Dkk1 (NM_010051) Mouse Untagged Clone

CAT#: MC201891

Dkk1 (untagged) - Mouse dickkopf homolog 1 (Xenopus laevis) (Dkk1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Dkk1
• Synonyms: mdkk-1
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC050189 sequence for NM_010051
  AGAGACTCCACCAGCGGAGGTCCCGAAGTTGAGGTTCCGCAGTCCAGACTCTGCAGCCAGCCGCCGTGCA AAGTGACCTCTCTTTTCCTGACCTTTGCCTGTTTGCGTCCTTCGGAGATGATGGTTGTGTGTGCAGCGGC AGCTGTCCGGTTCTTGGCCGTGTTTACAATGATGGCTCTCTGCAGCCTCCCTCTGCTAGGAGCCAGTGCC ACCTTGAACTCAGTTCTCATCAATTCCAACGCGATCAAGAACCTGCCCCCACCGCTGGGTGGTGCTGGGG GGCAGCCGGGCTCTGCTGTCAGTGTGGCGCCGGGAGTTCTCTATGAGGGCGGGAACAAGTACCAGACTCT TGACAACTACCAGCCCTACCCTTGCGCTGAAGATGAGGAGTGCGGCTCTGACGAGTACTGCTCCAGCCCC AGCCGCGGGGCAGCCGGCGTCGGAGGTGTACAGATCTGTCTGGCTTGCCGAAAGCGCAGGAAGCGCTGCA TGAGGCACGCTATGTGCTGCCCCGGGAACTACTGCAAAAATGGAATATGCATGCCCTCTGACCACAGCCA TTTTCCTCGAGGGGAAATTGAGGAAAGCATCATTGAAAACCTTGGTAATGACCACAACGCCGCCGCGGGG GATGGATATCCCAGAAGAACCACACTGACTTCAAAAATATATCACACCAAAGGACAAGAAGGCTCCGTCT GCCTCCGATCATCAGACTGTGCCGCAGGGCTGTGTTGTGCAAGACACTTCTGGTCCAAGATCTGTAAACC TGTCCTTAAAGAAGGTCAGGTGTGCACCAAGCACAAACGGAAAGGCTCCCACGGGCTGGAGATATTCCAG CGCTGTTACTGCGGGGAAGGCCTGGCTTGCAGGATACAGAAAGATCACCATCAAGCCAGCAATTCTTCTA GGCTCCACACCTGCCAGAGACACTAAACCGACAGTCTAAATATGATGGACTCTTTTTATCTAATATATGC TACGAAAATCCTTTATGATTTGTCAGCTCAATCCCAAGGATGTAGGAATCTTCAGTGTGTAATTAAGCAT TCCGACAATACCTTCCAAAAGCTCTGGAGTGTAAGGACTTTGTTTCTTGATGGAACTCCCCTGTGATTGC AGTAAATTACTGTGTTGTAAATCCTCAGTGTGGCACTTACCTGTAAATGCAGCAAAACTTTTAATTATTT TTCTAGAGGTGTGCTACATTGCCTTGTTTCTCTTGCATGTAAATTTTTTTTGTACACGGTTGATTGTCTT GACTCATAAATATTCTATATTGAAGTAATTTTGGTTTACAGTTCTTAAATGTGTAACCCTTTCTGCTTCA ATGTCTAGGGGGTCGAATGTAAGGAGAAATGGAATAGTCGGTGATAGGCATCTAAAATTTAACATGAAAG TATCAGTTTATTTCCTGAGATGTGCTCTCTGCTTAGATTCTCTCTCTCTCTCTCCCTCTCTCTCTCTCTC TCTCTCTCTCTCTCTCACACACACACACACACACACACACACACACACACACATCTCTCCTCTCTAGTTC TGTCTCTCTCGGCTGGTAGTCCTTGAAATAAAATTTAACATTTAATAATCATTAAGTTAGAAGTATAGAT ACATTTGGGGCTCTGTGTGTGTGTGAGAGAGAGGGGGGGTTAAAATGATAAGATATATTGTCTGTGTGTG AGTTAGTTTAAAGAGAATGTATCTACATTGATAAAACTCAAATGGCTTTGGTAATATGGGCTTGAATTTG GTATAACTACCTTCAAAACTTCTAACAAGTGTGGTTGGTGCTTTTGCCTATAATCCCAGGATTTTGGAGG CTGAGGCAGAGGGATGCCCATGAACTCAGGTCCATTCTGGCCAACTCTTTCTATAGAACAATGTTCAGTT TCAAAAAAAACATCAACCAAAAGTAGTTAACAACCAATTAATATTCACCTAAGTGACCAACAGTTATTGG AAGGGAGGGAATGCCCAAGCAAGTGATTCCAGATGATGGGCAGAATTCAGAAGAGATTATGCAGAAGGAA GCCTGCCAACTTAAGCCAAATAATTAATTCTGAATATTTTAGATCCTCTGACATGCTGAAGAATTGTTTC AATCACTTTGATTTAAAAAATAATTACTAAACAATCATAAATTCTAGAATGTAACACTTCTCTTGCCAAG AAATTATTTCATTTTTTGCTTCACCTGTACTCCATTGATTGGAATGAGAATGGCTTTATTAAAAAAAAAA AAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_010051
• Insert Size: 819 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC050189, AAH50189
• RefSeq Size: 2245 bp
• RefSeq ORF: 819 bp
• Locus ID: 13380
• UniProt ID: O54908
• Cytogenetics: 19 C1
• Gene Summary: Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6 (PubMed:18524778). Inhibits the pro-apoptotic function of KREMEN1 in a Wnt-independent manner, and has anti-apoptotic activity (PubMed:26206087). Plays a role in limb development; attenuates Wnt signaling in the developing limb to allow normal limb patterning (PubMed:18505822).[UniProtKB/Swiss-Prot Function]