTTCCTTG CCATTTTCCTTCTTTCATTTGATTCATTATTTCATTCTAATGAAGAAAATAAAGGTTTAATTATGATACT TTATTAACATACAAATGTATTTTCTTTCTAAGTTAAATATCTGAAAGTTGTATAAAATGATGGTAGAGAA ATATTACTCATTCGGTTTCTTTGAGCTTTAAGAATCCCATACATTGCAGTATATATTAGAATACTGATTT AACATCAAACTGGGGGGGAAAATCATGTATTATACTTTTACTCAATGTCTAGGTAATGAATTCAGCTAAT TTTACAGCAAGCCAAATGTGTACCCGTATCAGTAATGTTCACCATGCTTGTAATAAAAGGGTATATGCTA GTTTTGGAAGAATGCTCATTAGATTCATTGTATCAGTGTCCAAAATAATAAAGACCTGTTTATCAAAAAA AAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_145967
• Insert Size: 708 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC027127, AAH27127
• RefSeq Size: 3229 bp
• RefSeq ORF: 708 bp
• Locus ID: 211739
• UniProt ID: Q8R0A6
• Cytogenetics: 11 A2
• Gene Summary: Plays a role in the regulation of the early stage of white and brown preadipocyte cell differentiation. Promotes adipogenic commitment of preadipocytes by increasing gene expression of the transcription factor PPARG in a BMP4-dependent signaling pathway.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) uses an alternate splice site in the 3'-terminal exon, which results in a frameshift, compared to variant 1. The encoded isoform (2) has a shorter and distinct N-terminus, compared to isoform 1. CCDS Note: The coding region has been updated to extend the N-terminus to one that is more supported by the available transcript and conservation data.