Exo5 (NM_028457) Mouse Untagged Clone

CAT#: MC201633

Exo5 (untagged) - Mouse defects in morphology 1 homolog (S. cerevisiae) (Dem1), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Exo5
• Synonyms: 3110037I16Rik; AV297100; Dem1; Exo V; mExo5
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC023360 sequence for NM_028457
  GTGAAGAGCTAAGGGGCTGCCCCTTTCAGGCACGCCCCTCATTCGGGTTTTTGTTCTGTGCATTTTGGGC CCGCGATTGAGTCTTTGCGCTTGGGCACACCAGGGGGACTGGGGCTGGGGGCTGAGTGGAAAGCACACGC CTGCCTGCGTGGAAGGCCTGTAACGAGTCAGGACGCAGAGGATGAAGGCAGCAGCAGGCAGCCGGAAGCC ATCAGCAAGGCAGGGCCTCCGACCTCAGGCTGCGGCTGGGAACCTGGAGAGCAGAACTTACCGCCTGGAG ACCTTACCCTTGGAGAGGCCAGTGTTAGGAGGATTTATGACACGCAGCTGCCCTAGCAGTGTGACTCAAG TGGAAACACCATAATGTGAAGGAGTGGTCAGGTGAAGAGAACAGAAGTCCACATTCCATCATAGCAAGGT GCCTCCCCATCTCCCCTCGTTTTAATACATGCTCTTCCCTGTGAGGAGGGAAATCAACTAAGGCCCAGGT CCCTATTTGTTCAATCTAGGGCTGTACCATGGCTGAGACTGGGGAAGAGGAGACAGCATCAGCAGAAGCC TCAGGGTTTTCAGACTTGAGTGACTCAGAGTTAGTTGAATTTCTGGATCTGGAAGAAGCCAAAGAATCGG CTGTTTCACTTAGCAAGCCTGGCCCTTCTGCTGAACTCCCTGGGAAGGATGACAAACCAGTAAGCTTGCA GAACTGGAAAGGGGGATTGGATGTCTTGTCACCCATGGAGAGATTCCACCTGAAATATTTATATGTCACT GACCTGTGCACTCAGAACTGGTGTGAGTTGCAGATGGTGTACGGGAAGGAGCTTCCTGGTTCGTTGACAC CTGAGAAAGCAGCTGTTTTGGACACTGGTGCTAGCATCCACCTAGCAAAAGAACTAGAACTTCATGACCT TGTGACAGTCCCCATCGCCACAAAAGAAGATGCTTGGGCAGTTAAGTTTCTGAACATACTAGCAATGATT CCTGCCCTACAGTCGGAAGGGCGCGTCAGAGAGTTTCCAGTGTTTGGGGAGGTGGAGGGAATATTTCTTG TTGGAGTCATTGATGAGTTGCACTACACATCCAAGGGGGAACTAGAGCTGGCTGAACTCAAGACACGAAG GCGCCCCGTGCTCCCCCTGCCAGCTCAGAAAAAGAAAGACTATTTTCAAGTTAGCCTATACAAATATATC TTTGATGCCATGGTACAAGGGAAAGTGACTCCTGCTAGCCTAATCCACCACACTAAATTGTGTCTAGACA AGCCACTGGGACCTTCTGTGCTGAGGCATGCCAGACAAGGAGGCGTGTCTGTAAAATCTTTGGGTGACCT TATGGAACTGGTTTTCTTGTCTCTTACACTGTCTGATCTCCCAGCTATTGATACCCTAAAACTTGAGTAT ATCCATCAAGAGACTGCCACTATACTGGGCACAGAGATTGTAGCCTTTGAAGAGAAGGAAGTGAAAAGCA AGGTGCAGCATTACGTGGCCTACTGGATGGGCCACCGAGATCCTCAAGGCGTTGATGTGGAGGAGGCATG GAAGTGCCGGACCTGTGACTATGTGGACATCTGCGAGTGGAGGAGGGGCAGTGGAGTGCTCAGCTCATCG TGGGAGCCCAAAGCCAAGAAGTTTAAATGAATGAAGGTACACTTTCAGAAATGTTGATCGTTTCTGTTCT TAATATACCCATGTAAGAGGACCAGTCTCTCAGCTTGGCTCTCGTGGACAGTGTTTTTTATGTTTACAAT CTTGAATAACATTCCATCCCAGGGCCAAAGATCAAGAATGTGGAGAGATGTGGTGTTAGAGTGACCTTGG AGCTTTCAGGTAAACTGCAAAGAAGACAGATATACATCAGTGCAGCAAAGGTATCCCTCACTAATCACTG TTGGCTCAAGAAAATTCTTTGGATTCTGATAAATCTTTACTTTTTTCTTGATAATTTTTTTACATTTTTT ACAATAAAAATGAAATGCTGTCTAAAATAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_028457
• Insert Size: 1122 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC023360, AAH23360
• RefSeq Size: 2004 bp
• RefSeq ORF: 1122 bp
• Locus ID: 73172
• UniProt ID: Q9CXP9
• Cytogenetics: 4 D2.2
• Gene Summary: Single-stranded DNA (ssDNA) bidirectional exonuclease involved in DNA repair. Probably involved in DNA repair following ultraviolet (UV) irradiation and interstrand cross-links (ICLs) damage. Has both 5'-3' and 3'-5' exonuclease activities with a strong preference for 5'-ends. Acts as a sliding exonuclease that loads at ssDNA ends and then slides along the ssDNA prior to cutting; however the sliding and the 3'-5' exonuclease activities are abolished upon binding to the replication protein A (RPA) complex that enforces 5'-directionality activity (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same protein.