Lig1 (NM_001083188) Mouse Untagged Clone

CAT#: MC201514

Lig1 (untagged) - Mouse ligase I, DNA, ATP-dependent (Lig1), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Lig1
• Synonyms: AL033288; LigI
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC028287 sequence for NM_001083188
  GAGCGAGCGAGCGAGCGAGTGAGCGAGTGTGGCGGCGTCCGGCAAAGTGATTTGGAGAGGTACAACTGAT GAGAAAAAAAGAGCAAGAGAGGAAAGGAGAGACTTCTGCTGCCAACATGCAGAGAAGTATTATGTCATTT TTTCAACCCACAAAAGAAGGTAAAGCGAAGAAGCCAGAGAAGGAGACACCCAGCAGCATCAGAGAGAAGG AACCCCCTCCAAAGGTGGCGCTGAAGGAGAGGAATCAAGTGGTGCCCGAGAGTGATTCTCCAGTGAAGAG GACAGGAAGGAAGGTAGCCCAGGTTCTGAGCTGTGAAGGGGAGGACGAAGATGAAGCCCCTGGCACCCCC AAAGTCCAGAAGCCTGTGTCAGACTCTGAACAGAGCTCTCCTCCCAGCCCTGACACATGTCCTGAGAACA GTCCTGTCTTCAACTGCAGCTCCCCCATGGACATCTCCCCATCAGGATTCCCAAAGCGTCGAACTGCGCG GAAGCAGCTCCCCAAACGGACAATTCAAGACACTCTTGAGGAGCAGAATGAGGACAAAACCAAAACAGCT AAGAAAAGGAAGAAAGAAGAAGAGACCCCAAAAGAAAGCCTCGCAGAGGCTGAAGACGTAAAACAGAAGG AAGAAAAGGAGGGGGACCAGCTCATAGTCCCCTCTGAGCCCACAAAGTCCCCTGAGTCAGTAACCCTGAC AAAGACAGAGAACATTCCAGTGTGTAAGGCAGGAGTGAAACTGAAGCCTCAGGAAGAGGAACAGAGCAAA CCTCCTGCTAGAGGTGCCAAGACACTGAGCAGTTTCTTCACACCCCGAAAACCAGCAGTGAAAACTGAAG TCAAACAAGAAGAGTCAGGCACTCTAAGGAAGGAAGAGACCAAGGGAACCCTGGATCCAGCAAATTATAA TCCTTCCAAGAATAACTATCATCCCATTGAAGATGCCTGCTGGAAACATGGCCAGAAGGTCCCTTTTCTC GCCGTGGCCCGGACATTTGAGAAGATTGAGGAGGTTTCTGCTAGGCTCAAGATGGTGGAGACATTGAGCA ACCTGCTGCGCTCCGTGGTGGCCCTGTCCCCTCCAGACTTGCTCCCTGTTCTCTATCTCAGCCTCAACCG CCTCGGGCCACCTCAGCAGGGACTAGAGCTGGGTGTTGGTGATGGTGTCCTCCTCAAGGCCGTTGCCCAG GCCACAGGTCGTCAGCTGGAGTCCATCCGGGCTGAGGTAGCTGAGAAGGGGGATGTGGGGCTGGTGGCCG AGAACAGCCGCAGCACTCAGAGACTCATGCTGCCTCCACCTCCACTTACTATTTCTGGAGTCTTCACCAA ATTCTGTGACATTGCCCGGCTCACTGGCAGTGCTTCCATGGCCAAGAAGATGGACATTATCAAGGGCCTT TTTGTTGCCTGCCGCCACTCAGAAGCCAGGTACATTGCCAGGTCCCTAAGCGGGCGGCTGCGCCTTGGCC TAGCTGAGCAGTCGGTACTGGCTGCTCTTGCCCAGGCTGTGAGCCTCACACCCCCTGGCCAAGAATTTCC CACGGCGGTGGTGGATGCTGGGAAGGGCAAGACAGCAGAGGCCAGAAAGATGTGGTTGGAAGAACAAGGC ATGATCTTGAAGCAGACCTTCTGTGAGGTTCCTGACCTGGATCGAATCATCCCTGTGCTGCTGGAACATG GCCTGGAACGTCTCCCAGAGCACTGCAAGCTGAGCCCAGGGGTCCCTCTTAAACCAATGCTGGCTCATCC CACTCGGGGTGTCAGTGAGGTCCTAAAACGCTTTGAGGAGGTCGACTTCACCTGTGAATATAAATACGAT GGGCAGCGAGCACAGATACATGTTCTGGAAGGTGGAGAAGTGAAGATCTTCAGCAGGAACCAGGAAGACA ACACAGGAAAGTATCCTGACATTATCAGCCGCATCCCCAAGATTAAACACCCCTCAGTCACATCTTTCAT TCTGGACACTGAGGCTGTGGCCTGGGACCGGGAAAAGAAGCAGATCCAACCATTCCAAGTACTCACCACG CGCAAGCGCAAGGAGGTTGATGCATCGGAGATACAGGTGCAAGTGTGTCTGTATGCTTTTGACCTCATCT ACCTCAACGGAGAGTCCCTGGTTCGCCAGCCCCTGTCTCGACGCCGGCAGCTGCTCCGTGAGAACTTCGT GGAGACAGAGGGTGAGTTTGTCTTCACCACCTCTTTGGACACCAAGGACACCGAGCAGATTGCTGAGTTC TTGGAGCAGTCAGTGAAGGACTCCTGTGAGGGCCTGATGGTGAAGACCTTGGATGTTGATGCCACTTATG AGATTGCCAAGAGGTCACACAACTGGCTCAAGCTGAAGAAGGACTACCTTGACGGTGTGGGTGACACTCT GGACCTTGTGGTGATTGGTGCCTACCTGGGCCGGGGGAAGCGGGCTGGCCGGTATGGAGGCTTCCTGTTG GCTGCCTACGATGAGGAGAGTGAAGAGCTTCAGGCTATATGCAAGCTGGGAACTGGATTCAGCGATGAGG AGCTGGAGGAACACCACCAGAGCCTGCAGGCCCTGGTATTGCCTACCCCACGCCCCTATGTGAGGATTGA TGGGGCAGTGGCCCCAGACCATTGGCTGGACCCAAGCATTGTGTGGGAGGTGAAGTGTGCTGATCTTTCC CTGTCCCCCATCTACCCTGCTGCACGGGGCCTGGTGGACAAAGAAAAAGGGATCTCTCTTCGTTTTCCTC GGTTCATTCGTGTCCGTAAAGACAAGCAGCCAGAGCAGGCCACTACCAGTAACCAGGTTGCCTCTTTGTA CCGGAAGCAGAGTCAGATTCAGAACCAACAAAGCTCAGACTTGGACTCCGACGTTGAAGACTATTAACCT CCTGCTCTCCTGGGCCTGGGGTGGAGGTTGCGAGAACATGGGCACTGTTGGATTGGTGGTGTTGATTGGT GTGTGTGGAGTCACAGGAGTGAGATCCTGGGGCTGGAATGCTTTATTTTTTTCAATAAATGACTTTTTAG ATGCCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_001083188
• Insert Size: 2751 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC028287, AAH28287
• RefSeq Size: 3050 bp
• RefSeq ORF: 2751 bp
• Locus ID: 16881
• Cytogenetics: 7 7.82 cM
• Gene Summary: DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) uses a different segment for its 5' UTR, compared to variant 3. Variants 1, 2, and 3 encode the same protein.